LOCUS       sequence-172652-        7690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-709
FEATURES             Location/Qualifiers
     source          1..7690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(126..714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(206..225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(885..1676,1677..1745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1508..1527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1746..1850)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1876..2331)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2013..2034)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2225..2244
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2458..2480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2472..2489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2473..2489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2499..2518
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2499..2517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7255..7362
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     7271..7287
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7272..7288
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7322..7338)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7322..7338)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7375..7395)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7375..7393)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7414..7430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7414..7430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7427..7449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7438..7454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7462..7468,7469..7486,7487..7492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7507..7528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7645..7662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
       61 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      541 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
      901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
      961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1861 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     1921 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1981 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2041 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2101 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2161 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2221 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2281 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     2341 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2401 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2461 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     2521 aattgggtac ccggcattga ccttattggg atggtaaaca ctggggatgg aaaaccttta
     2581 ctttgacaat gaccattatt gatcacttta tcataatcac tcaaatgtaa gtaattatct
     2641 tcagatgtac agtcatctga ctcgctactc gattcagaat taggttctga tttaatagtt
     2701 ttttctaaca ttgacttatc aataatattg ttaccatcct cttcatcttc actctctgaa
     2761 cttataacaa ttacgtttgt ggtttcaggt aacctatcta gcttaatttt tttagatttg
     2821 gatggtttaa ttggaacatt tttcctttta gctgttctct ttttagattt gcatttttga
     2881 gacacagcca caattttctc agttttgtga tcttccttag aaggagtgac ctctggtggt
     2941 gaatatagag acttgctaaa tggtgtattc tcattagtgt tattcatatg aatgttatta
     3001 gttctgacag ttgaatcaca gtctgcaatg taatcagttt catcattgtt atcgctttca
     3061 ctctcataag gtgctgagtg atcagtttca taaccaaatg ttatcatttc tgatattaaa
     3121 gggttgtcat ggaaaccatc acatagattc actagactct ctacagcttc catttctttg
     3181 tctaatttgc tttctggatt tagagtatcc tgaattgcac catgactttt gacaggagtt
     3241 gctgagttgg taaaggtcat accaggaagc gtttcggtac acttggagtt agaacctttt
     3301 tcctgaatcc attcttttgt ctgtgacctt ttatcggggc tgtaagccaa aatgtcttgt
     3361 tgtccagacc cggtctctac aactactgag tcgggttgag tttcaagtag tttttttatc
     3421 aagaagtgtt taccactttc aaggccagta atggatttaa agttatcagg gccgcccaga
     3481 tctgtcactg aggctgtaca tacacagtta gggcttgttt gttctttccc tatctgtctg
     3541 ctatattcaa tgaatgtttt tgcctctttg gatttccctg gcacatctgg agaaggagtg
     3601 tctggataca tttttcctac atctttttca tgtatgtttg taagtttttg tcttgttggg
     3661 gattcattgt tctttgacgg agtgggaata ttattatcag taggagattg tacttccatg
     3721 tattttaaat agtctacatt gtcatcatct tcatggatag attcgataga agattcctct
     3781 gaatattttc catgttcatc aaaatcagct aactctaata atattgattc tgacgggtct
     3841 atgtttaaat cacagatgga agactctcta tttttattta tagcaatgta gccttccatt
     3901 atatcagagg aagttaaagg tttaatcaaa ccagtatcat cataaattgt ttctatgcat
