LOCUS       sequence-172816-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo GFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: pCW57-GFP-P2A-MCS (Neo)
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             369..425
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 crrccggcgc gtgacggcga cttcaggtga tgggcaccgg cttccccgag gacagcgtga
       61 tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg
      121 ataacgatct ggatggcagc ttcacccgca ccttcagcct gcgcgacggc ggctactaca
      181 gctccgtggt ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga
      241 acgggggccc catgttcgcc ttccgccgcg tggaggagga tcacagcaac accgagctgg
      301 gcatcgtgga gtaccagcac gccttcaaga ccccggatgc agatgccggt gaagaaaccg
      361 gttccggagc cacgaacttc tctctgttaa agcaagcagg agacgtggaa gaaaaccccg
      421 gtcctaggct gcagacgcgt tgtacaggat ccggggttgg ggttgcgcct tttccaaggc
      481 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
      541 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
      601 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
      661 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
      721 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
      781 agcggctgct cagcaggcgc gccgagagca gcggccggaa gggcgtgcgg agcggtgtgg
      841 gggssgsssw wwwgtgkggg cccykkgttc cykscccgss sggkkgttcc ssmwttytgs
      901 magcctccsg ragsscmskt cggcarwycg gcttcatg
//
