LOCUS       sequence-172631-        8827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-227
FEATURES             Location/Qualifiers
     source          1..8827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(102..118)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(103..119)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(147..165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(186..202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(186..202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(199..221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(234..240,241..258,259..264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    279..300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(417..434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(588..1176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(668..687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1347..2138,2139..2207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1970..1989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2208..2312)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2339..2794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2426..2445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2636..2657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2920..2942
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2934..2951
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2935..2951
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2958..2977
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2958..2976
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3009..3025
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3009..3025
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ccgttttact ctcaatgggt tggaggattt tgaattatat tgcgatatta cggagcggca
       61 gataaacaac agaaaagtca ttgtccagat agaaagctta tcgataccgt cgacctcgag
      121 ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
      181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2761 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     2821 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2881 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2941 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg
     3001 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttgaac
     3061 tgggtagagc cactcacagc tcagcaaggc ctactgcctt aataaactca aactctgtgg
     3121 gcagggcata gctgagtaaa aggtagcaga aacttctgcg gacttaaaca tcccatctga
     3181 cagctctgaa gagagccgtg gtactcccgg catggtgttt gagctctgag aatggacata
     3241 ctgcctcctc aagtgggtcc ctgaacccca tgtagcctaa ctgggagaca actcctagta
     3301 agggccaaca gacacctcat acaggtggga gcccctcttc aatgaagctt acgtgttttc
     3361 ttggtactca gattttgatt tttcagcgct agagatttca gaacgcgctc gtcgtcgaat
     3421 caggtcactc ctgtacgact taaaacagaa gttttcggag caagaaattt ctgttttacc
     3481 ggtagcgtca cagatgtgta tcttctgtgc attatataaa caaaacaaac ttagtctaga
     3541 atacgtttct ggtgacttaa agacttctgt ttttagtcca attataataa aggattgttt
     3601 atgcgtgcag acgacaattt ctacaactca gatgctgcca ggtacaaaga gctcagcgat
     3661 atttccggta tatgaccttc gtaagctgct cagtgcactt gtcatttcgg aaggtagcgt
     3721 cagattcgat atataatgtc tttgaaggac tatctgagac agtcaatttc taaagatttg
