LOCUS       sequence-172628-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-207
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(129..148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(808..1599,1600..1668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1431..1450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1669..1773)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1799..2254)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1936..1957)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2148..2167
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2381..2403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2395..2412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2396..2412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2419..2438
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2419..2437
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2470..2486
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2470..2486
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8292..8308)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8293..8309)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8337..8355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8376..8392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8376..8392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8389..8411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8400..8416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8424..8430,8431..8448,8449..8454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8469..8490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8607..8624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
       61 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      481 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
      901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
      961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     1801 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1861 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1921 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1981 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     2041 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2101 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2161 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2221 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     2281 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2341 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2401 acgacggcca gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt
     2461 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgatat caagcttcat
     2521 ttatttcgta tgtttaacta ataaaaattt ataaaagaag catgactttg tgtgtcgaat
     2581 gttacagata caaagatttt aattttgaaa gagttgtatt tatcgattcg gagaactcgc
     2641 tgatttgttc tcgagctaga gtgacatctt ttatcgctgc gctggccgtg ttagctttta
     2701 tgtctggact ggtttccagt acgttggtat cagtggatag gatgattgat tggctcttgg
     2761 gcataagtcc agtaacggta ggctgtttaa actttggttc ggagaggttt ttggtggtta
     2821 aagtttcaga gctaaggttg tatgggcgag tagacacagt tttattgggc ggaagttggt
     2881 ttaaaatatc tgttgttttt gggtagagat gaaaggaaat taaatctgtc gcttcgtatt
     2941 cttgcacggg aaagtggaaa ggatttttta tgtagaaagc gacttttttt ggatcagtgg
     3001 acatcggcgg tagataaacg ttgacaactt cgttgtcata attaaatagt atcaggggaa
