LOCUS       sequence-172819-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   -
  TITLE     Karpf Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Neo)
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             729..838
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDLTLTC"
ORIGIN
        1 gttcgcattc tgcagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
       61 accgacctct ctccccagca attcacccgc gcgcggccac catgtctaga ctggacaaga
      121 gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga
      181 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
      241 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
      301 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
      361 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
      421 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
      481 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
      541 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
      601 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
      661 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
      721 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
      781 actttgacct tgatatgctg cctgctgacg ctcttgacga tttgaccttg acatgctccc
      841 cggggtcgat gaagcgagaa ggtagaggtc tctcctcmtt gtgtgatgtg agaaaacctg
      901 tcaatgtcta gatgatgaac agatgattgc mcgcagtctc ggcsckkggg gtgggaragg
      961 ctattcggct wtga
//
