LOCUS       sequence-172637-        8110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-603
FEATURES             Location/Qualifiers
     source          1..8110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    245..266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(281..286,287..304,305..311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    319..335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     324..346
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     343..359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     343..359
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        380..398
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     426..442
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     427..443
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5630..5646)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5630..5646)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5678..5697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5679..5697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5704..5721)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5704..5720)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5713..5735)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5861..6316)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5998..6019)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6210..6229
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6343..6447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6448..6516,6517..7308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6666..6685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7479..8067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7968..7987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
       61 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      241 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      301 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      361 atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gtaccgggcc
      421 ccccctcgag gtcgacggta tcgatataaa aagactgaaa aatccagatc taaacgttac
      481 agaatacgag tatgctctgg cctctaaaaa gaagaccatc caagcattaa cttccgaaca
      541 aataacgcgt gccatagcga aatgtaataa aaacggagaa ggtctttttt ctccagtaaa
      601 accaggatta caaggattat tagaaatctc cgcttccgat aaatacgttc agatacaaga
      661 taagcgcatc tacagaagac aacacctaca caaagattat caccgtccct ttcctgtatt
      721 cagagtgcag cttttacata aaaatatttt ttgcttcggg aactctgaag attggtacga
      781 aaacatggga tttaaccgca tcttacagta tctccccgac gaatatatct ctgatgaagc
      841 tctaactcgc gccatctggc tacaagacac acattttctc tgcgacgact tcgaaaaaca
      901 attctatact acacgacacg aaatattcaa cgaacgcata ccagtcacaa attacatagg
      961 agacttggat cttccgctgc aagatacagc aaccattacg gaggaaacat ttttctcgat
     1021 gtgtcgcctc ataagactta ccctcatcaa cgcctggaaa aaaatttttc caagcatcga
     1081 cacacacaca catcccatct tcttcttcaa aacccagtgc gacacgacta acgacgcact
     1141 agactatact gaagatccaa ccgaaatcaa acaattctgt atttgtcgga aaaagatcgg
     1201 tcttagaata tcaatcccat tacctaatgg cacagcgatc gccgggggag aaccattaaa
     1261 acaattatcc aagattttaa atcacgttat gtgtttagat caagagctta gtcagatcct
     1321 caattcccta acatttcccg gagaatgttt cgacatcggc atttaccaca caggtcactg
     1381 catcagaata ggctatatgt acaagacaga catggacaaa gggaaaatgc tacatggacg
     1441 gctaactccc atatttatcg tcccagaagg ttaccgaaat tcttgcaaga cgtttattca
     1501 gatgcaaatg gatttaaaca accttctaca tcacggtact aagaaggctc ccatcgaaga
     1561 attaatctat agcataacgg ataaaggctg ccctaaagaa aacctaagtt tcatggattt
     1621 aaaatccaga caactgtgga acaaagttaa catagctaca gacactctaa tcaccaagta
     1681 cctgaacacg catggtttta acaacaatgc aacatcagcg gatgactccc ttttatcgtt
     1741 tattagatta atcggatggc caatcattaa aacgcaatta attactcatt acgagactag
     1801 gatagcgcaa cagttttcac aagtaacttt tctcaaaata gattcaaaga accttcagat
     1861 caaaaaaaca caatttggaa gagtttcgga ttttagctgc ctgaacagac aacatagagg
     1921 aaatcgagat aacgtcctag tttacataca actcaaagcc gacggaaatc ggttaattct
     1981 catcttgtgg agcacatgct ttgctactaa atgccagtcc aacagcaaac aagtacattg
     2041 ttccatcgcc ttagagcaat taaaaaacta acttactacc tataaaccca gaccttcttt
     2101 tagtatttac catttgactc caacgcacat gctaactaaa tcgcgccata tcacagcatt
     2161 tccggagatt aaagcattag caacaccgtc cttcgttctt tcacacaact taaccgcaaa
     2221 aaagcgccaa cggaaagcat ctcaaaaaac atataaagag gccaatttat gtcatttcat
     2281 ttattataag caaacatgta tcctcgtgga gtaaaacgct cacaacatga tcgtcataaa
     2341 caaaccgcct tcagaacgat aaaacgttcc atcacgcata gaccaaccag caagttcatc
     2401 agccatttcg ccaaaaattt tcgaggaaaa ctcgcacctc tgaaacaact ggatgaatct
     2461 agactagacg cactaagtct gacagagtta gaacagctga agaccatcat cgaagagaaa
     2521 caacaagaaa aacgcgctca aaacaatgca atcacctttc tgcctaacct tccaacagtc
     2581 ccctttgccg acacaaactt ttcgataaaa tctcttggcc tgagacctta caacggagac
     2641 gcccgtgatc caaaacaacg catccgagac agatttccac agacgcatga acgaatttgt
     2701 ctcctaacta atgacatact ggaaaccgac ctgctgcttc gctatcgaca atgtctcgac
     2761 tcgctcacgc gagaagaaaa tcagcaacta atgggcgacc ggatattctc cctgaccaac
     2821 tcaccttgtc ttgcttttac agttgctaca gtagaagaag cctgcagtta ttttaaattt
     2881 cacgatctgc acaacctacc ggtcaaccca caagacttat tcatgtatac catcaccgtc
     2941 atgaaattcg aatttttcaa caaactcaac atggcgaaat taacctgtgt tttcaacgac
     3001 aacggacacg gcgacatcga ataccgaaaa ctcaggcaac tctgcggtaa acctgtacta
     3061 gatcgagaga tgccaaactc cgaatttgaa gtacaacaac aaactccaga ctcatttaga
     3121 cacccaattc aacaagccat gtccatcgtt gtgacgttcg ctcgaatact acgacaaatt
