LOCUS       sequence-172815-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo GFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: pCW57-GFP-P2A-MCS (Neo)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..764
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 trrcwwwtag cagagctcgt ttagtgaccg tcagatcgcc tggagaattg gctagcgaat
       61 tcgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca
      121 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccgagc
      181 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc
      241 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg
      301 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt
      361 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga
      421 tcggcgactt caaggtgatg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca
      481 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgatctgg
      541 atggcagctt cacccgcacc ttcagcctgc gcgacggcgg ctactacagc tccgtggtgg
      601 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca
      661 tgttcgcctt ccgccgcgtg gaggaggatc acagcaacac cgagctgggc atcgtggagt
      721 accagcacgc cttcaagacc ccggatgcag atgccggtga agaaaccggt tccggagcca
      781 cgaacttctc tctgttaaag cagcagagac gtggagaaac ccggtcctag ctgcagacgc
      841 gtgtamgatc ggggggttgg gtgcgctttc agcagcctgg tttgcgcagg acgcgctgcy
      901 ytgggcgkgg tt
//
