LOCUS       sequence-172778-       10747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing TIAM1-IRES-RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 10747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs3-TIAM1
FEATURES             Location/Qualifiers
     source          1..10747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..563
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(155..172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     382..401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             575..1270
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     LTR             1439..1908
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2038..2055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2209..2797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2289..2308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2961..3752,3753..3821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3584..3603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3822..3926)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3956..4549
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4612..4969
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5028..5444
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5374..5396
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5412..5428
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5454..5828
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5766..5782
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5857..5866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10672..10737
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
       61 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
      121 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
      181 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
      241 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
      301 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
      361 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
      421 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
      481 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      541 ctttgaaaaa cacgatgata atatggccac aaccatgagc gagctgatca aggagaacat
      601 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      661 gggcgaaggc aagccctacg agggcaccca gaccatgaag atcaaggtgg tcgagggcgg
      721 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaagcctt
      781 catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac
      841 atgggagaga atcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      901 cttccagaac ggctgcatca tctacaacgt caagatcaac ggggtgaact tcccatccaa
      961 cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc
     1021 cgctgacggc ggcctgagag gccacagcca gatggccctg aagctcgtgg gcgggggcta
     1081 cctgcactgc tccttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     1141 gcccggcttc cacttcgtgg accacagact ggaaagaatc aaggaggccg acaaagagac
     1201 ctacgtcgag cagcacgaga tggctgtggc caagtactgc gacctcccta gcaaactggg
     1261 gcacagataa gtacgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     1321 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     1381 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
     1441 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     1501 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     1561 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
     1621 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     1681 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     1741 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     1801 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     1861 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt
     1921 atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta
     1981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2881 aaaaaggatc ttcacctaga tccttttgcg gccggccgca aatcaatcta aagtatatat
     2941 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3001 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3061 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcataatga
