LOCUS       sequence-172645-        8932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-812
FEATURES             Location/Qualifiers
     source          1..8932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4360..4380)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4360..4378)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4399..4415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4399..4415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4412..4434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4423..4439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4447..4453,4454..4471,4472..4477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4492..4513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4630..4647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4801..5389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4881..4900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5560..6351,6352..6420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6183..6202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6421..6525)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6552..7007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6639..6658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6849..6870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7133..7155
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7147..7164
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7148..7164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7174..7193
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7174..7192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7225..7241
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7225..7241
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tagcgcgatc tgtgcctgta gttctggaaa agatcgagtc aatcatagag aaaatttttc
       61 aaacgtctgg gccaaatatc gttcacgata aagacagggc taaaattgcc ttgtgtcgtc
      121 tgttgttagg accggttgcg gtgccatgtt tctgtgaaga atgggacacc aacgactatt
      181 tatcgaaatc tggatgtaaa tgcttaggtc cgatcttata tattcatacc agtcgatgtc
      241 gctgtagcga tattccggtt tttaagtttt ccattatgaa agattactac gcttcacacg
      301 tgttcagagg tttattatct ctgaaagagt ggaatacaca tctaccgaat gtattgtgta
      361 cgtgcgagtt gtcgatgagt gatagatatg tggcgaccgt gtttcctaag cagaattcta
      421 tatatctaga atactatccg tattttttgt gttatctgtg tcgctatctt actgtcattg
      481 agattgagca gtgtacaaat gatttaattt cgcttcttgg tcctaaagta gctcagcgag
      541 tcataattca ttttaaactg cttttcggtt ttcgtcacaa gccgcatatg ggcactgttg
      601 attcgtggtt ctgggaaaat ttttttatgc tagaattgca taagctttgg ttaaccgtag
      661 tcaaacataa ccgggtgaca acagatttct ttaatgtagt ctatgagaaa attcaaaact
      721 ataaacaata cgccatcaag actttgagga tgtcgtctaa ggcggttcct gcaatacaga
      781 ggttttgttt ggcaaaattt aagcagcaac tcttgtatct aaatattaag gttacggtaa
      841 aaaaaaataa gcgagaactg tgtttaaatg gttttgttta cggtaaaaca ttgtatgtcg
      901 ttgaatcttc tcagttaatc tttcgaaatt tgcttctgtt gtattacgat tacagtttgc
      961 cggacgaatg caaaacaaaa gaagaaaacg ttttgacgac tcattacata cgggtaattt
     1021 cgagattgtc gtttaagcag tctcgaagtg cggtcccgcc aggcgtgaaa ccagatttta
     1081 ccttcgtggc acaactgcct aaacgtaaag agttgcctaa tgttcctggt ggtattgatt
     1141 ttgctgaaat tacctcagtg aggcatggcg cggtaattct taacgcgttt aatacgaaca
