LOCUS       sequence-172792-        6708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing NDUFAF5-HA-IRES-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs-NDUFAF5-HA
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    3..555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(147..164)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     374..393
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             567..962
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1208..1677
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1807..1824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1978..2566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2058..2077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2730..3521,3522..3590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3353..3372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3591..3695)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3726..4242
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    4306..4663
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4722..5138
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5068..5090
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5106..5122
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5148..5522
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5460..5476
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             6622..6648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6671..6694
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
       61 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
      121 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
      181 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
      241 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
      301 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
      361 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
      421 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
      481 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      541 aacacgatga taatatggcc acaaccatgg ttcctttgtc tcaagaagaa tccaccctca
      601 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
      661 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
      721 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
      781 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
      841 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
      901 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
      961 aagtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     1021 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     1081 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     1141 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1201 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     1261 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     1321 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     1381 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     1441 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     1501 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     1561 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     1621 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat
     1681 gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata
     1741 gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2641 gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa
     2701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3721 gcataatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     3781 aggcatggaa aaatacataa ctgagaatag aaaagttcag atcaaggtca ggaacagatg
     3841 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     3901 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     3961 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     4021 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     4081 cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     4141 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     4201 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     4261 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
     4321 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
     4381 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
     4441 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     4501 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     4561 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     4621 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     4681 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     4741 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     4801 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     4861 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     4921 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     4981 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     5041 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     5101 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     5161 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca agagttacta
     5221 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     5281 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     5341 gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga
     5401 aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag
     5461 cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac
     5521 tgccggatct agctagttaa ttaaggatcc gccaccatgc tgcggccggc agggctctgg
     5581 cgcttatgtc ggcgaccttg ggcggcgagg gtcccagcgg agaatcttgg ccgtagggaa
     5641 gtcacctctg gtgtctctcc ccgcggtagc acctcgccca gaaccctgaa tattttcgac
     5701 cgggatttga aaaggaaaca gaagaactgg gcagcccggc agcccgagcc gaccaaattt
     5761 gactacctga aggaggaggt tggaagtcgg atcgcagacc gtgtatatga catacccaga
     5821 aatttccccc ttgctttgga tcttggttgt ggaagaggtt acattgcaca atatttgaat
     5881 aaggaaacta ttggaaagtt tttccaagct gacattgcag aaaatgcttt gaaaaattcc
     5941 tcagaaacag aaatacctac tgtcagcgtt ttagctgatg aagaattcct tcccttcaaa
     6001 gaaaatacat ttgacctggt ggttagcagt ttaagtttgc attgggtgaa tgaccttcct
     6061 agagcacttg agcagattca ttatatttta aaaccagatg gagtgtttat cggtgcaatg
     6121 tttggaggcg acacactcta tgaacttcgg tgttccttac agttagcgga aacggaaagg
     6181 gaaggaggat tttctccaca catttctcct ttcactgctg tcaatgacct gggacatctg
     6241 cttgggagag ctggctttaa tactctgact gtggacactg atgaaattca agttaactat
     6301 cctgggatgt ttgaattgat ggaagattta caaggtatgg gtgagagtaa ctgtgcttgg
     6361 aatagaaaag ccctgctgca tcgagacaca atgctggcag ctgcggcagt gtacagagaa
     6421 atgtacagaa atgaagatgg ttcagtacct gctacatacc agatctatta catgatagga
     6481 tggaaatatc atgagtcaca ggcaagacca gctgaaagag gttccgcaac tgtgtcattt
     6541 ggagagctag gaaaaataaa caaccttatg ccaccaggga aaaaatcaca agcggccgca
     6601 ggaggcggtg gaggcggtgg atacccctat gatgtgcctg actacgcatg attataaaga
     6661 tcatgacatt gattacaagg atgacgatga caagtaataa taaattcc
//
