LOCUS       sequence-172808-        4430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Rotavirus SA11 VP6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, 
            Matsuura Y, Taniguchi K, Kobayashi T
  TITLE     Entirely plasmid-based reverse genetics system for rotaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114.
  PUBMED    28137864
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 
            10.1073/pnas.1618424114."
COMMENT     Sequence Label: pT7-VP6SA11
FEATURES             Location/Qualifiers
     source          1..4430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1533..1551)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1547..1594
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1662..1680)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1663..1680)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1970..1989)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2199..2218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2318..2340
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2378..2396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2464..2568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2569..2637,2638..3429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2787..2806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3600..4188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4089..4108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4342..4359
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggttaatacg actcactata ggcttttaaa cgaagtcttc aacatggatg
       61 tcctatactc tttgtcaaag actcttaaag acgctagaga caaaattgtc gaaggcacat
      121 tgtattctaa cgtgagtgat ctaattcaac aatttaatca aatgataatt actatgaatg
      181 gaaatgaatt tcaaactgga ggaatcggta atttgccaat tagaaactgg aattttaatt
      241 tcgggttact tggaacaact ttgctgaact tagacgctaa ttatgttgaa acggcaagaa
      301 atacaattga ttatttcgtg gattttgtag acaatgtatg catggatgag atggttagag
      361 aatcacaaag gaacggaatt gcacctcaat cagactcgct aagaaagctg tcagccatta
      421 aattcaaaag aataaatttt gataattcgt cggaatacat agaaaactgg aatttgcaaa
      481 atagaagaca gaggacaggt ttcacttttc ataaaccaaa catttttcct tattcagcat
      541 catttacact aaatagatca caacccgctc atgataattt gatgggcaca atgtggttaa
      601 acgcaggatc ggaaattcaa gtcgctggat ttgactactc atgtgctatt aacgcaccag
      661 ccaatataca acaatttgag catattgtgc cactccgaag agtgttaact acagctacga
      721 taactcttct accagacgcg gaaaggttta gttttccaag agtgatcaat tcagctgacg
      781 gggcaactac atggtttttc aacccagtga ttctcaggcc gaataacgtt gaagtggagt
      841 ttctattgaa tggacagata ataaacactt atcaagcaag atttggaact atcgtagcta
      901 gaaattttga tactattaga ctatcattcc agttaatgag accaccaaac atgacaccag
      961 cagtagcagt actattcccg aatgcacagc cattcgaaca tcatgcaaca gtgggattga
     1021 cacttagaat tgagtctgca gtttgtgagt ctgtactcgc cgatgcaagt gaaactctat
     1081 tagcaaatgt aacatccgtt aggcaagagt acgcaatacc agttggacca gtctttccac
     1141 caggtatgaa ctggactgat ttaatcacca attattcacc gtctagggag gacaatttgc
     1201 aacgcgtatt tacagtggct tccattagaa gcatgctcat taaatgagga ccaagctaac
     1261 aacttggtat ccaactttgg tgagtatgta gctatatcaa gctgtttgaa ctctgtaagt
     1321 aaggatgcgt atacgcattc gctacacaga gtaatcactc agatggtata gtgagaggat
     1381 gtgaccgggt cggcatggca tctccacctc ctcgcggtcc gacctgggca tccgaaggag
     1441 gacgtcgtcc actcggatgg ctaagggaga gctcaaaaaa aaggatccgg ctgctaacaa
     1501 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     1561 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
     1621 gagatcctct agagtcgacc tgcaggcatg caagcttgta ttctatagtg tcacctaaat
     1681 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     1741 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     1801 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     1861 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     1921 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     1981 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     2041 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcaccat
     2101 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ttaatgcagg
     2161 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
     2221 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2281 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     2341 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     2401 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     2461 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2521 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2581 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2641 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2701 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2761 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2821 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2881 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2941 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3001 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3061 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3121 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3181 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3241 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3301 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3361 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3421 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3481 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3541 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3601 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3661 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3721 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3781 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3841 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3901 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3961 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4021 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4081 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4201 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4261 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4321 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4381 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagggggat
//