     1201 aagtcatgaa tttaaaagca accatttcaa aaagggctaa ttttgtgtat catcgcattc
     1261 ctaagacaat gactcacagt tttgtcatgt acaagcatac atttaaagaa cctgcgttta
     1321 ccgtaagcac gtttgtttca aacgatgatt tagatatgag ttcattgaat atcaatatac
     1381 gtggacctta ctgcgacttt ttatatgcct taggcgttta taagatgcat gtttctatcc
     1441 aagatctgtt tttgccggcg ttcgtttgca atagcaataa ttcagtggat ttacagggac
     1501 tggaagatca ggatgttgtg agaaatagaa agaaaaaggt gtattggatt actaactttc
     1561 cgtgcatgat ttctaatgcc aacaaagtga atgtgggatg gtttaaagca ggaacaggta
     1621 ttattcctcg ggtgtctgga aaagaccttc aaaatgtttt gcttcaggaa ttaaataacg
     1681 ttcgagagat tccagggtta gtctttgata tggatttgca tcaattgctt gttttgctgg
     1741 aacagcggaa tctacatcag attccgtttc tcgttaaaca gtttcttatt tttttacgtc
     1801 ttggcctgtt aatgggttac ggaaactctc ggcgcaacaa ggtgcatgat atcatgttac
     1861 acttaatttc gaatggcctg tttgatttta ataagaactc cgtagcaaat acaaaaatca
     1921 aacacgggtg tgctttggtt gggacgcggc tcgccaacaa tgttccgaaa atcattgcta
     1981 ggcagaagaa aatgaagcta gatcacatgg gacgaaatgc taattcgctt gccgtgttgc
     2041 gttttatcgt taaaagtggg gaacataaaa ataaaactgt tttcattaaa ctgttggaat
     2101 atttagcgga aacctcaact gccataaata cgcggaatga agtcgccaga ttacttctga
     2161 ctctgacgac taatatgaaa acatgaatgt acccatggcc gacgaatggt tcgattgcgc
     2221 gattaggtta gattcggaaa ccatagctgt ccatgaaatt ttcaattcgg atttaagtaa
     2281 actgcttaac ttgcactcga aaacagtcta tatgtccgat ctgtgcgcct ttatttctgg
     2341 ttgtgttaat cggaatgttg gtaaacttac catatattgg cacgtgtacg gagacataat
     2401 ctacgcattg acgggtattt tacattgtgt aaaaataacg atagagtgcg gggagagaat
     2461 tgccgatggt cgatatagat tatacgaaat tccgaaatta tttttaatga gaggacagtc
     2521 aacacccctg gaactgaagt ggaagcacgc cgtgggtatc gcgacgacga ataagccttt
     2581 gctgacgcac gttttaacag atgtgttgga aacctctcct tttaccttgc cagatacgct
     2641 tctgtcggtg caggagttgt ctattttcag agagagattg tcgtatattt actatgtgct
     2701 cgggttagat gttgatatcg tagcgagaac agagagggag atttttcaaa aatgtgcaga
     2761 actagctcgc ctacaacaag tctttcttat tcaaggaaat attatggaaa actttgtcct
     2821 cgttcaggcg tgtctatttc agctgggggc tgatggtttg tgggaagaga tgtctggctc
     2881 tgtacgtcct aggccggagt tgatgtctag tgcgttcatt caacacagag taatggtgaa
     2941 taattgttat tgtctcgctg tcatcttcaa tgccatttat aaacacaaag tttccctgcc
     3001 taccgtagaa agaagccacg aaatcgttca tcgtgtagtt caggaatatt ataagtctta
     3061 tgtgaatgct cctctctctg ttcttgtgtg tgcgactaag gtgcttacct tgtttacaga
     3121 agaatataac tttcagtcag ctctcgtatt tgtcagtcag tttttccagg tggacgtcga
     3181 ggcttcgaga gcggatgtga ttcgtctgtt tttagcgtgt ctgaagggtg attagatctc
     3241 tcggaaagag gctgaactgt ttccagagca cacataaatc gccataatta tggcgataat
     3301 taagtcgtca gaacaggctt gttttttagc gctgtatgtt atgtaattgt taacggagat
     3361 ctggtgaatg tttctgattt gttctagtgc gtattctacg ggatcgtagg tgatttttat
     3421 tgtaaacgat atcaattcct gtgatgcctt gatgtttcct gaattaaaat tcgagatgaa
     3481 aaactcaact gctagttttt tttctttacc gagtaggtaa aatggctgtg ctatctgatt
     3541 ctggtctggg gtgtggaaaa aagtcacctg tatagattta ttagccgtga tgttttcctt
     3601 tatgatacat gcgattttta cggcagaagc ttgattggaa ttcccttcta tgatgatttt
     3661 tacttccgtg aagaaaggat gtaggtctag gattgacaat atcatatgcg cggcacattc
     3721 agctattgct gtgtcggaac tcgtcattaa actttctaga aagtagtgct ccatgccgta
     3781 gacgatatat tgatctagat aggtgcctat cgctgctatg ccagtgccag aagcacggcg
