LOCUS       sequence-172638-        6106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-605
FEATURES             Location/Qualifiers
     source          1..6106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2711..2727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2712..2728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2756..2774)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2795..2811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2795..2811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2808..2830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2819..2835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2843..2849,2850..2867,2868..2873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2888..2909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3026..3043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3197..3785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3277..3296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3956..4747,4748..4816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4579..4598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4817..4921)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4948..5403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5035..5054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5245..5266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5529..5551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5543..5560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5544..5560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5567..5586
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5567..5585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5618..5634
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5618..5634
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aagcgaatag tcctcagacg taggcttata agacgcgtgc aacagcgagg gttagacatt
       61 cgcgaagcgc gccgcgttcc taagctggct tgtgtgacct ttatctgata cagacgaatc
      121 cggaaaaccg gtacgcttct ttcactttgt ttagctttaa aaaaaaaaac gatattattg
      181 ttgccgttgt cgtgtgatca tccatatcgt tctttttggg tgtacattgt gacaagtttc
      241 ttttgtgact atattatctt tcattttttt tttgcgagtt gcaacttttg cttactaagt
      301 tttgagtgct atttctctgt cgctgtgagc ccgtaatgga aacgacaaac agccggcgtg
      361 tttcttcggc gcaacggcag attttggcta cggctacggt gcgatttatc gaaggccttc
      421 atgagccggc tcaagattct atgatgtgtg gggatgacga gctgcagtct caggagagga
      481 tgaagaaacg tcaacgtctc tataacaagt ccaagagaga tctcgtcgat ctcttgcgcg
      541 tctatacgtg cctgaatagc gtccgaatgt ttacgttcgt caatttcggc aggcgaattc
      601 ccttggcttg gcctgaagga tacgagctgg ttattaacaa ctgtagagac gagaatgaat
      661 atgccgatga aaagttgggc gaactggccg atctagtgtg ttgccgggag cgcctcgtcg
      721 tactggggta cgtgaagaaa aggggggaga tcggatcgga gccggcttac tggtttctga
      781 aaggtgacat agtcgttctg ctcggcggtg ccggtcgcgt ttatgcgcat acttggttat
      841 tgccgcagca actgtgccgc gtcggtgaca ccattgacga ttttcttcgt aaaggtctga
      901 agaggttcta ttacgcacat cagctggtat catcgctgca gtttgtggtg gaggacaccg
      961 aggtggacgg cgtctgcagt tgtcgggacg tgctgtgctt tcgagaccgg cacataggtc
     1021 gttctttcgc cctgaggtgg cccaagaacg agactctgct ctttcatcgg cggcgggatt
     1081 cttttgtatt cgtcaggtgc gacgaggcac ggcgcaggct cgcggaaatg tgtttcttcg
     1141 gcagcttcgg ctacaagtac tttgcggacg ccggtcggat ttctctctac gtggccgacg
     1201 acggacggat tttcggattc aacgacaacg acgaagacgg acggccgcgc tttgtggcgg
     1261 aaaattttca gcagtttaga cggataggag tcattcagta ttataagagc tacgttttcc
     1321 gtagggagca gcccgaatgg gcgcttcttc cgacttgtca tctctcgcga atgttttatc
     1381 agaaaacttg gaggcaggcc gacgaagatc tgctgtttgt aaaggctaga aacgatgagc
     1441 gtcaggaatt tccggacgcc ggcactagga gtgccgtgca cgacgtagtt cgatagttcg
     1501 ccctaacgtg ggaggttagg tataaaaaaa aaaagaatgg aagcgtggta gatcgagttc
     1561 ttacgggcgc ttttgacgtc tgggatcatt gtatatttta cccggggatg ggatgtgttc
     1621 acagttttgt gggcggtagg ttcacttcca ttttcgtgga agacgtcggg aatatgtcga
     1681 ttcaacgttg tttgtttcta acagttgtag catccttagc gcgatcgtag acgtttatgt
     1741 gagactgccg gtaacgctgc cgttatcgtt ttttttattt tttttttcct tttaatttta
     1801 tctcggattc cgcacagaaa aaaaaatgtt ttgtcgctct ccgtttctcg gtatatcttc
     1861 ttggagtctg gcctctgcgg ctctctgccc ttcttcgtgc tctttccctg cgggacgtga
     1921 tctgcggtgc gatgcggcag tgccggaggt taaatggacg gcgttcgttc ggactctcgt
     1981 cgcccggccg ctatcggcgg acgacgtgag ggacttcgtc tctacttttg cccattgcag
     2041 gttggcgctc tcgtggcccg tgggggcaga actgagattc gccacctccg acatgctcgg
     2101 gatcacgcag gccgagttgg ccaagctgtc tcgcggatac gggtgctgtc ccggtatgga
     2161 tctcacggtc atcggggtca ctatcttcgc agaagtttcc gcgctggtgc tggtcggaga
     2221 gtgcggggag atatacgcgt ttaacggcgt gttcgacgac gcgctgtacc gtctcgcgga
     2281 ggacgcgttc ggtctctgga agcacggctt gcgtcgcttc gagcccgtct acggttccaa
     2341 gtgtctcatg gaaaccggcg cctctttctt cggcggcatg agtggagttg acgacgccct
     2401 ggctttcgcc gtgtctttcg ataaggctct cgtgccgttg ccttggccgc gcggagcttt
     2461 ctttgagttt gccgtaccca ggagggcgga gaagaggtgg cggctcatac ccggcggagg
     2521 cgtcgcggtg gtgatcggca gattcttcgg acgcggcgtt actttacctc tgctcagacg
     2581 acagcgggtg ctcatggatc aggtcggcag ggtgtatgcg gccagtctcg acgggggagc
     2641 cgtggtgcgc ctgtccgata gcttcagggc gtttctcgcc atgggtgtca gaaaactgtt
     2701 caagaaccat cgataccgtc gacctcgagg gggggcccgg taccagcttt tgttcccttt
     2761 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2821 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4921 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     4981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5161 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5281 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5401 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5461 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     5521 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     5581 atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg
     5641 gctgcaggaa ttcgatatca agcttatcga taccgtttct ggcctccacg gtgtcaggat
     5701 caaactgtta gttgtcagtt ctcagttcga gattaaagat cccatgcgcg tccctcggtt
     5761 gtgttttttc tttcttgaga tcgtgacgat tctcgtatcg ggttatgaca aggaacggca
     5821 tcgcgttcgt gcgctcttgg agcatctgtg cctaaaaaag atctgtctag gcgacctgtg
     5881 tgcatccatg cgactcgctc gcgagataag gcacgatgtt ctggagggct tcctcaatac
     5941 tctggagtta tcttacatgt cgaatccggt gaggtttttt cgttttcacg actgcaatct
     6001 ttacgggacc catatgtgga gtggggtgta tccgaacgtc gttccttacg ctgtgcgcgg
     6061 cggcggctcg tttgacatca ggttgcagga gcaccggaga cgtcaa
//
