LOCUS       sequence-172789-       11451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
            necessary for its activation by amino acids.
  JOURNAL   Cell. 2010 Apr 16. 141(2):290-303.
  PUBMED    20381137
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 16. 141(2):290-303."
COMMENT     Sequence Label: pLJM1 Flag Raptor Rheb15
FEATURES             Location/Qualifiers
     source          1..11451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2120..2140
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2429..2632
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2583..2603
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2673..2696
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6833..6950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6999..7508
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7427..7445
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7530..8129
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7530..7549)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8026..8046
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8257..8490
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8562..8683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8599..8618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8653..8672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8723..8858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8878..8897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8879..8897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8907..8924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8907..8923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8916..8938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9065..9520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9152..9171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9362..9383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9546..9650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9651..9719,9720..10511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9869..9888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10682..11270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11171..11190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11424..11441
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2041 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2101 agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     2161 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     2221 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2281 ttttaaaatg gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2341 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2401 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2461 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2521 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2581 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     2641 gaaccgtcag atccgctagc gctaccggta tggactacaa ggatgacgat gacaagggtg
     2701 gtggtggtgg tgcgtcgact atggagtccg aaatgctgca atcgcctctt ctgggcctgg
     2761 gggaggaaga tgaggctgat cttacagact ggaacctacc tttggctttt atgaaaaaga
     2821 ggcactgtga gaaaattgaa ggctccaaat ccttagctca gagctggagg atgaaggatc
     2881 ggatgaagac agtcagtgtt gccttagttt tgtgcctgaa tgttggtgtg gaccctcccg
     2941 atgtggtgaa gaccacgccc tgtgcacgct tggaatgctg gatcgatcct ctgtcgatgg
     3001 gtcctcagaa agctctggaa accatcggtg caaatttaca gaagcagtac gagaactggc
     3061 agccaagggc ccggtacaag cagagccttg acccaactgt ggatgaagtc aagaagctct
     3121 gcacgtcctt acgtcgcaac gccaaggagg agcgagtcct ctttcactac aatggccacg
     3181 gggtgccccg gcccacagtc aacggggagg tctgggtctt caacaagaac tacacgcagt
     3241 acatccctct gtccatatat gacctgcaga cgtggatggg cagcccgtcg atcttcgtct
     3301 acgactgctc caatgctggc ttgatcgtca agtccttcaa gcagttcgca ctacagcggg
     3361 agcaggagct ggaggtagct gcaatcaacc caaatcaccc tcttgctcag atgcctttgc
     3421 ctccgtcgat gaaaaactgc atccagctgg cagcctgcga ggccaccgag ctgctgccca
     3481 tgatccccga cctcccggct gacctattca cctcctgcct caccaccccc atcaagatcg
     3541 ccctgcgctg gttttgcatg cagaaatgtg tcagtctggt gcctggcgtc acactggatt
     3601 tgatagaaaa gatccctggc cgcctgaacg acaggaggac gcccctgggt gaactgaact
     3661 ggatcttcac agccatcaca gacaccatcg cgtggaacgt gctcccccgg gatctcttcc
     3721 aaaagctctt cagacaggac ttgctggtgg ctagtctgtt tcgaaatttt ttattggcgg
     3781 aaaggattat gaggtcgtat aactgcactc ccgtcagcag cccgcgtctg ccgcccacgt
     3841 acatgcacgc catgtggcaa gcctgggacc tggctgttga catctgtctg tctcagctgc
     3901 cgacgatcat cgaggaaggc actgcgtttc ggcacagccc gttcttcgcc gagcagctga
     3961 ccgcattcca ggtgtggctc accatgggcg tggagaaccg aaacccaccc gaacagctgc
     4021 ccatcgtcct gcaggtgctg ttaagccaag tgcaccggct gagagcattg gacttgcttg
     4081 gaagattttt ggacctgggt ccctgggcag tgagcctggc cttgtctgtc ggcatcttcc
     4141 cctacgtgct gaagctgctc cagagctcgg cccgagagct gcggccactt ctcgttttca
     4201 tctgggccaa gatcctcgca gtggacagct cgtgccaggc ggacctcgtg aaggacaacg
     4261 gccacaagta cttcctgtcg gtcctggcgg acccctacat gccagctgaa caccggacca
     4321 tgacggcttt cattctcgcc gtgatcgtca acagctatca cacggggcag gaagcctgcc
     4381 ttcagggaaa cctcattgcc atctgcctgg agcagctcaa cgacccgcac cccttgctgc
     4441 gccagtgggt ggccatctgc ctcggcagga tctggcagaa cttcgactcg gcgaggtggt
     4501 gcggcgtgag ggacagcgct catgagaagc tctacagcct cctctccgac cccattcccg
     4561 aggtccgctg cgcagcggtc ttcgcccttg gcacgttcgt gggcaactct gcagagagga
     4621 cggaccactc caccaccatc gaccacaacg tggccatgat gctggcccag ctggtcagcg
     4681 acgggagccc catggtccgg aaggagctgg tggtggctct gagtcatctt gtggttcagt
     4741 atgaaagcaa tttctgcacc gtggccctgc agttcataga agaggaaaag aactacgcct
     4801 tgccttctcc agcaaccaca gagggaggga gtttgacccc agtgcgagac agcccgtgca
     4861 cccccagact tcgttctgtg agctcctatg gaaacatccg tgctgtcgcc acagccagga
