LOCUS       sequence-172651-        7357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-710
FEATURES             Location/Qualifiers
     source          1..7357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    706..813
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     722..738
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     723..739
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(773..789)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(773..789)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(826..846)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(865..881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(865..881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(878..900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    889..905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(913..919,920..937,938..943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    958..979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1096..1113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1267..1855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1347..1366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2026..2817,2818..2886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2649..2668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2887..2991)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3017..3472)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3154..3175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3366..3385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3599..3621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3613..3630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3614..3630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3640..3659
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3640..3658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtccaagcta gcagaagaac agctaatggc gtatagcggc aaattgccta tcctataaat
       61 actgaagtta ataggcgtgg taggaaatga tgattaatac atcattaaaa tattctactt
      121 catggctttt gattcgttat tcgtaagtgg gtacaatgaa aaaaaatcaa cactgcctta
      181 atgtatgcac cctcaagagt gctgattgga tagagatata atgatatata aaaaagtgca
      241 tgatgaacaa aaaagtttgt ttttccataa atttaaatgt cagtgggtgc cgcggttagg
      301 gtctttccta cccaaacggg tgagaacctt aaaaattgct aacggggcaa ccgcagcttc
      361 tgtttttcat caaaaagtta aaggtcagtg ggtgccgcgg ttagggtctt tcctacccaa
      421 gcgggtgaga accttaaaaa ttgctaacgg ggcaaccgca gtttctgttt ttcctcataa
      481 agttaaaggt cagtgggtgc cgcggttagg gtctttccta cccaagcggg tgagaacctt
      541 aaaaattgct aactgggcaa ccgcagtttc tgtttctctt catacagtta aaggtcagtg
      601 ggtgccgcgg ttagggtctt tcctactcaa gtgggtgagg accctaaaat tcgctaacgg
      661 ggcaaccgca gtttctgttt tttctgataa agctaaagat cagtgggtac cgggcccccc
      721 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
      781 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
      841 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3001 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     3061 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3121 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3181 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3241 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3301 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3361 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3421 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     3481 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3541 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3601 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     3661 gaattgggta ccttgctgcg aagctccgat cttcatgacg cagttgtcat tacagctgac
     3721 agaggctgca ctggaaagca catcatcttc ttcagaaaac tctaaatccg caatggatat
     3781 tttaccctcc gatgcattct gctgatgtga ttcctcgaac tgcgctagag aagttccata
     3841 acaaggattg tccatctttg acatgttttc tcccatagac agaagggctg ccaatttgga
     3901 aggaaatcct atattttccc ctcggtcatc atagtcaaag tttaattttt ttattgcacg
     3961 taggctgggg gtaggcttgc atacatcttt attcggggtt ccgttatttc caatcattga
     4021 atgtgcaatt attgtcgggt ttcctccagg cgaggacaac tgaggagcac atccattact
