LOCUS       sequence-172646-        6660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-809
FEATURES             Location/Qualifiers
     source          1..6660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2551..2567)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2551..2567)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2599..2618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2600..2618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2628..2645)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2628..2644)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2637..2659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2785..3240)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2922..2943)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3134..3153
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3267..3371
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3372..3440,3441..4232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3590..3609)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4403..4991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4892..4911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5145..5162
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5279..5300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5315..5320,5321..5338,5339..5345)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5353..5369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5358..5380
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5377..5393
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5377..5393
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5412..5432
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5414..5432
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtttgctccc catcctgtgt aaattgtttc aggccagcat cgattaattc cttagtatta
       61 gaaaatcgta acgttgtacc ccatgatgta aaaacataat aaaaaagcgt ttcacttaac
      121 gcaggcagaa aaaatcctcg ttcctgaata aattctgtac aggaagataa agaaatcgtt
      181 tcttcagttt caaataaagt tgcagagtat agaggaattt tcacacacga atactcccca
      241 acttgtactt gaatttgttc ttcaacacaa ggaattaaca gaccagcgaa caacaactct
      301 ttgaaaccgt ttccacacgc aacatggcaa ataacagaat ctgcatcttt gccgacagca
      361 gaacaaagag ctttgatatc gattactttg cgtgctgtag gcacctcaaa cgactgcaag
      421 tgaaatttct gtctggtttg ctcacataat cgaataagat caccatgatc ttcggccata
      481 ccaactaact gctcagttcc atggaaaaaa aaacacacgg gcataaaaga cgtaatttta
      541 gttagaattg tgcctccaaa gttagtgata ggtgttttaa ccgtggtcga gaaatcgttt
      601 tctacagtca aattcattaa caacggcgaa ataactacag gagaatttct ctccattaac
      661 gataaaacag ataaaacatc caaaagttct ttctcttttg gaaaaacata gatccaagca
      721 gcagtagaaa caggagcaga aaccaccgtt tcgttttcat cagccatctt tgtggattcg
      781 atcacaaaaa caaagataat gggtttttgt ggtgaaatcc ttatatatta tgtttgacgt
      841 aacataacac gcgtcatcaa acataaaagt aaaccacaag ttgaaatata ccgttttcta
      901 tatgagtttt acggacaaaa gaaaaacgat tttcttatgc aaatattttc cacgcagatg
      961 acatgacacg ccccttaatt taaatttatg taaatcgtcg tccacctcag gtacaatagt
     1021 atattatata tatagttttt taataaactt attgaggacg ggagaacgaa ggcgtggcgt
     1081 ttacgtcata gcctaattat acattctcag aacaggattt aaaaaggctg cgagcggccg
     1141 gctgttcaga gggacgctgg ggtacgactt gagacgtttg actgaaaatg atccttcgtg
     1201 tactattttc tacaaaaaaa ttaatttgcc ggcgacagta aacttttcag cggaatttca
     1261 aaaattaatt ccacatgtaa tttaagcatt ttaaaacgta taactcacaa ggtgaaaaca
     1321 tatttacgaa tacagtagtt ttcgtgatat tttttaaaaa ttaataaatt ttaaatcgtg
     1381 taagtaataa agcatactat tagattcttc acgttaacag agcaagctct tcgaggtttt
     1441 cagtagaatt tcaatattta attccacatg taatttaagc attttaaaat gtagaattca
     1501 caaagtgaca aaacattcac aaatacagta gttttcacgg tatttttgaa attaataaat
     1561 tttaaatcgg gtaaatggta aagcatgctg ttaaattcac gttaacagag caagttcttc
     1621 gaggtttgct atgtgtttat cagtgaatgt atttagtatt tcaattttag atcatggttc
     1681 ggtaaagata taagtccgtg taaaattttt tggtttagtt ttcatatcta ctagactgtg
     1741 aggtgtttga agtcgatggt ttagctcatg tcaaaacgca atgtcaaatt gtaataagtc
     1801 gttcatttcg tgtttagaaa taaggaattg gtatttagaa tagctttgtc gcatttttag
     1861 ccgcgacgag aaggttatga cgacgttttc gattgcattg tttgaacctt tttcggagtt
     1921 tgaatttacc gtttaaagaa aaacagtttc acgaatagaa tctaaaacag gcttccaaat
     1981 tttagttaat atttatttcg aatttcactt ggggtacaga aagctttggc atcgagattt
     2041 ttttgatttt aaaaagaaat agttcagagt gtgacagcaa aaacctttgt acaaaacata
     2101 cttgtaagaa tttgtttaca gtaagatagt tattctgagt cagaagaata tgtgtttaag
     2161 ttagtttcac aggtgtcagc ttcatagtct aaatctaagt ccatttcaac acttgattca
     2221 ctttcatgtt tagagttgac gttttcatta atgttaataa tcacaatttc gtcttgtttt