     4921 gcctcaacaa atctttgcag aacctgagtt tgacagagga atctggtggc gcggtggcgt
     4981 tctcccccgg aaacctcagc accagcagca gcgccagcag caccctgggc agccccgaga
     5041 atgaggagca tatcctgtcc ttcgagacca tcgacaagat gcgccgcgcc agctcctact
     5101 cctccctcaa ctccctcatc ggagtttcct ttaacagtgt ttacactcag atttggagag
     5161 tcctgctgca cctggctgct gacccctatc cagaggtctc ggacgtggcc atgaaagtac
     5221 tcaacagcat cgcctacaag gccaccgtga acgcccggcc gcagcgcgtc ctggacacct
     5281 cctccctcac gcagtcggcc cccgccagcc ccaccaacaa gggcgtgcac atccaccagg
     5341 cggggggctc ccctccggcg tccagcacca gcagctccag cctgaccaac gatgtggcca
     5401 agcagccggt cagccgagac ttgccttctg gccggccggg caccacaggc cccgctgggg
     5461 cgcagtacac ccctcactcc caccagttcc cccggacacg gaagatgttc gacaagggcc
     5521 cagagcagac tgcggacgac gcggacgatg ctgctggaca caaaagtttc atctccgcca
     5581 cggtgcagac ggggttctgc gactggagcg cccgctattt tgcccagccc gtcatgaaga
     5641 tcccagaaga gcacgacctg gagagtcaga tccgcaagga gcgggagtgg cggttcctgc
     5701 gaaacagccg tgtcaggagg caggcccagc aagtcattca gaagggcatt acgagattgg
     5761 acgaccaaat atttctgaac aggaaccccg gcgtcccctc tgtggtgaaa ttccacccct
     5821 tcacgccgtg catcgctgta gccgacaagg acagcatctg cttttgggac tgggagaaag
     5881 gggagaagct ggattatttc cacaatggga accctcggta cacgagggtc actgccatgg
     5941 agtatctgaa tggccaggac tgctcgcttc tgctgacggc cacagacgat ggtgccatca
     6001 gggtctggaa gaattttgct gatttggaaa agaacccaga gatggtgacc gcgtggcagg
     6061 ggctctcgga catgctgcca acgacgcgag gagctgggat ggtggtggac tgggagcagg
     6121 agaccggcct cctcatgagc tcaggagacg tgcggatcgt ccggatctgg gacacagacc
     6181 gtgagatgaa ggtgcaggac atccctacgg gcgcagacag ctgtgtgacg agtctgtcct
     6241 gtgattccca ccgctcactc atcgtggctg gcctcggtga cggctccatc cgcgtctacg
     6301 acagaaggat ggcactcagc gaatgccgcg tcatgacgta ccgggagcac acagcctggg
     6361 tggtgaaggc ctccctgcag aagcgtcccg acggccacat cgtgagtgtg agcgtcaatg
     6421 gagatgtgcg catctttgat ccccggatgc ctgagtcggt aaatgtgctt cagatcgtga
     6481 aggggctgac ggccctggac atccaccccc aggcggacct gatcgcatgt ggctccgtca
     6541 atcagttcac cgccatctac aacagcagcg gagagctcat caacaacatc aagtactacg
     6601 acggcttcat gggccagcgg gtcggcgcca tcagctgcct ggccttccac ccgcactggc
     6661 ctcacctggc cgtgggaagc aacgactact acatctccgt gtactcggtg gagaagcgtg
     6721 tcagagatat cggtggaggc atggacgggg cagcttcaca agggaagtct tcgtgctcgg
     6781 tgatgtgatt cgaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     6841 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     6901 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     6961 acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc
     7021 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cggaaacgca
     7081 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     7141 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     7201 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     7261 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     7321 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     7381 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     7441 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     7501 ctccccaggg ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg
     7561 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7621 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7681 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7741 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     7801 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     7861 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     7921 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7981 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8041 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8101 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     8161 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg
     8221 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8281 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8341 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8401 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8461 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8521 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8701 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8821 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     8881 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8941 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9001 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9061 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9481 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9541 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9601 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9661 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9721 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9781 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9841 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9901 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9961 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10021 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10081 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10141 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10201 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10261 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10321 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10381 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10441 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10501 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10561 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11341 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa g
//
