LOCUS       sequence-172641-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-607
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..85)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..86)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(114..132)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(153..169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(166..188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(201..207,208..225,226..231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    246..267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(384..401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(555..1143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(635..654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1314..2105,2106..2174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1937..1956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2175..2279)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2306..2761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2393..2412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2603..2624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2887..2909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2901..2918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2902..2918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2925..2944
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2925..2943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2976..2992
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2976..2992
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cagttggcca aaggagggcc ggacatggtt tcaatatttc aatccattgg tcgtgtgagg
       61 cgagttatcg ataccgtcga cctcgagggg gggcccggta ccagcttttg ttccctttag
      121 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      181 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      241 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      301 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      361 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      421 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      481 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      601 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      781 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      841 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      901 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      961 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1021 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1081 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1141 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1201 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1261 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1321 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1381 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1441 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1501 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1561 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1621 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1681 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1741 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1801 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1861 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1921 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1981 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2041 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2101 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2161 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2221 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2281 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2761 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2821 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2881 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     2941 agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc
     3001 tgcaggaatt cgatatcaag cttatcgatg ccgctctgga taatttggct ttgtcgtttg
     3061 cgaacaaaaa atcaaagaag atggctagaa cgtatttgct acagaacgtt tcgcggactc
     3121 aagatcaaca agttgccatt tcggggacgt acattttata tacaaaaaaa cacattgaaa
     3181 cgtctttgat gctcgataag acgaagttag ttaaacaaat tcttgagtat gctgagaccc
     3241 ctaatttgtt agggtatacc gatgtgcgtg atcttgaatg tttactttgg ttggtgttct
     3301 gtggccctaa aagtttttgc cagtcagaca gttgtttcgg atacagtaag acgggatata
     3361 atgccgcatt tccgaatcta ttgcctccgt atctgtacga atgcggccag aataatggac
     3421 tgttttttgg cattgtgcaa gcttacgtgt tttcttggta ctcagatttt gatttttcag
     3481 cgctagagat ttcagaacgc gctcgtcgtc gaatcaggtc actcctgtac gacttaaaac
     3541 agaagttttc ggagcaagaa atttctgttt taccggtagc gtcacagatg tgtatcttct
     3601 gtgcattata taaacaaaac aaacttagtc tagaatacgt ttctggtgac ttaaagactt
     3661 ctgtttttag tccaattata ataaaggatt gtttatgcgt gcagacgaca atttctacaa
     3721 ctcagatgct gccaggtaca aagagctcag cgatatttcc ggtatatgac cttcgtaagc
     3781 tgctcagtgc acttgtcatt tcggaaggta gcgtcagatt cgatatataa tgtctttgaa
     3841 ggactatctg agacagtcaa tttctaaaga tttggaggtg agacatcgag attctttaaa
     3901 gattagatta ggggagagac atccgttgag tgtgcatcag catatgatcg ccgctaggca
     3961 gatcattaaa tcggacaatg cggaacacca gcatgtcata tcttctttga gtggtttttt
     4021 ggataagcag aagagttttt taaaagtgca acaaagagct ttaaagcagc tagagaaatt
     4081 ggacgttgat gaaataattg atacggcggc ggaagtgaaa gcagtcagta ataatataaa
     4141 agaaactctt atggcaagca ctgaattaga ataattatgg acaacggtgt ggagacacct
     4201 caaggtcaaa aaactcagcc gataaatttg ccaccagata ggaaaaggtt gagaaaacat
     4261 gacggacttg gaaaaggtgt taaacgaaaa ctttttgccg aagatagctc tccgttaaag
     4321 aaacagatcc ccgcctgcag cgatatggaa acactttctt cgcctgtaaa gtttggatgc
     4381 aagtcgcgaa gtgcttctgc tctcgatgaa agttttggaa aatgtaaaca cgaaactgct
     4441 tgcgattgtt ctgcgataga ggaattgctt tgtcacgagt cgcttttaga ctcgccgatg
     4501 aaactgtcga atgcccacac catcttcagc tcagacaaat ggaaactgga gctagagaaa
     4561 attatagctt caaagcagat ctttctagac atgagcgaga atgttgaact tgtagcctac
     4621 ggcgagactt tgtgtaacct gagaattttc gaaaagatca gctcgccgtt tttgtttgac
     4681 gtgcaaagcg aggagcgttc gtattcagtg gtttacgtcc ctcacaacaa agaactttgt
     4741 ggacagtttt gtcaacctga aaagactatg gctcgagttc tcggagtggg tgcctacggg
     4801 aaggtgtttg atctagataa agtggccata aagacggcca acgaagacga gagtgtcatt
     4861 tcggctttta tagccggtgt catccgtgca aaatcgggag ccgacttatt atctcacgac
     4921 tgtgttatta ataaccttct gatttcaaat tccgtttgta tggatcataa agtgtctttg
     4981 tcacgtactt atgatgttga tctctataag ttcgaagatt gggatgtcag gaatgtaatg
     5041 aattattaca gtgtgttttg taagttagct gatgctgtaa ggtttctaaa tctgaaatgt
     5101 agaattaatc atttcgatat ctcacctatg aatatcttta taaatcataa aaaagagata
     5161 atctttgatg ccgtgttggc ggattacagc ttgtccgaaa tacatcccga gtataacggc
     5221 acgtgtgcta ttgctaaaga gtatgacaga aatcttcaac ttgtgccaat cagtcgtaac
     5281 aaattctgtg acatgtttaa tcctggattt cgaccacttg tcgccaatgc aatgatattg
     5341 gtcaatgtat gcgaggcttt tgatggtgaa aataatcctc ttagacactg taatttggat
     5401 ctgtgcgcct ttgctcaggt cgtattattg tgtgtcctga gaatgacaga taaacgcgga
     5461 tgtcgcgaag ctcagctata ctacgagaaa aggttgtttg cgttggctaa cgaggcctgt
     5521 cgattgaatc ctcttagata tccatttgct tacagggacg cttgctgtaa agtattggct
     5581 gagcatgtag tgttgctagg gttattgttt taccgagacg tggttgatat atatgaaaaa
     5641 atatacgatt ttctagatga aagaggggaa tttgggttac gagacctgtt tgaggcaact
     5701 tttttaaata atagtaaact taccagacgt cagccaatca gaggaggtct tgcgtctcta
     5761 cagtcgtccg agtatggaga aaaactttta catgacctta gagcgttgtt cttgatcact
     5821 tcttctgcag atctggataa agatacatca tctctctttc agatgtgata tagtaatgga
     5881 tcttaatcag atatctgaaa cactaagtgc cgtggcagaa gaagagcctt taaccatgtt
     5941 tttacttgat aaactgtacg ccatacggga aaagatcaag caagttccat tttcgattgt
     6001 tcgcttgtgt catgtttact gcatgctaat aaaatataac gcttctaaca ataattgcat
     6061 tttgggccgt aaacttattg aggaaatgca gcagtttttg tgcggcgcga gagtagatgg
     6121 atcggaagac gtttctatgg atatgagtga attgtgcaag ctgtacgatt actgtccgtt
     6181 attgtgttct gctctgtgtc gtgcgccttg tgtatttgtg aacaagttat ttaaaatcgt
     6241 agagcgtgaa actcgggggc agtcggaaaa tccgctctgg catgcgttac ggagatatac
     6301 cgtgacagca actaagctgt atgacatcta cacgacaaga aattttttag aacataaggg