     2281 gcttgattga agtttagttc tctaggatct ggtattttgt tgtcggtttc cattgattct
     2341 tctatgagaa tttcggtagt tgtttttgtc gttgttaggt ttgaggtgtc tggtttttcg
     2401 acgcaaactt tggatttaac cttaggcgta ccttggtttt tatgtgaacg atgagaggtc
     2461 tgaggtaggt cgcgacttgt tgaatagctg catgaaggtt ccatgtcgac ggtatcgata
     2521 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     2581 cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     2641 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2701 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2761 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     2821 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2881 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2941 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3001 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3061 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3121 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3181 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3241 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3301 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3361 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3421 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3481 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3541 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3601 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3661 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3721 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3781 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3841 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3901 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3961 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4021 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4081 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4141 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4201 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4261 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4321 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4381 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4441 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4501 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4561 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4621 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4681 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4741 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4801 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4861 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4921 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4981 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5041 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5101 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5161 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5221 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5281 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5341 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5401 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc
     5461 tcgaggtcga caacatgacc tgaagccact atcaatgcga ggttctgcaa aaccgttaaa
     5521 atgaccttat aatatgcaaa cgtcaaaaaa ggagaaaatg cagttgcata aggggtcgta
     5581 tctacattaa atgattgtag acaattttct atctgttctc taggcgtttg tgtccttctc
     5641 atttcaacaa tgcatctaga aacacattcc tcgatacatt gtgtcaatcc gtggattata
     5701 gaaacaaaat cttttttact tcggacattg aaagtatctt cgcctgttac aggatcaatc
     5761 agagaatttt ttttacagta atcgaagatc tgagatacaa atttacccct gtccagtgag
     5821 agcgtgtttt gttgatcttt tccaatctct ttgagctcgc tgacaggctt cctgccaaaa
     5881 gaacctaata tatcaacctc ggcataatac cgagaaaaca tgctcataac aagtggttcc
     5941 tttttaacat tttttggtat tgccggcaaa cgagtaccaa cccgcaccat ggcagttcca
     6001 atacacgatt gacaacattt cccatcacag aattcacata aattcgccga acaatttacc
     6061 agatgattgt aaatttcagt gtacgcatta cccgcgttat aaacgctcat acgatttaaa
     6121 ttccacacta cataagaaag cagctgtggg caagtcgcac aggcataagc caagtgatta
     6181 gctgaaaatt tgtcatcttt gcctgatgtc gaatcacatt tgatgatttt tctattatcg
     6241 ttatacacat cctcagtcaa agacgacaac gcattacaaa aagacaaaga tcgcagcagt
     6301 aattcttgag tcatcaccga attaaacgct tgtttcacgt ttggtataaa tgccaatttt
     6361 gagcaatata agatagaatt tggcgcaaag atcaaaacgg caagatgact agacagatgt
     6421 aactttaaat tcgtgagctg cgccatgcgt tcagcgtgtg tctctgagtt tcttatgatt
     6481 ggccagtcat tgaaattcat tatctgactg gggtcataca ccgaatccag gtattcggct
     6541 acatgactaa acgataattc tgtcacaacg gaatcaacca acatcaaaaa atctttctcg
     6601 accgctgaaa ttttctgtcc ggagataccc agataagttt tgtggggtgc taatttgaca
//
