LOCUS       sequence-172629-        7379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6z-225
FEATURES             Location/Qualifiers
     source          1..7379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(824..840)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(824..840)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(872..891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(873..891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(898..915)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(898..914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(907..929)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1055..1510)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1192..1213)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1404..1423
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1537..1641
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1642..1710,1711..2502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1860..1879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2673..3261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3162..3181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3415..3432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3549..3570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3585..3590,3591..3608,3609..3615)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3623..3639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3628..3650
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3647..3663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3647..3663
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3684..3702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3730..3746
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3731..3747
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccggcgacag taaacttttc agcggaattt caaaaattaa ttccacatgt aatttaagca
       61 ttttaaaacg tataactcac aaggtgaaaa catatttacg aatacagtag ttttcgtgat
      121 attttttaaa aattaataaa ttttaaatcg tgtaagtaat aaagcatact attagattct
      181 tcacgttaac agagcaagct cttcgaggtt ttcagtagaa tttcaatatt taattccaca
      241 tgtaatttaa gcattttaaa atgtagaatt cacaaagtga caaaacattc acaaatacag
      301 tagttttcac ggtatttttg aaattaataa attttaaatc gggtaaatgg taaagcatgc
      361 tgttaaattc acgttaacag agcaagttct tcgaggtttg ctatgtgttt atcagtgaat
      421 gtatttagta tttcaatttt agatcatggt tcggtaaaga tataagtccg tgtaaaattt
      481 tttggtttag ttttcatatc tactagactg tgaggtgttt gaagtcgatg gtttagctca
      541 tgtcaaaacg caatgtcaaa ttgtaataag tcgttcattt cgtgtttaga aataaggaat
      601 tggtatttag aatagctttg tcgcattttt agccgcgacg agaaggttat gacgacgttt
      661 tcgattgcat tgtttgaacc tttttcggag tttgaattta ccgtttaaag aaaaacagtt
      721 tcacgaatag aatctaaaac aggcttccaa attttagtta atatttattt cgaatttcac
      781 ttggggtaca gaaagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga
      841 gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt acaattcact
      901 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      961 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1021 ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta
     1081 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     1141 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     1201 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     1261 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     1321 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     1381 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     1441 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     1501 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1561 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1621 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2161 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2221 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2281 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2341 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2401 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2461 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2521 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2581 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3541 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3601 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     3661 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg
     3721 ggccccccct cgaggtcgac ggtatcgata agcttcccta ccatccacaa aaaaaagcat
