LOCUS       sequence-172633-        7702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-232
FEATURES             Location/Qualifiers
     source          1..7702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..27
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(144..161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(315..903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(395..414)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2065..2520)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2202..2223)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2414..2433
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2647..2669
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2661..2678
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2662..2678
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2688..2707
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2688..2706
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2731..2747
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2732..2748
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7523..7539)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7523..7539)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7576..7596)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7576..7594)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7615..7631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7615..7631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7628..7650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7639..7655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7663..7669,7670..7687,7688..7693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
       61 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     2101 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2161 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2221 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2281 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2341 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2401 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2461 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2521 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2581 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2641 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     2701 tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagctttgtc
     2761 cgcgtatact ctgagagggg aatgaattcg gcgttaaacg agataaaaga cgattttgag
     2821 aactgtgaaa cgaaaaacga cctttttaaa ataattgata aaataagcaa aaattgcaat
     2881 tttatagtgg aacaggtcga gtccttgcct cggagggtgg attcagcggc tatcctattt
     2941 gataatctcg cggtggagat atttaacgat gtaatatatc gacaaaatgg agatggcgtt
     3001 gccgcgaaaa tacgacaggg taacgggcag aatattgaca cataagaata accagatgtg
     3061 cacaaccgaa tgttctcaga tgtataattt acacaatcct atcacgtttg agttgggact
     3121 tggaaacgtg tttgtctgta tgcggtgttt gacggttcac cactgtgata tgcaaactga
     3181 ctgtaccatt gtcaacacgc atgaggggta tgtctgtgcg aaaacgggtt tattttatag
     3241 cggttggatg cctacatatg ccgactgttt cttagaacca atctgtgagc cgaatatcga
     3301 aacggttaat gtcgtggtgg tcttgttatc ttatgtatat agttttctga tggaaaacaa
     3361 ggaacgatat gctgccatta ttgatagcat tattaaagat ggaaaattta taaaaaacgt
     3421 ggaagacgct gtgttttata cttttaatgc ggtttttacg aactcaactt tcaataagat
     3481 tcctttgacg acgataagtc ggctttttgt tcagttgatt ataggaggac acgctaaagg
     3541 aacgatttat gacagcaatg taattcgcgt cagtcgtcgg aaacgagaag acagtttact
     3601 aaaaaagatg agattggagt atggaaacgc acttatacta tgacaccctg taccaatatc
     3661 aaggcggagt gtatccggcc catatttgcc tgccgacaga tgcgtatctt ccgatgagag
     3721 tggattgtat cgagtcttta tattttcggt gtgttttttt caagaatggg atgcattata
     3781 ctgaatggag taaattaaag tttactgtga tttcacgtga aataaagttt aaagatgtgt
     3841 taaagaatgc tgactttgac gaacttttta ccggtttggt ggtaatgact attccaattc
     3901 caatagtaga ttttcatttt gatatcgatt ctgtaatttt gaaattggtt tatccgcagt
     3961 tagtgcaccg agaaatagtg ctgaggctct acgatctcat atgcatcaga ccttcgtcag
     4021 accggccgtc ggaagtatca gctaaaaata ttggtattga tttctatcaa ctaacttcac
     4081 agggaaataa acaaacaccc gatgaggaaa aacgttgtct attttttcag cagggacctt
     4141 tagagccacc ctctaccgtc agaggcttaa aggcagctgg caatgcaaag ccgatgcagt
     4201 ttcccgccca tgtcaacgaa aaaatgaccg aatctttttt aagcgatagt tggttcgaac
