LOCUS       sequence-172818-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one gene in combination with turbo RFP using the P2A self-cleaving 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Barger CJ, Chee L, Albahrani M, Munoz-Trujillo C, Boghean L, Branick
            C, Odunsi K, Drapkin R, Zou L, Karpf AR
  TITLE     Co-regulation and function of FOXM1/RHNO1 bidirectional genes in 
            cancer.
  JOURNAL   Elife. 2021 Apr 23;10. pii: 55070. doi: 10.7554/eLife.55070.
  PUBMED    33890574
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-04-23"; volume: "10. pii: 55070. doi"; pages: " 
            10.7554/eLife.55070"
COMMENT     Sequence Label: pCW57-RFP-P2A-MCS (Neo)
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             393..449
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 csstmcaggg mccagcttca gacggctgca tcatctacaa cgtcaagatc aacggggtga
       61 acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg
      121 agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg
      181 tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta
      241 agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg
      301 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc
      361 ctagcaaact ggggcacaga accggttccg gagccacgaa cttctctctg ttaaagcaag
      421 caggagacgt ggaagaaaac cccggtccta ggctgcagac gcgttgtaca ggatccgggg
      481 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
      541 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      601 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      661 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      721 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      781 cgaccgcgat gggctgtggc catagcggct gctcagcagg cgcgcgagag cacggcggca
      841 cgcgtgcgag cgggtktggg gssgysawwt gtgtgacckk tyycksgccc gscgsgkkkt
      901 tccgcwwtyc kgcagcyctc gggaasssmy mgykcggcak ycggkyccyc tcskktggc
//