     3961 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4021 aaaatacata actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg
     4081 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4141 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4201 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4261 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4321 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4381 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
     4441 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4501 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     4561 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
     4621 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
     4681 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
     4741 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
     4801 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc ccctaatagg
     4861 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
     4921 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
     4981 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta
     5041 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     5101 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     5161 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     5221 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     5281 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     5341 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     5401 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg
     5461 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag
     5521 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag
     5581 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc
     5641 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc
     5701 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca
     5761 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct
     5821 ctagactgcc ggatctagct agttaattaa ggatccgcca ccatgggaaa cgcagaaagt
     5881 caacatgtag agcacgagtt ttatggagaa aagcatgcca gcctggggcg caagcacact
     5941 tcccgctccc tgcgcctctc gcacaagacg cggaggacca ggcacgcttc ctcggggaag
     6001 gtgatccaca ggaactccga agtgagcacc cgatccagca gcacccccag catcccccag
     6061 tccctggctg aaaatggcct ggagcccttc tcccaagatg gtaccctaga agacttcggg
     6121 agccccatct gggtggaccg agtggacatg ggcttgagac ctgtgtctta cactgactct
     6181 tctgtcactc ccagcgtaga cagcagcatc gtcctcacag cagcctctgt gcagagcatg
     6241 ccagacactg aggagagcag gctttacggg gatgacgcta catatttggc tgagggaggc
     6301 aggaggcagc attcctatac atccaatggg cccactttca tggagacggc gagctttaag
     6361 aagaaacgct ccaaatctgc agacatctgg cgggaggaca gcctggaatt ctcactctct
     6421 gatctgagcc aagaacattt aacaagcaac gaagaaatct tgggttccgc cgaagagaag
     6481 gactgcgagg aggctcgggg gatggaaacg cgggcgagtc cgcggcagct cagcacctgt
     6541 cagagagcca attccttggg tgacttgtat gctcagaaaa actctggagt gacagcaaac
     6601 ggggggccgg ggagcaaatt tgcaggctac tgtcggaatt tggtgtctga tattcccaat
     6661 cttgcaaacc ataagatgcc accagctgct gctgaagaga ctcctccgta cagtaattat
     6721 aacacacttc cctgtaggaa atctcactgt ctctctgaag gtgccaccaa cccacaaatt
     6781 agccatagca acagcatgca aggcagaaga gctaaaacaa ctcaggatgt taatgcaggc
     6841 gagggcagtg agtttgcaga cagtgggatt gaaggggcca ctaccgacac ggacctcctg
     6901 tccaggcgat ctaatgccac caactccagc tactcaccca ccacaggccg ggcctttgtg
     6961 ggcagcgaca gcggcagcag ctccaccggg gatgcggctc gtcagggggt gtacgagaac
     7021 ttccggcggg agctggagat gagcaccacc aacagcgaga gcctggagga ggccggctcg
     7081 gcgcacagcg atgagcagag cagcggcacc ctgagctctc cgggccagtc ggacatcctg
     7141 ctgaccgccg cacagggcac ggtgcgcaag gccggcgccc tggccgtcaa gaacttcctg
     7201 gtgcacaaga agaacaagaa ggtggagtca gccacccgga ggaagtggaa gcactactgg
     7261 gtgtccctga aaggatgcac gctatttttc tacgagagcg acggcaggtc tgggatagac
     7321 cacaacagca tccccaaaca cgccgtctgg gtggagaaca gcattgtgca ggcggtgcct
     7381 gagcacccca agaaggactt tgtcttctgc ctcagcaatt ccctgggtga tgccttcctt
     7441 tttcagacca ctagccagac ggagcttgaa aactggatca ccgccatcca ctctgcctgc
