LOCUS       sequence-172650-        5467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-711
FEATURES             Location/Qualifiers
     source          1..5467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..149
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     58..74
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     59..75
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(109..125)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(109..125)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(162..182)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(162..180)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(201..217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(201..217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(214..236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    225..241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(249..255,256..273,274..279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    294..315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(432..449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(603..1191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(683..702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1362..2153,2154..2222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1985..2004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2223..2327)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2353..2808)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2490..2511)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2702..2721
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2935..2957
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2949..2966
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2950..2966
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2976..2995
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2976..2994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcatacgtct gtggcgagga acccatgctg cctttggaca cggtaccggg ccccccctcg
       61 aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
      121 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
      181 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      241 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      301 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      361 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2341 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2401 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2461 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2521 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2581 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2641 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2701 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2761 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     2821 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     2881 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     2941 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
     3001 tgggtaccca aggaactatc tgtccttttg atttcttgcc gtaaaacgtt cgctgcggaa
     3061 attttgcacc gttttactct caatgggttg gaggattttg aattatattg cgatattacg
     3121 gagcggcaga taaacaacag aaaagtcatt gtccagatag aaagcttgca tcgtcttaca
     3181 gaaaattacg atgttttaat attagatgaa attatgtcga ttataaagca gttttattcc
     3241 aaaacgatga caaaaaccaa agaagttgat tgcaagtttt tatcgctgat caaaaattcc
     3301 agtcatgtaa tagctatgga tgcgacgctg actcggcatg tggtggagtt ttttgccgcg
     3361 ttcaagccag atactcaaat cgccctcatc agaaatacat ttgtgtccgc gatgttttcc
     3421 aatagggtcg cttacttttg tgacacgttt tttggtaaag agttttcttt ttttgcgcgc
     3481 ttggaagaca agctacgatg ggataagaaa ttgtgtttgt tttgcagcac cgtgttggca
     3541 gcggaataca tgcatgattt gatacgttct cggttttcac tcaaaaaagt tctcttgttg
     3601 acgtctaaac aagggaaatg ctcttccata gaatcgtgga tacggtatga cgtagtgatt
     3661 tatacgtccg tcgtgactgt cggtctgagt ttcgaaccgg tttatttcag ctctttgttt
     3721 gtttacattc agttggccaa aggagggccg gacatggttt caatatttca atccattggt
     3781 cgtgtgaggc gagttatcga tgaagatata tacatttaca tgaatcccgt actaattaaa
     3841 agctatgatc ctttggcgcc tattgccatg cctccttgca gtgattggag tgtcgctgag
     3901 cagtctatca tctcagagag ttgcattgac ttccgcggaa agtgcagcgg agcgcataaa
     3961 tataatttct gttccgtact gaagtgtctt tttcgataca ggcattatat cgaaaagact
     4021 actattacaa gtttatctga tagtctcttt ctgctctgca gtctgttatg tgagaattct
     4081 attaaggtgg acattgttgg aaatggattc ccgatgcgca aagaggtttt tttgagtttc
     4141 ttgcaaattc tagtcgagga gtgtcatttt atcgaaaaga agattacttt gccgggtgac
     4201 aacatgacgt tccaagagat cataagtagc agagaaacta tcatgaacgg agatttttat
     4261 gaaaacggta atcagctatt acataaggac tatattaccg acatgggaaa gttcagagcg
     4321 acttttttat ctccgggcgt tgacattttc attgcatcgg acattgtatc tgatctgaaa
     4381 aatgaaagca aacgctatgt ttttgtgaac gtctggttac aaaaatgtgt gtctgctggt
     4441 gtagaaagca cgcgaatcga aagagttttt aatgagcgga ttaagtctta tgtgctgccg
     4501 aagagttttt tgtgcgatga gtactttgtt ctaggtgaca tctctggggt ttacgagtgg
     4561 ggaatgttaa tagatctggc cttcctggcg gaaatgatcc gaaaagattt gaaactgaaa
     4621 tcgtgcacag atactactac tgacatttct gaagatgatc tccttttgtg tgcggccagg
     4681 aggtcgagtg acatcttaca aataatgcaa ttagtgttta cggtgcatgt gcaatttttt
     4741 caaagataca gtctgcagac tctgcagctt tttaacaagt tgagaggcat gcggattgtg
     4801 acgggtgtgt tttctataga aaaattcagc atttccatcc tcaggctttt ctttaaatgt
     4861 gcatttaata tgactttgtc cgccagcaaa cctcggtaca tcccgggaaa ggcgtatcgc
     4921 aatctaacaa aaaatgatct ggagaacatg ctagacaatt gggagatttc gcgcaccaat
     4981 cttaagactt gtaaagaact acgtaaagcc ctcaccgagg cctcgagagc aaggcggaaa
     5041 caaacaattt ataaactgca aggctcggat ataagccttt ccgttagtga agttggagtt
     5101 tttgggcaac atgcatctcc gggggtgtgc gtgtcatctt agcctttatt gtgtgtacaa
     5161 cgactgggag aataagatct acagagtacc gatttttcag tgtttgtttt tagaagcgga
     5221 gactcgttcg ttaaaaacgt ttttgattag gggacagagc ctagaccaag aatcgttaaa
     5281 tgagatagaa gttacaagaa aagaaacaat gctttgggac cttcaggaac aaagtaatat
     5341 gatggacaaa aaaatagcag caatcagcag cctcattatg aataacggag agcttcttag
     5401 gaaactttca aaatttttcg tgcctctaac tgttgttctt ggagacgacg ggttagagat
     5461 tttggaa
//