     4081 aaaacttgtg tcctgtatgc tctctctgac ggacaagggg ttgttttcat ccactgtacc
     4141 gagcaaactg tagtgcgctt tgaacgctgt attgggactg cagatctgta ccacgggaat
     4201 tgttccattt ccttcacatg cgccatgttg tggcacagat gtgtagggct gaccactgga
     4261 tagagttagt ggtaaagctg tattgacggc tccgtggaca ttcccatttt ccggaaaacc
     4321 atagttctcc atttttgttt cagcatttat attgtttaac agatttgtgc tcgtcccggt
     4381 cacccgcatc caatcttgat gttccggatt caatagagaa gatgcgacgt tagcggtgcc
     4441 accttgttta tcggaaatgg ccgcatctac acaccccaca tggccataaa cattcaaccc
     4501 gttgcccttc atcagattag gagcgattcc cggtgtcgaa gaaacaactt ttccctggga
     4561 gcagttttga gatacaatgt cttgaacttt acagtattgg ttccatggac tgttattatc
     4621 catataattc tccatgcctc ccattccaaa attagcaccc cgattatttg ataccgcccc
     4681 caccggcttg gaagtagaag tgacaatcgc atccatagta gtttgtaacg catttatgca
     4741 gttcatggat gcgcttagtt gtgtcgcctg tgagttgttc atattctgtg tttcgcacac
     4801 aggagtgaaa cctggcgtat ttacatacgc acatgattgg agagcagccc cctgatagaa
     4861 accgtcgcta gacccagaga cataaggttg ggcatgcgag acctgttgtg ccttgttgac
     4921 aactgaatta gttctgttat tcggaggatt aggggggttc cgtttcagag tccaggattg
     4981 cccgccgggt gtagtcagta tccattcatc gccagatgcc gtagtgatct cataagactt
     5041 agacccgttt acagaagatt gattatggac agactgatat tccccgggtc ttataccggc
     5101 atttcccgta gtaatttggg aagctgaaat agagggtggc cccacagttt tatttgtctg
     5161 atatttaccc tgtgaggtag cggtcatgca tggatgcata cctacaaggg atttaagttg
     5221 aggctgttgg acaggcggtt gagcattaac atacaaggaa tggtctgcaa atgacatctc
     5281 ccccatcgaa tgaccatgtg atttgataat tgatctaggt ttgatgggat tgatcatatt
     5341 attgctatag acataattct gaccctgcat cggcggggaa atgggttgaa aggtcatctg
     5401 agtgggttga acattgattt gattgttgat gctttccagc gagaggcccg tcagagtcga
     5461 cagtgattcg cggttttgat cccgtacgcc attggaaaac tgcgactgtg tcgtcatcac
     5521 tccagtatga cccgccgatg tcgttgattg gactgacatt tcaacgtgcg gagattgcgt
     5581 aaaatgatgt ccagctgcga tgttttcgtc acacgtggac gagattgcat tgtccgacag
     5641 aagttccgcc ggatccatac tgagaatttg ttgccataac aaatcttcca gatttgaaga
     5701 catctagagg tgacaagcca ggggaattac cttattttcc gtatacaact ataaaaatct
     5761 tggaaggcac aaacggtatg ttttccataa taaatccaag tgatgatttt tggactaagg
     5821 acaaatatat catgttgact atcaaaggcc ccgtggagtg ggaggcagaa atccctggaa
     5881 tatctacgga ttttttttgc aaattctcta acgtgcccgt gccacatttt agagatatgc
     5941 actcaccggg agcgcccgat attaaatgga taactgcatg taccaaaatg atcgatgtca
     6001 tactcaatta ctggaataat aaaactgccg tccccacccc tgcaaagtgg tacgctcaag
     6061 cggagaataa agctggcaga ccctccttaa cattattgat agctttagat ggaattccca
     6121 ccgcaacgat aggaaaacac acaacggaaa tcaggggtgt attaattaaa gatttcttcg
     6181 acgggaacgc ccctaaaata gatgattggt gcacgtatgc caaaacaaag aaaaatggtg
     6241 gcggaaccca ggtcttcagt ctaagttata tcccctttgc ccttcttcaa attattagac
     6301 cacagttcca atgggcatgg acaaatatta acgaactggg agacgtatgc gatgaaatac
     6361 atcgaaaaca catcatatcc catttcaata aaaaacctaa tgttaaactt atgctgtttc
     6421 caaaggatgg gaccaacaga atatctttaa aatctaaatt tctgggaacc atcgaatggc
     6481 tgtctgatct tggaatagtc acggaagacg cgtggatacg aagagacgtt agatcataca
     6541 tgcaattatt gacactaaca cacggggacg tgctaattca tagggctcta tctatatcta
     6601 aaaaaagaat aagagcaact agaaaagcta tcgattttat agcgcacata gacactgact
     6661 ttgaaatcta tgaaaacccg gtttaccagt tgttctgtct gcagtctttt gaccctatat
     6721 tagcaggaac catattatat cagtggctaa gccacagaag agggaaaaaa aacaccgtta
     6781 gttttattgg tccacccgga tgtggaaaat cgatgttaac gggagccatt cttgaaaata
     6841 tcccgttaca tggaatatta cacggatctt tgaatactaa aaatttaaga gcttacggac
     6901 aggttttagt cttgtggtgg aaagacataa gtatcaactt tgaaaatttt aatattataa
     6961 aatccctcct tgggggtcaa aaaataatat tcccaattaa tgaaaacgac cacgtacaga
     7021 taggaccgtg tcccatcata gccacatctt gcgttgatat acgctcgatg gtacattcaa
     7081 atatccacaa aataaatcta tcacagaggg tatataattt tacatttgat aaagttatcc
     7141 ctcgcaattt tcctgtaatt cagaaagacg acataaatca atttctgttc tgggccagaa
     7201 accgttctat aaattgtttt attgactaca cggttccaaa aattttataa ctatctattt
     7261 aaaaacatct taacagacta cttttaaaat accgacaaga aaataaaaaa aaagacatca
     7321 cattataaga cgctgataac tcaccctgta ccgctgt
//