     3781 gaggtgagac atcgagattc tttaaagatt agattagggg agagacatcc gttgagtgtg
     3841 catcagcata tgatcgccgc taggcagatc attaaatcgg acaatgcgga acaccagcat
     3901 gtcatatctt ctttgagtgg ttttttggat aagcagaaga gttttttaaa agtgcaacaa
     3961 agagctttaa agcagctaga gaaattggac gttgatgaaa taattgatac ggcggcggaa
     4021 gtgaaagcag tcagtaataa tataaaagaa actcttatgg caagcactga attagaataa
     4081 ttatggacaa cggtgtggag acacctcaag gtcaaaaaac tcagccgata aatttgccac
     4141 cagataggaa aaggttgaga aaacatgacg gacttggaaa aggtgttaaa cgaaaacttt
     4201 ttgccgaaga tagctctccg ttaaagaaac agatccccgc ctgcagcgat atggaaacac
     4261 tttcttcgcc tgtaaagttt ggatgcaagt cgcgaagtgc ttctgctctc gatgaaagtt
     4321 ttggaaaatg taaacacgaa actgcttgcg attgttctgc gatagaggaa ttgctttgtc
     4381 acgagtcgct tttagactcg ccgatgaaac tgtcgaatgc ccacaccatc ttcagctcag
     4441 acaaatggaa actggagcta gagaaaatta tagcttcaaa gcagatcttt ctagacatga
     4501 gcgagaatgt tgaacttgta gcctacggcg agactttgtg taacctgaga attttcgaaa
     4561 agatcagctc gccgtttttg tttgacgtgc aaagcgagga gcgttcgtat tcagtggttt
     4621 acgtccctca caacaaagaa ctttgtggac agttttgtca acctgaaaag actatggctc
     4681 gagttctcgg agtgggtgcc tacgggaagg tgtttgatct agataaagtg gccataaaga
     4741 cggccaacga agacgagagt gtcatttcgg cttttatagc cggtgtcatc cgtgcaaaat
     4801 cgggagccga cttattatct cacgactgtg ttattaataa ccttctgatt tcaaattccg
     4861 tttgtatgga tcataaagtg tctttgtcac gtacttatga tgttgatctc tataagttcg
     4921 aagattggga tgtcaggaat gtaatgaatt attacagtgt gttttgtaag ttagctgatg
     4981 ctgtaaggtt tctaaatctg aaatgtagaa ttaatcattt cgatatctca cctatgaata
     5041 tctttataaa tcataaaaaa gagataatct ttgatgccgt gttggcggat tacagcttgt
     5101 ccgaaataca tcccgagtat aacggcacgt gtgctattgc taaagagtat gacagaaatc
     5161 ttcaacttgt gccaatcagt cgtaacaaat tctgtgacat gtttaatcct ggatttcgac
     5221 cacttgtcgc caatgcaatg atattggtca atgtatgcga ggcttttgat ggtgaaaata
     5281 atcctcttag acactgtaat ttggatctgt gcgcctttgc tcaggtcgta ttattgtgtg
     5341 tcctgagaat gacagataaa cgcggatgtc gcgaagctca gctatactac gagaaaaggt
     5401 tgtttgcgtt ggctaacgag gcctgtcgat tgaatcctct tagatatcca tttgcttaca
     5461 gggacgcttg ctgtaaagta ttggctgagc atgtagtgtt gctagggtta ttgttttacc
     5521 gagacgtggt tgatatatat gaaaaaatat acgattttct agatgaaaga ggggaatttg
     5581 ggttacgaga cctgtttgag gcaacttttt taaataatag taaacttacc agacgtcagc
     5641 caatcagagg aggtcttgcg tctctacagt cgtccgagta tggagaaaaa cttttacatg
     5701 accttagagc gttgttcttg atcacttctt ctgcagatct ggataaagat acatcatctc
     5761 tctttcagat gtgatatagt aatggatctt aatcagatat ctgaaacact aagtgccgtg
     5821 gcagaagaag agcctttaac catgttttta cttgataaac tgtacgccat acgggaaaag
     5881 atcaagcaag ttccattttc gattgttcgc ttgtgtcatg tttactgcat gctaataaaa
     5941 tataacgctt ctaacaataa ttgcattttg ggccgtaaac ttattgagga aatgcagcag
     6001 tttttgtgcg gcgcgagagt agatggatcg gaagacgttt ctatggatat gagtgaattg
     6061 tgcaagctgt acgattactg tccgttattg tgttctgctc tgtgtcgtgc gccttgtgta
     6121 tttgtgaaca agttatttaa aatcgtagag cgtgaaactc gggggcagtc ggaaaatccg
     6181 ctctggcatg cgttacggag atataccgtg acagcaacta agctgtatga catctacacg
     6241 acaagaaatt ttttagaaca taagggacag cagttttttg gggaagcggt gatttacggc
