LOCUS       sequence-172647-        4282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-807
FEATURES             Location/Qualifiers
     source          1..4282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..76
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     95..114
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        95..113
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     146..162
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     146..162
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1563..1583)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1563..1581)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1602..1618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1602..1618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1615..1637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1626..1642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1650..1656,1657..1674,1675..1680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1695..1716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1833..1850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2004..2592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2084..2103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2763..3554,3555..3623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3386..3405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3624..3728)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3755..4210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3842..3861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4052..4073
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
       61 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
      121 ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt
      181 cgatatcaag cttatcgata ccgtcgaccg acgaataccg gcgtgagaaa ttttgtgata
      241 aatttaaccg tcagcgaatc ctcttcgtcc agacgcaccg caaacagaat tttgctgcgg
      301 tctttcacga gcctgttgta agaaaaaaca agttaacgga gagtcttcat cctcgaagaa
      361 ttgtcttttc gggaaaaacg acacacaaca catctaccca cctgtccttt cgcacgcaca
      421 tatgccgagg cttactgcgg agaacgggca actccccatt caacatccta ttaagattct
      481 ccgtctcagt gtcacttata taaaataagg tatcgtcatt ccagtccaat gaatacttct
      541 ctttatagag caattcatca aatcctctac gtataaaagt cctcaaactt ttagagactt
      601 tgctgattct gttttctacg tagatataga tgtgacagtt cgagcccata acgatagcag
      661 ctctgcaatt tggttcagct tgagagcggg cacacgtccc tatgatagca taatcctttt
      721 ctgtgatttt ctctagaatg ccaggtccag tcagataata aatatccgac aaggaacaaa
      781 aggaaaagtc aaagtcctgg aaagcatcca ccctgattct cttgccacaa tgttttgcta
      841 cgtaatcctt catagccggg agattcctct gtaaaaggat aaattcctct cggccgcatt
      901 tagtctctcc gaaccacatc ttttcctgct ctggatctaa ctccgcttcg gcaaagggtg
      961 gaaattgtct aagtccgata ttgaagaact ggaccagtga ttctgctatg atgtacactt
     1021 tctccgtcac cgtgtccacg gcaatggtct ttccagttga actatgcatc acgtatggat
     1081 cgtagtcagg attcggatca cgatatatcg ggtgagcgta cccaatggcg gacaaaaatt
     1141 catcagtatc cagaagactg ctccagtcat aggcacttac ccatctatgc gtgtgaaagt
     1201 gataaaactc acccagagtt aatgcataca tgggaggcca ggcgatacaa catcgcaagc
     1261 ccggagaact gatctccaac ccgtctccgt aagctgcaga cagcacgtct acatcaaaat
     1321 ccaaagtagg cacacatgat gacaaggtga actcatcgtt gcagaccggt acgatttctg
     1381 tttgaaacat aaacgcaaaa tgaagtacaa tactttcaca caagactcgc tttccgccct
     1441 ccgtttcaag tatcagacga aaattcgaca tgaactactt actgaggctc atgcacgatt
     1501 gataagtctc cgacatgccc aacgtcgacc tcgagggggg gcccggtacc cagcttttgt
     1561 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     1621 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1681 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2161 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2401 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2821 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3721 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     3781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3841 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3901 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3961 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4021 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4081 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4141 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4201 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4261 cctcttcgct attacgccag ct
//
