LOCUS       sequence-172822-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   -
  TITLE     Karpf Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Neo)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     321..340
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(492..512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 acwtgactga tgcatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
       61 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
      121 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
      181 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
      241 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
      301 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
      361 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
      421 cttctatcgc cttcttgacg agttcttctg aatgcattga ggatcgatcc aagatatcgt
      481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
      601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
      661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
      721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
      781 tggacagggg ctcggctgtt ggcactgaca attccgtggt gttgtcggga agctgacgtc
      841 ctttccatgg ctgctcgcct gtgtgccacc tggattctgc gcgggacgtc cttctgctac
      901 gtcccttc
//