     6361 acagcagttt tttggggaag cggtgattta cggcgcgaaa catgagcgtg ttattagaca
     6421 cctcgtagcg atcttttacg ttaaaaggga agtcaaggaa acgttgggat tgctgcttga
     6481 tccttcatct ggagttttcg gtgcgtctct agacgcgtgt tttggaattt cattcaatga
     6541 ggatggattc ctgatggtta aggaaaaagc cttgattttt gagattaaat tcagatataa
     6601 atatttacgg gataaagaag atcactttgt ttctgaacta ttaaaaaacc ccacggagaa
     6661 atctttttcg gattttattt tatctcatcc ggtgcctgcc atagagtttc gagaaagagg
     6721 gaagattccg tcatctagag aatatttaat gacgtatgat tttcaatatc gtcctcagag
     6781 aaagttgcgc acttgcccca ctccagctat tttgacacct catatcaaac aactgttgtg
     6841 tttgaacgag acacagacgt ctacggtaat cgtttttgat tgtaagagcc acttgagtga
     6901 gcagaagctg tctgtgtttc agaaggctgt gtttaccgtg aacgtattcg taaatccgaa
     6961 acacaggtat ttttttcaga gtctgttaca acaatatgta atgactcagt tttatattaa
     7021 tgatcatagt aatccagaat atatcgagag tacggaagtg ccttctgttc acattgtgac
     7081 ggctctcttc aggagaagaa cagaggaaga aaggagtttg catttggtga ttgatgagac
     7141 ggaatatata gaagaagaaa tacctttggc cttgattgtg actccggtgg caccgaatcc
     7201 ggaatttact tgtcgtgtta taacagacat atgcaatctg tgggaaaata atatttgcaa
     7261 gcagaccagt ttacaagtat gggcacaaag tgctgtaaac cagtatcttg cggcatgtgt
     7321 aagaaaaccg aaaacaccct gatggattac aagggaaatc ctatattact aacgacggaa
     7381 tttactgttt tgaccgatac cgaatcggaa gaggaaggga tggcagatct tgaaaagccc
     7441 ctgcttgaaa gagtacaggc cacattagct gctaaatgtg atacggaagt tgaaaaaaaa
     7501 ctgccgctta aatcaaaaaa atgatagacg acgcagattt acgacacgct cgtattttat
     7561 tttgtggcta tttgacaata aacatgattg taataaaaaa aagtgtcatt cgttttcatc
     7621 cattaggctc gagctttcag cgttaagtag ttgcttgtac cgacgcttct ttttaactag
     7681 gtagcgtacg agtcgacaaa ttatgaaacc gatcgaaatt atggcgacga ccgataaatt
     7741 gataataagt cctgtgctcc actcggattc aaagaaagtt tcgtactgga gaatagggaa
     7801 agataggcct acggcgccac aaaacatacc gacgtaatag ccgaacaagg gtttaacgta
     7861 gcgaaccagg acggcttcta ttactatact atatatacaa gccatgacaa gaaaggtatt
     7921 gatggaagag aaagcgacgg tgatggaacg tatgtagata ctatttccga cgcccaggca
     7981 agtagtaatg ccgagaaaca tcgtagagta acctagcatg agttcgctta gattgatgac
     8041 aacggtcttg aattgtacgg tcccctttag gttaggatga attttctgaa aggcgaaaaa
     8101 cgacttgtcc gctgactgaa attgcgttat cagcgtaacg ttaaaggtgg tgaaacacac
     8161 gaagaagatg gaataggcaa atgcacctag ggcgaccagt ctgaaagaca gggcggtgac
     8221 gaataactga aatgtatcca tgattaagat gaattgaaag caggatgagg tgtcccccat
     8281 ccaggtaata tccctcgttg tttggttaac gttagatgtt ttgttagagg agaacacttt
     8341 tgcacagcag acaatgtaat aaataataat caggaggaat aagatagcca ttatcagtac
     8401 gtagcatata atctgcacag ggtttatgta aagttgatgc gtcatctgat atatttcatt
     8461 attagcggag agattgaccg ctttgaggtc attgatttca aagtaggcgc acggaaatcc
     8521 taaatttgga aagtttatgg ctatcagatg gaccgtgacg tttataaatg acaaggctgc
     8581 acagattata gagaccagcc agatccgtaa attaatggtg tctacacgac tggaggccat
     8641 accagctctg tgatggagaa caggtttcag aacgatactc tgttctcaga gtggttcggc
     8701 cagagtttgt ccgatgtaag atttccggac aatgtcaccg tttattctca agccgactcg
     8761 gcggtgagtt ttgaaaacgt caggcagccg ataaaactgg ttcgcgcagc gatgggttca
     8821 gggaaaacaa cagctttgat tcattttttg aaacaggtac ccaaggaact atctgtcctt
     8881 ttgatttctt gccgtaaaac gttcgctgcg gaaattttgc accgttttac tctcaatggg
     8941 ttggaggatt ttgaattata ttgcgatatt acggagcggc agataaacaa cagaaaagtc
     9001 attgtccaga tagaaagctt gcatcgtctt acagaaaatt acgatgtttt aatattagat
     9061 gaaattatgt cgattataaa gcagttttat tccaaaacga tgacaaaaac caaagaagtt
     9121 gattgcaagt ttttatcgct gatcaaaaat tccagtcatg taatagctat ggatgcgacg
     9181 ctgactcggc atgtggtgga gttttttgcc gcgttcaagc cagatactca aatcgccctc
     9241 atcagaaata catttgtgtc cgcgatgttt tccaataggg tcgcttactt ttgtgacacg
     9301 ttttttggta aagagttttc tttttttgcg cgcttggaag acaagctacg atgggataag
     9361 aaattgtgtt tgttttgcag caccgtgttg gcagcggaat acatgcatga tttgatacgt
     9421 tctcggtttt cactcaaaaa agttctcttg ttgacgtcta aacaagggaa atgctcttcc
     9481 atagaatcgt ggatacggta tgacgtagtg atttatacgt ccgtcgtgac tgtcggtctg
     9541 agtttcgaac cggtttattt cagctctttg tttgtttaca tt
//