     3181 aaagaacata tcatacgaac aaagaaaccg caattcatcc gcgatttcga cacagaacgt
     3241 gtagccgagc gatacgaatg cggtctgata agcaggctca tcggaaagca attcagcaac
     3301 cataaatgcg atgatgttag ctgtcaaaat agaatcgaac gaatcatggc tccttggaaa
     3361 ccaagtctat ttttctgcac ttatttcgcc aaagacgcac caaagtttaa gctgtttcca
     3421 aatcttcctc acgaataccg aaatctttct tttacttgtc caaaggtcga catggaacct
     3481 tcatgcagct attcaacaag tcgcgaccta cctcagacct ctcatcgttc acataaaaac
     3541 caaggtacgc ctaaggttaa atccaaagtt tgcgtcgaaa aaccagacac ctcaaaccta
     3601 acaacgacaa aaacaactac cgaaattctc atagaagaat caatggaaac cgacaacaaa
     3661 ataccagatc ctagagaact aaacttcaat caagcaaaac aagacgaaat tgtgattatt
     3721 aacattaatg aaaacgtcaa ctctaaacat gaaagtgaat caagtgttga aatggactta
     3781 gatttagact atgaagctga cacctgtgaa actaacttaa acacatattc ttctgactca
     3841 gaataactat cttactgtaa acaaattctt acaagtatgt tttgtacaaa ggtttttgct
     3901 gtcacactct gaactatttc tttttaaaat caaaaaaatc tcgatgccaa agctttctgt
     3961 accccaagtg aaattcgaaa taaatattaa ctaaaatttg gaagcctgtt ttagattcta
     4021 ttcgtgaaac tgtttttctt taaacggtaa attcaaactc cgaaaaaggt tcaaacaatg
     4081 caatcgaaaa cgtcgtcata accttctcgt cgcggctaaa aatgcgacaa agctattcta
     4141 aataccaatt ccttatttct aaacacgaaa tgaacgactt attacaattt gacattgcgt
     4201 tttgacatga gctaaaccat cgacttcaaa cacctcacag tctagtagat atgaaaacta
     4261 aaccaaaaaa ttttacacgg acttatatct ttaccgaacc atgatctaaa attgaaatac
     4321 taaatacatt cactgataaa cacatagcaa acctcgaaga acttgctctg ttaacgtgaa
     4381 tttaacagca tgctttacca tttacccgat ttaaaattta ttaatttcaa aaataccgtg
     4441 aaaactactg tatttgtgaa tgttttgtca ctttgtgaat tctacatttt aaaatgctta
     4501 aattacatgt ggaattaaat attgaaattc tactgaaaac ctcgaagagc ttgctctgtt
     4561 aacgtgaaga atctaatagt atgctttatt acttacacga tttaaaattt attaattttt
     4621 aaaaaatatc acgaaaacta ctgtattcgt aaatatgttt tcaccttgtg agttatacgt
     4681 tttaaaatgc ttaaattaca tgtggaatta atttttgaaa ttccgctgaa aagtttactg
     4741 tcgccggcaa attaattttt ttgtagaaaa tagtacacga aggatcattt tcagtcaaac
     4801 gtctcaagtc gtaccccagc gtccctctga acagccggcc gctcgcagcc tttttaaatc
     4861 ctgttctgag aatgtataat taggctatga cgtaaacgcc acgccttcgt tctcccgtcc
     4921 tcaataagtt tattaaaaaa ctatatatat aatatactat tgtacctgag gtggacgacg
     4981 atttacataa atttaaatta aggggcgtgt catgtcatct gcgtggaaaa tatttgcata
     5041 agaaaatcgt ttttcttttg tccgtaaaac tcatatagaa aacggtatat ttcaacttgt
     5101 ggtttacttt tatgtttgat gacgcgtgtt atgttacgtc aaacataata tataaggatt
     5161 tcaccacaaa aacccattat ctttgttttt gtgatcgaat ccacaaagat ggctgatgaa
     5221 aacgaaacgg tggtttctgc tcctgtttct actgctgctt ggatctatgt ttttccaaaa
     5281 gagaaagaac ttttggatgt tttatctgtt ttatcgttaa tggagagaaa ttctcctgta
     5341 gttatttcgc cgttgttaat gaatttgact gtagaaaacg atttctcgac cacggttaaa
     5401 acacctatca ctaactttgg aggcacaatt ctaactaaaa ttacgtcttt tatgcccgtg
     5461 tgtttttttt tccatggaac tgagcagtta gttggtatgg ccgaagatca tggtgatctt
     5521 attcgattat gtgagcaaac cagacagaaa tttcacttgc agtcgtttga ggtgcctaca
     5581 gcacgcaaag taatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt
     5641 tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa
     5701 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5761 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5821 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
     5881 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5941 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     6001 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     6061 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     6121 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     6181 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     6241 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6301 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6361 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6421 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6481 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6541 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6601 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6661 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6721 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6781 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6841 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6901 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6961 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7021 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7081 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7141 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7201 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7261 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7321 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7381 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7441 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7501 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7561 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7621 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7681 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7741 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7801 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7861 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7921 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7981 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8041 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8101 gccttttgct
//
