LOCUS       sequence-172649-        4399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-805
FEATURES             Location/Qualifiers
     source          1..4399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     52..68
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     52..68
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1586..1606)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1586..1604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1625..1641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1625..1641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1638..1660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1649..1665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1673..1679,1680..1697,1698..1703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1718..1739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1856..1873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2027..2615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2107..2126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2786..3577,3578..3646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3409..3428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3647..3751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3778..4233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3865..3884)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4075..4096
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4359..4381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4373..4390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4374..4390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg ctctagaact
       61 agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgaccgcgag
      121 gagagccttc tctttatagc tcgcaatatg tcagattttt taaaaattgg atgtctgcgt
      181 tataatgaga atcgtcggtt gcatacgcaa tggtttacgc aagatacgga ctatattaag
      241 caggtggatg agatgttttc tagagacgtc ctctgtcctc tccgtgagca ctgtcagagg
      301 tcgcgccggg atcgaggatt attaaagata tgtacgtctc tagtcagggg aattaattgt
      361 attgaaagag ggtaggttgt attgttttac catgttgctt ataagcaaaa ctctattccc
      421 cattcctaga agtgccgagg aactctggga aggaacatac actctcgtag tcgcatttgt
      481 gctcgcattt ctcgtctact ccgattatct tagtaattta agtccgtttg gagaaatttt
      541 gtcctcgccg tgtatttcaa cgtgaatctt cgtcgggaag aggatggatc gccctcggac
      601 gccgccgccg tcctattcag aggtgctgat gatggacgtc atgtacggac aggtttctcc
      661 gcacgctagc aatgatacat cgtttgttga atgcttaccg cctccgcagt catctcgctc
      721 agcgtggaat ttgtggaaca agagacgaaa gacgtttgct tttttagttt tgacgggctt
      781 ggctatcgca atgattttgt ttattgcttt tgttatttac gttttcaatg tgaatcgtcg
      841 aaaaaaatga attacctgtc ttagaagcat ttgctgaccc gatgtggaac ggaaacatga
      901 acgttgaaaa atctctattg tttaaaagga atagcaggca tcgactatta aactaacatg
      961 acaaatctga atgtatgagg aagtctgaat ttaatgcaaa atcgtgtttt cttatgatcg
     1021 gcatttgtgt ttttaatttg aattcgtcga gttgtattat aaaaactaat gcggaacatt
     1081 gttctaaaag attgttttca ttctgctatc ttggtgtggg tgtgattaaa aaaccgttgc
     1141 acttgggtaa tcgcaaaaac atgttgttct tgtctttttt actcgtgtgt ctctgtgaag
     1201 aggtgcaaat gctcaaccta acgactacag aagtttcggc aactgaattt gcctcaattg
     1261 cgtctaaaaa tatggaaacg aatgtatcga catccagcga ctatttaact gggaaatctg
     1321 aaactacgtt ttcggcgaat cccgaaacgt ggggtaaaaa tgttaccgaa atatcaatag
     1381 cttctgtggc atatttaaac cagtcttcta tggtgacctc tacgctagcc gtgggaacca
     1441 caaatcgatc cagtggcaat aatgtaaatg ttacaacaag ttcgtttcca acggtcaagg
     1501 gtgatgaggc gcaggatatt gagacgtttt tcacagtaat cttagcgtcg acctcgaggg
     1561 ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     1621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3781 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3841 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3901 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3961 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4021 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4081 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4141 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4201 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
     4261 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4321 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4381 gacggccagt gagcgcgcg
//