     3061 tattgggtgt cgctctgttt gtgtggttga ggtaggagac gattttattg agctgtactg
     3121 aaaagattga atgttcgact atgttcttag tccatgactg tttcaggatg tagttgatgt
     3181 cgaatgtgcg tgctctgagg acggattgga agtctttatc gtggagtacg ttttccaggt
     3241 gtctcatgca aagcaggtat gttttttcga tggtgtcgat gtacatagcg aatgtcattt
     3301 cgtaccccgt caagaaagag taaagaatgt agtcgaagac atattcctga gcctgcaggc
     3361 gagtgagtgt cggtgtgttt tgtgctttgt gtacggtgat ttttgaggta atcttttctc
     3421 cggattttat tgtgtccggt tcgtggggag ttaccgagga catggtgatc ttattttggt
     3481 gtacgtattc ggagcatagg tccttaatta agttttgata agatctttgg gtaaaagtga
     3541 tcggtttgta ttgaatccaa atatagttca gaaatttgta ggggagaatt aagaaggcag
     3601 tgagatagat aaaaaacttt tcgttttggg ggcacagttg tgtaaaaaat gtttgttgga
     3661 aggtatcttt cataagtttg gcggattgcc atagctgggt gtcgatacat atacttctca
     3721 tcaaatgcat gggcttctcg ggagggttgt ggctaacgac gaatgtgtca tagagactgt
     3781 ctttgtctat gcttttggct aatgaattta taaccagatc tgttggatga ctgagacagc
     3841 cgtagagata ttcaggatgt gagcatagta tcaaaagtgt gaattgcttg atgggcaatt
     3901 cgaacgctgt ggaaacagta ctgtcttcag atgctgtcca ggcttgtgtt atgatgtata
     3961 taaataattt caagttcacg atcggtataa gttctctttt aaaattttgt gtaccgtctc
     4021 tgaggtacgg gcctaggtcc agagtgatta tattctccca gaactgctgt agtataatta
     4081 tgaaagattt acacacggac aggagtttat attggctaga taccaggcgg ggggtgtctt
     4141 ctggtacatg aatctgcttg tagttcaggg agaaaaactt cactacggcg ttgtaaaaga
     4201 tgttgtggaa ttgcagcgga actcctgttt cgtcaacaat tagttgagtt ccaaagacag
     4261 agtttacttg gtagaacact tgacggtccc gttcaatcct gaggcgggca tataaatccg
     4321 aaataggctt gacattcgct actgagtaat cgatcgggat gaaattttgt tgctgaaata
     4381 ccgcccattg tttcagaagt gtgttctgga tgtagttttg cgcagccgtt atgaagttga
     4441 ggagtgcgcg cagaccgtta atataatttt ctgtgatgag tgagtcagaa aataggtcat
     4501 tttcctctgg aaatttttca aatggctggc ctttgggcag cgacttgaac ttctccatta
     4561 gtcgttccac attttgtcgt tgcttaggaa agttgaatgc taacttgaag ctggcgacat
     4621 tattgacggt ctcgaagtat tcttttttga gttgttcttt ttcgtaggtc tgttgcatag
     4681 aagtttcgga agtgagaatc ttgttcatga gagtttgata ctgtttctgg ccaccggcaa
     4741 cgcgtttagg atctaactcc ttcaagagct tagtcagttt cggaatgttt ttcgtgttct
     4801 cgacggtttc gttgcagatg actttgtttt caatttctac gtacttggtg ttaatctggt
     4861 attccaaatc tcgtagggcc gcgtaattga acgggattgc ttctttcccg aattcgctta
     4921 tagctgacag gcagaatttg ttgatgtcgt gtactaattg tgttaaccat ttgagagtta
     4981 tctctgcttg aatgtaaggc atctcgttgg ctgctttgtt catcagctga cttataactt
     5041 taaagggatc agaaataaag tcctgcagtt tgatgtcaac gaagtctggg aagtagagcc
     5101 tggaatccga aacaaaggct gtccattttg tatcgaatga aagttttgtt atatggttct
     5161 gatccatttt cgcctttagg tgtgcgttaa agtggtttaa cagctcctgg agttgatttt
     5221 ctttgttgtt gacttcgacc gtgagagctt tagcgaatgc cgtttctctg aattgggtgt
     5281 tttttatttc tttcagcgtg tgcgtattta ggactagata aattttattg gtttttgcat
     5341 cttgtgacga gagaagaatt tttttctcag ctactgcgta ttctttggag agctgttctt
     5401 ttattttgtt cgtctctttt ttcagctctg tgatgcgttc ttcgtagagg tttaattctt
     5461 gtccgtagac ggagtggctg attctagtat atttattagt ggaggtagca attttcgtga
     5521 ggaacgtgat acttttttgc atttggtcga tctctgtttt tacatcttct gtgcagaatg
     5581 ttgttttttt gaacttggat aagaaactga gtaaaggggt atatttttgt tcttccttgg
     5641 acatctgttc ggggtttaga ggagttttga ttaatatctt ttcgagaatg tcggagatgg
     5701 tcttgctgat ggtcgtttct atgtccttta acactggcag tagtgcatga gtgaagtgaa