     4261 aaaaggtcag atgcaaaaaa atattggatt ttacgcaaac gtatcgagtc gtggtatgtt
     4321 ggtacgagct ttcgttttcc cgcgagatgc agatcgagaa taatttactg tccgcttccc
     4381 agctaaagcg ggttaacgct gcggattttt gggatagaac tgatcggtat ttgcgagata
     4441 ttgggagcag ggtattgaca cacatcgtga aaacgcttca gattcataat aggcaattta
     4501 aacagaaatt taattgcaat ttcccagtta atttcagctt tgaacatcta ttatcattta
     4561 tgcagctcgg gaaagatttt tggattttaa acttaacttt agacagctgc attattaagg
     4621 caattatctg tttcctaggt tttcgaaacg ggagaaaatc ttttttagct caagatgaag
     4681 tttggggaga tttaatagac tgttctaaag gatcggtgat ctacggggaa aagatccaat
     4741 ggattttaga ttcgactaac aatttatatt cgacgcgtcg tgaaaaacag aataagtcgt
     4801 gggaattata tgttgattgc tgtgctttgt atgtatctga aaagttagag ttggattttg
     4861 tgctacccgg cggttttgcg atcaccggta aattcgctct tactgatggc gatatcgact
     4921 ttttcaattg gcgatttggg ttatcttaga aattttttgc agaatgaatg taactggttc
     4981 aggatttgta aaaaaacatt ctatcgcgaa tatcgcagcg ttgcgacatc gtctcctata
     5041 ttttcgttga agaataagcc taagaaatat tgcatgcatt gcgagatggt agtgctcaag
     5101 cgaagtcatg aatttatgtt cagccttgcg gtaaacggca tacattttgg acagtttttg
     5161 accggaataa tgaaatttaa gaagaaacaa gttgcggaag ggctctgtta ctatgtattg
     5221 gaattgggaa gcataagtcc tgtcgatttg agctttatcc caaaatataa ttctgactgc
     5281 gttacaagca tgcattgtgt tacaccggag cttatttatg aaaattgctc tattgtgtgt
     5341 cccgaagagg caagtcgcct cactgtaaaa gggctcgggg acaataaatt gattccctta
     5401 ggtgggtgtg gagtatggtg tctgaaaaat ggtggcgatc tgtatatcta tgcttttgta
     5461 ctcgcttacg atctttacgt agcttgttat gacaaaacca tctttccatc tctggcgaaa
     5521 attgtttttg atatgatagc ttgcgattcc gaagattgcg tcttttgtaa agatcacaac
     5581 aaacatgtat cgcaagccgg acatattgta gggtgcgtct ctaatcaaga aacctgtttt
     5641 tgctacacac cgtgtcaaaa aaaaatgact gatattaaca atccggagtt aatctctttg
     5701 ctctgtgatc aggaaattaa taagatagat attatgtatc ccgagaaaaa agcatcgtta
     5761 tcacttgaca ttaattctta cgttcatggg tacctcggcg acgagccttg tgcgttaaaa
     5821 tgtgttaatt ggatgccaat caggatcagc tcagctctga gcaggctgat tattttgtcg
     5881 tgtcctgtgt gtaagcgtgt ggtaatggac taagtgtgcg ttattttctg tattaatttt
     5941 ttgtttctga aaataaagtt gaattgatag tacttacgtg tgtattgtag cagctggcga
     6001 aaagtgctgt gctctttata ttttgatggt cgattgtaat tacattatcc aggcatgtga
     6061 ttgtcttttc tggaaacatt cggcggcatt taaactcgac ttctttcatc acaaagtgag
     6121 atacatgttt ttgatgtgct acgtaaccga tgctgattcc ttcaatgttt ttcaataaaa
     6181 aacttataat tggaactatg aaccacgttt ttccatgtct tctcgggact aggaatacgt
     6241 tagttttttg tttcagcgta ttcagagtac tttcgtttac gaattcaatg tcgaacacgt
     6301 gggtcagata gttgataaca cgattggcta aagccggaag tttggtgacg gcgatgaaaa
     6361 agattacatg tatgagaatg ttcttctgaa atggttccag ttgtattttg cgcgtgtctc
     6421 caaagtcgtt gaattctcct ttgatccatt ttctgaaatc tttgataaaa ccatctattt
     6481 gcaagaatag cggttcacga tatagaatct taaaggattc gagaaactct gtgtattgtg
     6541 attcaaagga tttgtcggaa gcgggtaaga acttcatttc ttgtagcttg cgcgtaaggc
     6601 tcggtagtat agtaagaggc ttacggtttt taagatgtcg ctgtttttca cagaatgtat
     6661 atagaggttt tacctgttgg ttatatgaat gggcaaaccc gagttctggc gtgagcatta
     6721 taaaacgctt cttatagaag atggcactgt tggggtattt ggtagatatc gtagagcagt
     6781 ttctttcgcc tttccatatg atcgcttcat agttgttttt tatgtgggtt atgtcgcagg
     6841 tacgtagcat ggagcccgac cttacgttgg cggcggtcta tcaggcggcg gcgaatctca
     6901 cagagcagga taaggagatt ttttccgaag cggtaaaaac tgcgttttca gtgtgtagtt
     6961 cggcagcccc gagcgctagg ttgagaatga tcgaaacgcc tacacagaat tttatgtttg
     7021 tgacgagcgt tattccttcg ggtgtgccgt ctggtgaaaa aaaaacaaag ttaaatatcg
     7081 atgccgctct ggataatttg gctttgtcgt ttgcgaacaa aaaatcaaag aagatggcta
     7141 gaacgtattt gctacagaac gtttcgcgga ctcaagatca acaagttgcc atttcgggga
     7201 cgtacatttt atatacaaaa aaacacattg aaacgtcttt gatgctcgat aagacgaagt
     7261 tagttaaaca aattcttgag tatgctgaga cccctaattt gttagggtat accgatgtgc
     7321 gtgatcttga atgtttactt tggttggtgt tctgtggccc taaaagtttt tgccagtcag
     7381 acagttgttt cggatacagt aagacgggat ataatgccgc atttccgaat ctattgcctc
     7441 cgtatctgta cgaatgcggc cagaataatg gactgttttt tggcattgtg caagcttgat
     7501 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag
     7561 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     7621 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7681 cataaagtgt aaagcctggg gt
//