     3961 tcacatgcgt gggtgatcat attcatcatg gtaattttaa actggtgtct gcgaggtttg
     4021 aggtattgcc atgaaaattc attgctgcat agaacaatac tttggagaac agcaatgcca
     4081 atatttttgg cggcagtaat taaattttta caatttatat gaattttgag gaatgtttta
     4141 tcacagttat ccataaagat ttgattcatc tgcttattag caaattcctg aaacattttc
     4201 atatttttaa catggttttc tattatctca tttctacttt ccagtagttc ctgtttaagt
     4261 aaatttactt gcttacaaaa ttccttattg gagatttcag gattggacat ctctttgttg
     4321 tgtgccatca acaacaatga ggtggcgacc atgttcaatt ttccactatc taatgccaca
     4381 taagctttgg aaaaaatgtt tttgagggct gtaataaatt gattgttcat attagctttc
     4441 aaatccatga gcaaatctct ggattctgat gctatctttt tcactaaccc atcaaattta
     4501 tcacatgcaa aaacaatgaa tcgcggttcg caccaattaa aaaaatccaa gttttttcca
     4561 gacaacattt ttgcataaca tgaaacaaat tgagatgaag cactacttag taaattaata
     4621 tttatcaact gtcctaattg ttctaatacc agcctctcca acatggatct gtgcagcgtt
     4681 aataagccag cggagttaat taaattgtct tccatgttag actgttcctg tttcatggca
     4741 gccttcactg atgcaccaat actttggatg caattgccaa cggactgagc taggatgtaa
     4801 aagaagatat tctaagtatg ctatatgtta catgcttaaa actatgtttt tttcttgttt
     4861 tctatcttgg gacatataat atataattca tgtactcacc aaccaaagtt ttgaattcgt
     4921 cagatgctcc ttcttccaca ttactggaat aggacacatt cttggaagcg atgtcgttgg
     4981 aagactctgg gatgaaaaga tcaaaggctt ccagttctgg aaaaagcagg ctttcaaagg
     5041 acacatcaca cttgagactc tcttccaata tttctttgat ggattcttcc accactggat
     5101 cgggatggta gctgtaagta tgaatatttc ttagtactta gaaaaactcc catatagttt
     5161 taaataagat agatctaata aggactaatc atacttactt ttgcatacgt tcctcattgg
     5221 attccatgtt gtttccagag ggttttccga gaatgaacat agaagtagag gttgtatctt
     5281 ttgctgactc cattgtatat taagatgcaa attcagacac cgcctactgt tctgttagcc
     5341 aatgaaaaaa tatcttctgt cagcagatat aatactatat aaggagatta ccaccacctc
     5401 tttctttgca gagattattc tctgcttgaa aatctgtaac actgatcatg atgggatatg
     5461 aggaaaaagt gtcagctact ggaaagactc gtttaaagat actggcatgt ctgatcgttt
     5521 taatactagc tgcggcaata actatgttaa cgctggaaat tatatcgaac caaaaacgta
     5581 ccactactga tctcgaagct gtgactgtgg cgctgaagca tgtaagcaca tctcttgcca
     5641 actgcactga atccactact tctgtacata ccggttagtg aaagatatgt cttttttatg
     5701 ttaatgtatt gattatatat ttgcatacat tccttaagaa tggaaaaatc acggatggtt
     5761 tgtttttaga ttctgtgacg agccaaccca cgaaaaaaca aagaatcgag gaaaaaaatt
     5821 gaagggaaat ctccaagttg ggttcaggct ttaactacag catctggaat tatcctactg
     5881 ttttgtataa tgatgatatt cattacatgt tcctggacca cagaaaaaga tacagagaag
     5941 agtgaagtgc aatcttatgc ttcttcagta gagactttag actctttaaa tgaggctatt
     6001 ataccgaaaa ctgaaatgaa tgtgtaatgt ctgtattttt ctttacagag atgtacggag
     6061 agtttatatt tggggaaaat acctgactgt tctgcctata tgcgaatgtt aaagtatgta
     6121 taatataaat tcttaccttt taagagtgat tcaaggtgga ggtttctttg gagattgatt
     6181 ccaggtggtg gtttcgggtg caatcaatct ttcttctggg cgggaagaaa atccagcaat
     6241 ccaataattg atgggatgta atcaatgtca caaatctgta agattaaatg tgaacagtat
     6301 aaattctttt gtgcttatca aattacaatt atgcgcatga aaatatcatt aaattgtttt
     6361 aaacattctt aaattcaatt taaaataatt tttgtagcaa ttttaaatat taatagttaa
     6421 aaaattgact taccagactg cagcgatata agaaagaatc tctgtgatga gaatgttctc
     6481 cggagccact tcttgagaaa agtggcactg aactggctgt aacttctgct tatatacaat
     6541 tctatagcgg gtgtgatgta caggaaaatg acgaggatga aggagccaat cgctgtataa
     6601 tgttaaaccg caagtgtaga aatacacccc ttttagacaa atatggaatg gaaattagct
     6661 gatgcttaca aaaatttagg gttttgtcat atcttactca aaaacaggaa tttgtggtct
     6721 ggattttttg aggttttctt cttatggctg acttgtattt ttttatgaga aagacggagt
     6781 ttgcggttaa aggcgggata ataaagaaca ctttactgat aaattgtcat gttttttttt
     6841 aaaaaaaaac atctgctaca catcatgaaa caggaatatg gttttggtgt tagcgcttct
     6901 cacgaaataa atgactcagt caggggaaaa tgttgtaacc gcggtacaca ctgaccttta
     6961 actttatgag aaaaacagga actgcggttg ctctgttagc agattttagg gttctcgtta
     7021 gcctgggtag gaaagacccc aaccacagta cccactaacc tttaacttta tgagaaaaac
     7081 aggaactgcg gttgcttcgt tagcagtttt tagagtttta attcgcatga acaggaaaga
     7141 ctccaaccgc aatatctgct aatttttaac tttataagaa aaacaggaac tgcggttgcc
     7201 gcgttagcag cttttagagt tctcattcgc ctgggcagga aatacttcaa ccgcggtacc
     7261 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
     7321 ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     7381 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7441 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7501 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7561 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7621 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7681 gcggcgagcg
//