     5761 tgatgagatc gttatcctgc aattgtttta gaaaggtttc taaaagattc aaatgcactg
     5821 tttccaaaga cgagaattga ccgtctttta gaatgagtaa gatgtcgttc agtctttttt
     5881 cgagggtgtt tctgattgtg aggatgtgtt ttttgtcgtt ttcagcagtt tgtttcattt
     5941 gctcttccat atgtcgttgt agtgctttat cgagctcagg cttgcaagtt tgtaaggcgt
     6001 gtttagtggg tgcagtggct aagaaggctg tcagttcttc gggagatgta atgacggctg
     6061 tttttgctct ctgtatccaa tttttctctt cagcggtgat tattttttga gagtagagaa
     6121 atttgatatg atccatcagc gttggtattt tttttccaaa atgctgtttc atcatgtttt
     6181 ggagttgttc gctgttcgtc gtgatgaact gggccagatc gtcgaattct tctagagttt
     6241 ttaagttagc gcatttcttt gctaattgcc gtagtgtgag aacggattcg aacagtgctg
     6301 ttttttcggt gctgtttggg aagagatcta caagaaatag aacggttgaa aacgtctgct
     6361 cagggactgt tatgtcattg agcatgtcgt ctatacatat gtcaagtatg gattgtattt
     6421 tatcgtggag aatcgcagcg atatctggtc gtttttctaa gatgaaatgg aggtgtaaat
     6481 tttttacggt cgtagtatta ggtaggttat aagcagagag atggtgcagg aaatcgtaaa
     6541 taggggttaa ctcttggtcc ccaaggattt gttttgtgat ttcagctgtt tgcttgtttt
     6601 cggtggtgga gatttccatt ttccccagtt cttccagttg tgatttgatc atttctatat
     6661 ttacgattgt cttgtttttg ctggggtccg ctaagatgga ttcgattgcg gctaggattt
     6721 cggaagctct gtccgagggt ttttgactgt tatagaaatc gacgaacatt tttctgatag
     6781 atagggtaaa gttcgaaagg ttgtttatat tgaagtgaga agttccgaag cgtagaaatt
     6841 gcgtgttgtc atacagtttt tccacttctt gaagcaactc agtgttaatg tttaggccgc
     6901 tgtgttttaa aacttcaatg ttaaaataaa ggtttaccag gtaggcaaat aggcaattca
     6961 gttcggtaac tggcatgaaa ccggcagcga gattgttata gttatcttgt acgatttttg
     7021 tgaatttgtt tctgagtgtg tggatggtgt tggtgagttt ttcactgatt tgtttttcta
     7081 gggataattg ttgggtgttg ttcagtgtgg agatgtttgg taattcaatc gattcagtca
     7141 tgaaacgcgg aacgatggct tcagtgatgt cgctcgtgtg aactttgtgc acgttttctg
     7201 attcgatagg aagtgcggga gtttcgccga gtttgtcaat aaaattagag agttgtgcga
     7261 aaatattctt gtctcgttca atatcttctt gaatctcttt tcgtttttcg ttgagcagaa
     7321 cagctatgtt gtgtttttct gccttattca gacaaacgaa ttcatggaag tgtttcacag
     7381 ggttctggct cacataggct tttagataat tctctatgtc atctagtttc tttggcatct
     7441 cttggagcat ttgtgtggtc cacgatttga cccattcata atgtttttcg tgctcattat
     7501 cagctattgc cagaaatttg cttagtaaga ttctctcgag tgttcgaaag gtcgtggttt
     7561 tgcacaggta attattgtaa atcaagagga tgtccagatt gttttcttga caagctgtca
     7621 acgcctcgtt gaatacttcg gaaaagtcct tgaaaggcaa taaataattt atgaaaatcg
     7681 tttgctgctt ttgtttgcta gaatcgtcat ttgtggatcc gacgcctaca aacacgtcga
     7741 cgatttgaca caataagtga aaaactctgt tccagagaaa gggtgttgcg aagggctgat
     7801 taggattcat gggagaatct tgtaagtata tggtccagtt gaattgagat ttcattatag
     7861 gaaaagattt gagatcgtga aataaagatt gaaattgtga tcgtagttca taaaagctcg
     7921 gaatcaggtc gacttccgtt gcgtaatatg tcaaggatgc ggtatctttt ttcttcttgt
     7981 catttagttc tagttctaga ctatgagagt ttttctttct tttctggggc gcgcgttcta
     8041 gatatgtcgt tttagccgca acgaggtctt gttgcgatcc gatatcatct gtttttttta
     8101 tatcatggat ttccgtggaa gaaaatttat ctagcgtaat cgctatgtcg gggtctttat
     8161 aagtttttag gataagaagc tcggcgtcgt gactagagag cgacaaattg gaggtttcta
     8221 tgaaaaaaat gaagctggcg tcgtagtaga agccttcagt gccgtgggcg gtgagcagtt
     8281 cataagctta tcgataccgt cgacctcgag ggggggcccg gtaccagctt ttgttccctt
     8341 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8701 gataacgcag gaaagaacat gtgagcaaa
//
