LOCUS       sequence-172785-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant expression of PTBP1-RRM34 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A,
            Aebersold R, Allain FH
  TITLE     Structural modeling of protein-RNA complexes using crosslinking of 
            segmentally isotope-labeled RNA and MS/MS.
  JOURNAL   Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.
  PUBMED    28346450
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235."
COMMENT     Sequence Label: pET28a-PTBP1-RRM34
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..66
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             706..723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc
       61 ggcagccata tgggtcggat cgccatcccg ggcctggcgg gggcaggaaa ttctgtattg
      121 ctggtcagca acctcaaccc agagagagtc acaccccaaa gcctctttat tcttttcggc
      181 gtctacggtg acgtgcagcg cgtgaagatc ctgttcaata agaaggagaa cgccctagtg
      241 cagatggcgg acggcaacca ggcccagctg gccatgagcc acctgaacgg gcacaagctg
      301 cacgggaagc cgatccgcat cacgctctcg aagcaccaga acgtgcagct gccccgcgag
      361 ggccaggagg accagggcct gaccaaggac tacggcaact cacccctgca ccgcttcaag
      421 aagccgggct ccaagaactt ccagaacata ttcccgccct cggccactct gcacctctcc
      481 aacatcccgc cctcagtctc cgaggaggat ctcaaggtcc tgttttccag caatgggggc
      541 gtcgtcaaag gattcaagtt cttccagaag gaccgcaaga tggcactgat ccagatgggc
      601 tccgtggagg aggcggtcca ggccctcatt gacctgcaca accacgacct cggggagaac
      661 caccacctgc gggtctcctt ctccaagtcc accatctagc tcgagcacca ccaccaccac
      721 cactgagatc cggctgctaa caaagccgaa agag
//
