LOCUS       sequence-17276-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Avalos JL, Celic I, Muhammad S, Cosgrove MS, Boeke JD, Wolberger C
  TITLE     Structure of a Sir2 enzyme bound to an acetylated p53 peptide.
  JOURNAL   Mol Cell 2002 Sep;10(3):523-35.
  PUBMED    12408821
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-09"; volume: "10(3)"; pages: "523-35"
COMMENT     Sequence Label: pProEX-Af2Sir2
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 acannngnac tccatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatgga ggatgagata aggaaagcgg cagagattct tgccaaatcg
      121 aagcacgcgg ttgttttcac aggtgcagga atttcggccg agagtggtat tcccacgttc
      181 aggggcgagg atgggttgtg gagaaagtac gaccctgagg aggtcgcctc aatttcggga
      241 ttcaagagaa acccaagggc cttttgggag ttttctatgg aaatgaagga taagctgttt
      301 gcagagccaa atcccgctca ctacgccata gcggagctgg aaaggatggg gattgtgaag
      361 gccgtcatca ctcagaacat agacatgctg catcagaggg cggggagcag gagagttctg
      421 gagcttcacg gaagtatgga caagctcgac tgcctcgatt gccacgaaac atacgactgg
      481 agcgagtttg tagaggattt caacaaaggc gaaatcccgc gctgcaggaa atgcggcagc
      541 tactacgtca agccgagagt ggtgcttttt ggtgccatan nnnnnnnana tcnnnnntat
      601 tnnaagcntt tgaagaggca aancnctgcg atgcatttat ggtggttgnc tctagcctcg
      661 tggtttatcc cntgtgactc cctcatgcga gaagtgacga agacaaaatn ntctcgtaat
      721 gcgganccac atgctgaccc ntttgacntn antctcgaag gcngaaattt tcnaaatcta
      781 aagagtcann gtgagatgaa atacgccttc ancaactgat ncagctggnc actgtntgcg
      841 agaaattccn gaatacaaca acgngnanat cgnaa
//
