LOCUS       sequence-172782-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant expression of PTBP1-RRM1 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A,
            Aebersold R, Allain FH
  TITLE     Structural modeling of protein-RNA complexes using crosslinking of 
            segmentally isotope-labeled RNA and MS/MS.
  JOURNAL   Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.
  PUBMED    28346450
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235."
COMMENT     Sequence Label: pTYB11-PTBP1-RRM1
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(400..948)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="modified 3' region of the intein from the yeast 
                     Vma1 subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 3' region
                     /note="C-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="GHGGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRE
                     TFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVT
                     KHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGI
                     TLSDDSDHQFLLGSQVVVQN"
ORIGIN
        1 cccttcctgc agtcacccgg gctcgagtca ggccaggttc cccgactgga ccgagttcac
       61 cgcctgcagg gccgcctggg cccgcgcctg gttgggagag ctgtcggtct tcagctcctt
      121 gtggttagag aactggatgt agatgggctg gccgcgcagc acaggggtca ccgaggtgta
      181 gtagttcacc atggtgttgg cagcctcctc cgtgttcatc tcgatgaagg cctggttttt
      241 ccccttcagc atcaggaggt tggtgacctt cccaaagggc agccccaggg agatgacttc
      301 cccctccgtg acgtcgatgg ggagcttccg gatgtggatc actctagagg ggacgcctgc
      361 acttcggctg tcacctttga acttcttgct gtcatttccg ttctgtacaa caacctggga
      421 tccaagcaaa aactgatgat cagaatcatc agataaagta atcccataat aatcgtcttc
      481 cttcaattct tgtaactcga aataaaatcc gcggcactca cgtgcaaaag cagcggcggg
      541 agcaggcctg aattttttag agccggcaca cttcgaaaga acgttaagca aaacatctcc
      601 accagacata taaatagcat aactaatttt atgtttggtg acattcatgt caaccttagc
      661 aggttctgcg ttaaccgaga ctactaagcc taaagaacga gcaagggaaa ccaaaccatc
      721 tctgacagaa gtatgaattg tctttattgt tgctttaata ccatgctcat cagtaacata
      781 gccatcagaa tcaattagac cagcaagaaa tgtttcacga gtaccgatat tgtccgtaga
      841 caagaaagaa ggaatatttt tgacaccgtc cttcaagaat cctaagccaa caatagcgtc
      901 ccataatgga ttctcagtat taagattatt gcgaatacca ccatggccac cttgaagctg
      961 ccacaaggca ggaacgttgg atggttccca tcctgccaag gaggtgtggg gctgca
//
