LOCUS       sequence-172817-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo RFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: pCW57-RFP-P2A-MCS (Neo)
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             69..761
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 tgmtwwaagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg gctagcgaat
       61 tcgccaccat gagcgagctg atcaaggaga acatgcacat gaagctgtac atggagggca
      121 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
      181 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
      241 tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg
      301 acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag
      361 acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca
      421 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
      481 gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca
      541 gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat
      601 acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca
      661 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
      721 tggccaagta ctgcgacctc cctagcaaac tggggcacag aaccggttcc ggagccacga
      781 acttctctct gttaaagcaa gcaggagacg kgtagaaacc ccggtcctag ctgcagacgc
      841 gttgtacaga tccggggttg gggtgcgctt tcagcagcct gggtttgcsc agggacgcgg
      901 ctgcyyctg
//