     6301 gcgaaacatg agcgtgttat tagacacctc gtagcgatct tttacgttaa aagggaagtc
     6361 aaggaaacgt tgggattgct gcttgatcct tcatctggag ttttcggtgc gtctctagac
     6421 gcgtgttttg gaatttcatt caatgaggat ggattcctga tggttaagga aaaagccttg
     6481 atttttgaga ttaaattcag atataaatat ttacgggata aagaagatca ctttgtttct
     6541 gaactattaa aaaaccccac ggagaaatct ttttcggatt ttattttatc tcatccggtg
     6601 cctgccatag agtttcgaga aagagggaag attccgtcat ctagagaata tttaatgacg
     6661 tatgattttc aatatcgtcc tcagagaaag ttgcgcactt gccccactcc agctattttg
     6721 acacctcata tcaaacaact gttgtgtttg aacgagacac agacgtctac ggtaatcgtt
     6781 tttgattgta agagccactt gagtgagcag aagctgtctg tgtttcagaa ggctgtgttt
     6841 accgtgaacg tattcgtaaa tccgaaacac aggtattttt ttcagagtct gttacaacaa
     6901 tatgtaatga ctcagtttta tattaatgat catagtaatc cagaatatat cgagagtacg
     6961 gaagtgcctt ctgttcacat tgtgacggct ctcttcagga gaagaacaga ggaagaaagg
     7021 agtttgcatt tggtgattga tgagacggaa tatatagaag aagaaatacc tttggccttg
     7081 attgtgactc cggtggcacc gaatccggaa tttacttgtc gtgttataac agacatatgc
     7141 aatctgtggg aaaataatat ttgcaagcag accagtttac aagtatgggc acaaagtgct
     7201 gtaaaccagt atcttgcggc atgtgtaaga aaaccgaaaa caccctgatg gattacaagg
     7261 gaaatcctat attactaacg acggaattta ctgttttgac cgataccgaa tcggaagagg
     7321 aagggatggc agatcttgaa aagcccctgc ttgaaagagt acaggccaca ttagctgcta
     7381 aatgtgatac ggaagttgaa aaaaaactgc cgcttaaatc aaaaaaatga tagacgacgc
     7441 agatttacga cacgctcgta ttttattttg tggctatttg acaataaaca tgattgtaat
     7501 aaaaaaaagt gtcattcgtt ttcatccatt aggctcgagc tttcagcgtt aagtagttgc
     7561 ttgtaccgac gcttcttttt aactaggtag cgtacgagtc gacaaattat gaaaccgatc
     7621 gaaattatgg cgacgaccga taaattgata ataagtcctg tgctccactc ggattcaaag
     7681 aaagtttcgt actggagaat agggaaagat aggcctacgg cgccacaaaa cataccgacg
     7741 taatagccga acaagggttt aacgtagcga accaggacgg cttctattac tatactatat
     7801 atacaagcca tgacaagaaa ggtattgatg gaagagaaag cgacggtgat ggaacgtatg
     7861 tagatactat ttccgacgcc caggcaagta gtaatgccga gaaacatcgt agagtaacct
     7921 agcatgagtt cgcttagatt gatgacaacg gtcttgaatt gtacggtccc ctttaggtta
     7981 ggatgaattt tctgaaaggc gaaaaacgac ttgtccgctg actgaaattg cgttatcagc
     8041 gtaacgttaa aggtggtgaa acacacgaag aagatggaat aggcaaatgc acctagggcg
     8101 accagtctga aagacagggc ggtgacgaat aactgaaatg tatccatgat taagatgaat
     8161 tgaaagcagg atgaggtgtc ccccatccag gtaatatccc tcgttgtttg gttaacgtta
     8221 gatgttttgt tagaggagaa cacttttgca cagcagacaa tgtaataaat aataatcagg
     8281 aggaataaga tagccattat cagtacgtag catataatct gcacagggtt tatgtaaagt
     8341 tgatgcgtca tctgatatat ttcattatta gcggagagat tgaccgcttt gaggtcattg
     8401 atttcaaagt aggcgcacgg aaatcctaaa tttggaaagt ttatggctat cagatggacc
     8461 gtgacgttta taaatgacaa ggctgcacag attatagaga ccagccagat ccgtaaatta
     8521 atggtgtcta cacgactgga ggccatacca gctctgtgat ggagaacagg tttcagaacg
     8581 atactctgtt ctcagagtgg ttcggccaga gtttgtccga tgtaagattt ccggacaatg
     8641 tcaccgttta ttctcaagcc gactcggcgg tgagttttga aaacgtcagg cagccgataa
     8701 aactggttcg cgcagcgatg ggttcaggga aaacaacagc tttgattcat tttttgaaac
     8761 aggtacccaa ggaactatct gtccttttga tttcttgccg taaaacgttc gctgcggaaa
     8821 ttttgca
//