     3841 gttgcctgta taggcaggat ctaggtatac gtacagatct ttacctaaaa aaggaattag
     3901 atttttattg atggtgctgt atcggaaaaa ctcgaattcg gtttgtcctt gttccgtaat
     3961 taaaacgtcg ttgatcacat tgcaagtggc tccgcccatg atttcatgga tgaaagctcc
     4021 ttctaggaag aggttggccg tttttttaac ttcggcgtta atgctgatga acttgggttt
     4081 gtggagtcgg tagcaagaac aggctgtggc gttaccgcgt tcgtttagca tatgggcgtg
     4141 atcttcgcat acgtaagaaa ctacggagag catttcaaac ggagagttgt tcagcttcat
     4201 taaaaaagaa gttgaatggt ttccggaatt ggtcgaagat ataaatagga tcttggtaga
     4261 tgcttggggc aggaaaccta aaatcgtgct gaacgcgtct ttctttataa agtggctctc
     4321 gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     4381 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6541 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6901 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6961 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
     7021 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7081 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7141 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     7201 gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc
     7261 gatatcaagc ttatcgatac cgtcgacttt cgtataagtt gtataaggtc tgtaagatcc
     7321 tggttgggtt ttctcgggta attttcagtt gacatagttg agaaaagcgg tggccggtag
     7381 gcaatggttc gtttttcatg agtcgttcga cgatggtcga gtcgtggaca atgggatggc
     7441 aaaccgacgg cagtatgtcg gcaaaatcaa ttcgctgcac gacttggtct ttattgtgga
     7501 aaaaaacgct ggtgggtagg ttgttttcca tggtgtcgtt taatttcaca cgtgtttcca
     7561 ttgtactgaa accggtttct gtcggtatgt agatacccaa agggaaaaag aatgtcagtt
     7621 gtaagctttg ttctagcgga tcgttgacgt ctgtgttttt gtaaactttg tgtaggtggt
     7681 cgaatgcgac taatttatct cccagtttta acgtgttcag ttttaggtcg gcgtacgcgc
     7741 ggcttttgtc aaagatttct gatttgttgt tagtgtcttg ttgttctgtc ccggcgttca
     7801 cggtgaattg agtaaaatcg gccatgattg cacggtatgc aatcgcagtc actgcgtttt
     7861 ctttgcccat aataaagtga ccatatgaga tgggggcgga gacgacctgt gtggagatgt
     7921 actgggagag tatgttggtg agtttttgga tagagccgat cggtcctaat aaaacaccgt
     7981 ctagggggat gttttctttg gaagtgtagt tgttcgtatc atttagaatg ctttccgtga
     8041 ccgattccat catgtcgttc agaattctat agatatacga actattatta ttcctgttca
     8101 agaaaaataa tgacgttaac aatctgtttt ttaggctctg aaacatgttg ctgcgctgta
     8161 ctcggctcag agcttgttta ttatgtactt tgttctcatt catggtcagg acgataaatt
     8221 gcgggggaga ttttctcaat aaggtttgca tgaatgcgtg aatcagacct cgttcgagcg
     8281 aatcggctga attctttaaa gatcttaaaa ctgtatgcaa cgcattgatg tttaaaatct
     8341 gatcgatgac tgtgttttta aatgtgtttt ccagatgctc cagacaggca gcactcaaat
     8401 cgaacgatat gtttatgggg tgtttttctg agtgtttggc taccatgatg gtggtttctt
     8461 ttggagcagt tacgtcgttt cctgtggcga ctctgggtaa ttggataaag aagagaacct
     8521 tcccgagggt cattcgactc acatcgttga agcgaattac gttcgccgca acggcgatcg
     8581 gcgtggtgaa aaagttgatc cattctattt tgttgcagta gattccgagc aaggcttcaa
     8641 aactgatgtt gtaacggctc ggatcgtcac cgaaataaat tcgcaaattg tcaaaaagtt
     8701 gttcggccgt gtatgtttta atgtcattga aaatatttag aggtgcttcg attttaggta
     8761 aaatttcggt cgcctgccaa ttttccatgt ttcggcgtta tgcagcacac cggtaattgt
     8821 gaaactttaa tagttaattc atgttttggt tccacttgtg cacggtcgat tccagttttt
     8881 atagattcct gtgatctgac cgctgaagtg tctcgcgacg aggaaactcg tt
//