     7501 gccactgcgg tcgcgaggca ccaccacaag gaagacacgc tccgactcct gaaatcagag
     7561 atcaaaaaac tggaacagaa gattgacatg gatgaaaaga tgaagaaaat gggtgaaatg
     7621 cagctgtctt cagtcactga ctcaaagaaa aagaaaacaa tattagatca gatctttgtc
     7681 tgggagcaaa atctcgagca gttccaaatg gacctgtttc gtttccgctg ttatttagcc
     7741 agccttcagg gtggggagct gccaaacccc aaaaggcttc tcgcttttgc aagtcgacca
     7801 acgaaagtgg ccatgggccg ccttggaatc ttttcggtat catcgtttca tgccctggtg
     7861 gcagcacgca ctggtgaaac tggagtgaga agacgtactc aggccatgtc cagatccgcg
     7921 agcaagcgaa ggagcaggtt ttcttctctg tggggtctgg atactacctc caaaaagaag
     7981 cagggacggc caagcatcaa tcaggtgttt ggagagggaa ccgaagctgt aaagaaatct
     8041 ttagagggaa tatttgatga cattgttcca gatggcaaga gggagaaaga agtggtctta
     8101 cctaacgttc accagcacaa ccctgactgc gacatttggg tccacgagta tttcactcca
     8161 tcctggttct gtctgcccaa taatcagcct gccctgacgg tcgtccggcc aggcgacact
     8221 gcacgggaca ccctggagct gatttgcaag acacatcaac tggatcattc tgctcattac
     8281 ctgcgcctga aatttctaat agaaaacaaa atgcagctct atgttccaca gcccgaggaa
     8341 gacatctatg agctgctgta caaagaaatt gaaatctgtc caaaagtcac tcagagcatc
     8401 cacattgaga agtcagatac agctgctgat acttacgggt tttcactttc ttctgtggaa
     8461 gaagatggta ttcgaaggct gtacgtgaat agtgtgaagg aaaccggttt agcttccaag
     8521 aaaggcctga aagcaggaga tgagattctt gagatcaata atcgtgctgc tgacgccctg
     8581 aactcttcta tgctcaaaga tttcctctca cagccctcgc tgggcctcct ggtgaggacc
     8641 taccccgagc tggaggaagg agtggagctg ctggaaagcc cgccccaccg agtggacggc
     8701 cctgccgacc ttggcgagag ccccctcgcc tttctcacca gcaacccagg gcacagcctt
     8761 tgcagcgagc agggcagcag tgctgagacc gctccagagg agaccgaggg gccagacttg
     8821 gaatcctcag atgagactga tcacagcagc aagagtacag aacaggtggc cgcattttgc
     8881 cgcagtttgc atgagatgaa cccctctgac cagagcccat ctcctcagga ctccacgggg
     8941 cctcagctgg cgaccatgag acaactctcg gatgcagata agctgcgcaa ggtgatctgc
     9001 gagctcctgg agacggagcg cacctacgtg aaggatttaa actgtcttat ggagagatac
     9061 ctaaagcctc ttcaaaaaga aacttttctc acccaggatg agcttgacgt gctttttgga
     9121 aatttaacgg aaatggtaga gtttcaagta gaattcctta aaactctaga agatggagtg
     9181 agactggtac ctgatttgga aaagcttgag aaggttgatc aatttaagaa agtgctgttc
     9241 tctctggggg gatcattcct gtattatgct gaccgcttca agctctacag tgccttctgc
     9301 gccagccaca caaaagttcc caaggtcctg gtgaaagcca agacagacac ggctttcaag
     9361 gcattcttgg atgcccagaa cccgaagcag cagcactcat ccacgctgga gtcgtacctc
     9421 atcaagccca tccagaggat cctcaagtac ccacttctgc tcagggagct gttcgccctg
     9481 accgatgcgg agagcgagga gcactaccac ctggacgtgg ccatcaagac catgaacaag
     9541 gttgccagtc acatcaatga gatgcagaaa atccatgaag agtttggggc tgtgtttgac
     9601 cagctgattg ctgaacagac tggtgagaaa aaagaggttg cagatctgag catgggagac
     9661 ctgcttttgc acactaccgt gatctggctg aacccgccgg cctcgctggg caagtggaaa
     9721 aaggaaccag agttggcagc attcgtcttc aaaactgctg tggtccttgt gtataaagat
     9781 ggttccaaac agaagaagaa acttgtagga tctcacaggc tttccattta tgaggactgg
     9841 gaccccttca gatttcgaca catgatcccc acggaagcgc tgcaggttcg agctttggcg
     9901 agtgcagatg cagaggcaaa tgccgtgtgt gaaattgtcc atgtaaaatc cgagtctgaa
     9961 gggaggccgg agagggtctt tcacttgtgc tgcagctccc cagagagccg aaaggatttc
    10021 ctaaaggctg tgcattcaat cctgcgtgat aagcacagaa gacagctcct caaaaccgag
    10081 agccttccct catcccagca atatgtccct tttggaggca aaagattgtg tgcactgaag
    10141 ggggccaggc cggccatgag cagggcagtg tctgccccaa gcaagtctct tgggaggagg
    10201 aggcggcggc tggctcgaaa caggtttacc attgattctg atgccgtctc cgcaagcagc
    10261 ccggagaaag agtcccagca gccccccggt ggcggggaca ctgaccgatg ggtagaggag
    10321 cagtttgatc ttgctcagta tgaggagcaa gatgacatca aggagacaga catcctcagt
    10381 gacgatgatg agttctgtga gtccgtgaag ggtgcctcag tggacagaga cctgcaggag
    10441 cggcttcagg ccacctccat cagtcagcgg gaaagaggcc ggaaaaccct ggatagtcac
    10501 gcgtcccgca tggcacagct caagaagcaa gctgccctgt cggggatcaa tggaggcctg
    10561 gagagcgcaa gcgaggaagt catttgggtt aggcgtgaag actttgcccc ctccaggaaa
    10621 ctgaacactg agatctgagc ggccgcagga ggcggtggag gcggtgcagg cgactacaaa
    10681 gaccatgacg gtgattataa agatcatgac attgattaca aggatgacga tgacaagtaa
    10741 taataaa
//