     3781 atcatccata gtcggcttgt cctccctatc cctcatcacc tcagctatta ttaataattc
     3841 tggatcaata tcgttagtta aattctcaac aatgctgaga accttgccct tgaccacttc
     3901 cgtatcaaac ataattgtct cacgccgaac ctttttcact ataacttctg agaactttgt
     3961 agcaataatc gttttctgcc tcataaatct aaaatcttgc aatgcagaag aggtagggtt
     4021 taagttccta tccacgccac ttccacctat ccaacctaac tggccaaact gaaaatattc
     4081 cctattagcc aagctcacaa atttctctaa tgtcaaacca aatgtaacca aagatcgact
     4141 tgtaaaaatt gtaccaaaat tgggttcttt ggacagtgac gcaatcacag gattcggaac
     4201 gccatccaaa actaactttt taacataaac taaatattcc tgaatagaag aaaatttagg
     4261 ctgcttgtct aacaccacag gaaacaacac aggattctgc gtaaccagct tagatgtaag
     4321 accatgtaag ctagtcaagt agtcatgaaa acctaaagcc gacaaaaact tgttatgaaa
     4381 gtaacaatct ataaaagttg agagacactc tggctgtatg tcgataatgt ctatctcatc
     4441 ataggtattc gtaatactga atgcaaattt gataaaagtc ttaacttctt cggggttacc
     4501 aatatctaaa gttttcggaa cattgttgag cagaaaacgt tgccataact ctaagcacga
     4561 aattttgaca ttcggaaaca atttatcatg atatttaaat aaaagaaagg agatacagcc
     4621 agacaaggga ttcttcttgt gtttcacgtt ctttttaaaa taagggttgt tgtgtgctgt
     4681 cttactggac ttgttgaacg gtctattttt tattctaaaa tctcgaagac attgcataga
     4741 caaacggcaa agtttcgcct gaatcgtagt tttcacatat ttacccgatt tatatagatc
     4801 gcaatctaag atctgctcca tatcaacatt cgaagcgact ttaaccgttc tcgtattcag
     4861 aaaacccttt ttgagataac tgctgtgaaa cgttccgtac agtgattgat actgctgaac
     4921 gagccattta gaaattgaat taccagtaca aggtctgtcg acaacatgac ctgaagccac
     4981 tatcaatgcg aggttctgca aaaccgttaa aatgacctta taatatgcaa acgtcaaaaa
     5041 aggagaaaat gcagttgcat aaggggtcgt atctacatta aatgattgta gacaattttc
     5101 tatctgttct ctaggcgttt gtgtccttct catttcaaca atgcatctag aaacacattc
     5161 ctcgatacat tgtgtcaatc cgtggattat agaaacaaaa tcttttttac ttcggacatt
     5221 gaaagtatct tcgcctgtta caggatcaat cagagaattt tttttacagt aatcgaagat
     5281 ctgagataca aatttacccc tgtccagtga gagcgtgttt tgttgatctt ttccaatctc
     5341 tttgagctcg ctgacaggct tcctgccaaa agaacctaat atatcaacct cggcataata
     5401 ccgagaaaac atgctcataa caagtggttc ctttttaaca ttttttggta ttgccggcaa
     5461 acgagtacca acccgcacca tggcagttcc aatacacgat tgacaacatt tcccatcaca
     5521 gaattcacat aaattcgccg aacaatttac cagatgattg taaatttcag tgtacgcatt
     5581 acccgcgtta taaacgctca tacgatttaa attccacact acataagaaa gcagctgtgg
     5641 gcaagtcgca caggcataag ccaagtgatt agctgaaaat ttgtcatctt tgcctgatgt
     5701 cgaatcacat ttgatgattt ttctattatc gttatacaca tcctcagtca aagacgacaa
     5761 cgcattacaa aaagacaaag atcgcagcag taattcttga gtcatcaccg aattaaacgc
     5821 ttgtttcacg tttggtataa atgccaattt tgagcaatat aagatagaat ttggcgcaaa
     5881 gatcaaaacg gcaagatgac tagacagatg taactttaaa ttcgtgagct gcgccatgcg
     5941 ttcagcgtgt gtctctgagt ttcttatgat tggccagtca ttgaaattca ttatctgact
     6001 ggggtcatac accgaatcca ggtattcggc tacatgacta aacgataatt ctgtcacaac
     6061 ggaatcaacc aacatcaaaa aatctttctc gaccgctgaa attttctgtc cggagatacc
     6121 cagataagtt ttgtggggtg ctaatttgac agtttgctcc ccatcctgtg taaattgttt
     6181 caggccagca tcgattaatt ccttagtatt agaaaatcgt aacgttgtac cccatgatgt
     6241 aaaaacataa taaaaaagcg tttcacttaa cgcaggcaga aaaaatcctc gttcctgaat
     6301 aaattctgta caggaagata aagaaatcgt ttcttcagtt tcaaataaag ttgcagagta
     6361 tagaggaatt ttcacacacg aatactcccc aacttgtact tgaatttgtt cttcaacaca
     6421 aggaattaac agaccagcga acaacaactc tttgaaaccg tttccacacg caacatggca
     6481 aataacagaa tctgcatctt tgccgacagc agaacaaaga gctttgatat cgattacttt
     6541 gcgtgctgta ggcacctcaa acgactgcaa gtgaaatttc tgtctggttt gctcacataa
     6601 tcgaataaga tcaccatgat cttcggccat accaactaac tgctcagttc catggaaaaa
     6661 aaaacacacg ggcataaaag acgtaatttt agttagaatt gtgcctccaa agttagtgat
     6721 aggtgtttta accgtggtcg agaaatcgtt ttctacagtc aaattcatta acaacggcga
     6781 aataactaca ggagaatttc tctccattaa cgataaaaca gataaaacat ccaaaagttc
     6841 tttctctttt ggaaaaacat agatccaagc agcagtagaa acaggagcag aaaccaccgt
     6901 ttcgttttca tcagccatct ttgtggattc gatcacaaaa acaaagataa tgggtttttg
     6961 tggtgaaatc cttatatatt atgtttgacg taacataaca cgcgtcatca aacataaaag
     7021 taaaccacaa gttgaaatat accgttttct atatgagttt tacggacaaa agaaaaacga
     7081 ttttcttatg caaatatttt ccacgcagat gacatgacac gccccttaat ttaaatttat
     7141 gtaaatcgtc gtccacctca ggtacaatag tatattatat atatagtttt ttaataaact
     7201 tattgaggac gggagaacga aggcgtggcg tttacgtcat agcctaatta tacattctca
     7261 gaacaggatt taaaaaggct gcgagcggcc ggctgttcag agggacgctg gggtacgact
     7321 tgagacgttt gactgaaaat gatccttcgt gtactatttt ctacaaaaaa attaatttg
//
