LOCUS       sequence-172820-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   -
  TITLE     Karpf Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Neo)
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     705..724
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttycymttgt ggkgwgttga gaaccctggt ccatgtctag aatgattgaa caagatggat
       61 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
      241 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      301 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      361 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      421 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      481 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      541 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
      601 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
      661 tcgactgtgg ccggctgggt gtggcggacc gctatcagac atagcgttgg ctacccgtga
      721 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
      781 cgctcccgat tcgcagcgca tcgcttctat cgccttcttg acgagttctc tgatgcattg
      841 agatcgatcc agatatcgta tctactatgt gctctttacg ctatgtgawa cgctgcttaa
      901 tgccttgtat catgctattg gc
//
