LOCUS       sequence-172844-       10058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  P. falciparum integration plasmid for N-terminal gene fusions and 
            placing loxP sites using SLI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, 
            Jonscher E, Bergmann B, Spielmann T
  TITLE     A genetic system to study Plasmodium falciparum protein function.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
  PUBMED    28288121
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223."
COMMENT     Sequence Label: pSLI-N-GFP-2xFKBP-loxP(K13)
FEATURES             Location/Qualifiers
     source          1..10058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1321..1340)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1322..1340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1347..1364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1347..1363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1356..1378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1572..1591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1691..1713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1751..1769)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1837..1941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1942..2010,2011..2802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2160..2179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2973..3561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3462..3481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3715..3732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3849..3870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3885..3890,3891..3908,3909..3915)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3923..3939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3928..3950
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3947..3963
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3947..3963
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3981..3999
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3981..3998
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4575..4604
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4608..4637
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(4638..4671)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4677..4730
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5676..5729
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5736..6449
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5736..6449
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5766..5794)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6389..6409
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6522..6842
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6849..7169
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    complement(9411..9444)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaataaataa tataatttat aattatatat tcttgtcaca ataaaaatat atatatatat
       61 atatatattt ataatatgta tattttaaac tagaaaagga ataactaata ttttatttat
      121 tatcattcaa gatttatatt ttataataat aaatacctaa tagaaatata tcaggatcca
      181 tgcatggttc gctaaactgc atcgttgctg tgtcccagaa catgggcatc ggcaagaacg
      241 gggactaccc ctggccaccg ctcaggaacg aatttagata tttccagaga atgaccacaa
      301 cctcttcagt agaaggtaaa cagaatctgg tgattatggg taagaagacc tggttctcca
      361 ttcctgagaa gaatcgacct ttaaagggta gaattaattt agttctcagc agagaactca
      421 aggaacctcc acaaggagct cattttcttt ccagaagtct agatgatgcc ttaaaactta
      481 ctgaacaacc agaattagca aataaagtag acatggtctg gatagttggt ggcagttctg
      541 tttataagga agccatgaat cacccaggcc atcttaaact atttgtgaca aggatcatgc
      601 aagactttga aagtgacacg ttttttccag aaattgattt ggagaaatat aaacttctgc
      661 cagaataccc aggtgttctc tctgatgtcc aggaggagaa aggcattaag tacaaatttg
      721 aagtatatga gaagaatgat taagcttatt taataataga ttaaaaatat tataaaaata
      781 aaaacataaa cacagaaatt acaaaaaaaa tacatatgaa tttttttttg taatcttcct
      841 tataaatata gaataatgaa tcatataaaa catatcatta ttcatttatt tacatttaaa
      901 attattgttt cagtatcttt aatttattat gtatatataa aaataactta caattttatt
      961 aataagcaat atatgtttat taattcatgt tttgtaattt atgggatagc gatttttttt
     1021 actgtctgta tttttctttt ttaattatgt tttaattgta ttttattttt attattgttc
     1081 tttttatagt attattttaa aacaaaatgt attttctaag aacttataat aataataaat
     1141 ataaatttta ataaaaatta tatttatctt ttacaatatg aacataaagt acaacattaa
     1201 tatatagctt ttaatatttt tattcctaat catgtaaatc ttaaattttt ctttttaaac
     1261 atatgttaaa tatttatttc tcattatata taagaacata tttattaaat ctagaattcg
     1321 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     1381 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1441 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1501 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1621 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1681 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1741 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3601 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3661 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3721 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3781 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3841 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3901 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     3961 gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct gcggccgcta
     4021 aagatgcagc aaatcttata aatgatgatt ctggattaaa caatttaaaa aaattttcaa
     4081 ctgtaaataa tgtaaatgat acttatgaaa agaaaattat tgaaacggaa ttaagtgatg
     4141 ctagtgattt tgaaaatatg gtaggtgatt taagaattac atttattaat tggttaaaaa
     4201 agacacaaat gaattttatt cgagaaaaag ataaattatt taaagataag aaagaactag
     4261 aaatggaaag agtacgattg tacaaagaat tagaaaaccg taaaaatatt gaagaacaga
     4321 aattacatga tgaaagaaag aaattagata ttgatatatc taatggttat aaacaaataa
     4381 aaaaagaaaa agaagaacat aggaaacgat ttgatgaaga aagattaaga tttttacaag
     4441 aaatcgataa aattaaatta gtattatatt tagaaaaaga aaaatattat caagaatata
     4501 aaaattttga gaatgataaa aaaaaaattg ttgatgcaaa tattgctact gaaactatga
     4561 ttgatggttt aaacgagcag aagttaatat cagaagagga tttgggtgaa caaaaactca
     4621 taagcgaaga agatttaata acttcgtata gcatacatta tacgaagtta tccggagaag
     4681 gaagaggaag tttattaaca tgtggagatg tagaagaaaa tccaggacca atgacagcca
     4741 gtttaactac caagttcttg aacaatacct atgaaaaccc atttatgaat gcatccggtg
     4801 ttcattgcat gactacacaa gaattagatg aattagcaaa ctctaaagct ggcgcattca
     4861 ttacaaagag tgctacaacc ttagaaagag aaggtaaccc tgaaccacgt tacatttctg
     4921 tccctctagg cagtatcaac tccatgggtt taccaaacga aggtatcgac tactatttgt
     4981 cctatgtatt aaaccgtcaa aagaattatc ctgatgcacc tgctattttc ttctcagttg
     5041 ctggtatgag cattgatgaa aatttaaatt tgttgaggaa aatccaagat agcgaattca
     5101 acggtattac cgagttaaac ttgtcttgtc ctaatgtgcc tgggaaacca caagttgctt
     5161 atgactttga cttgacaaag gaaaccttgg aaaaggtttt tgcctttttc aaaaaacctc
     5221 ttggtgtcaa gttgcctcct tattttgatt ttgcccattt tgatatcatg gcaaaaatat
     5281 tgaacgagtt cccattagct tatgtcaact ctatcaatag tataggaaat ggtcttttca
     5341 ttgatgtgga gaaggagagt gtagtagtga agccaaagaa tggtttcggg ggtattggag
     5401 gtgaatatgt taagccaacc gcgctcgcca atgttcgtgc attttacact cgtttgagac
     5461 ctgaaatcaa agttatcggt acaggtggaa ttaagtccgg taaggatgca tttgaacatc
     5521 ttctatgtgg tgcctctatg ctacagattg gtacagaatt acaaaaagag ggcgtcaaga
     5581 tttttgaacg tatcgaaaaa gaattaaaag acataatgga agctaagggt tatacatcca
     5641 tagatcagtt ccgtgggaag ttgaacagca ttggtgaagg tagaggttct ttgttgactt
     5701 gtggtgatgt tgaagaaaat ccaggtccag ctagcatgag taaaggagaa gaacttttca
     5761 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     5821 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     5881 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcgcg tatggtcttc
     5941 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     6001 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     6061 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     6121 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     6181 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     6241 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     6301 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     6361 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     6421 ttacacatgg catggatgaa ctatacaaac ctagctcagg attgagatca agatctgctg
     6481 ctgctggtgc tggtggtgct gctagagctg ctctgcagag aggagtacaa gttgaaacaa
     6541 tatcaccagg agatggtcgt acatttccaa aaagaggtca aacttgtgtt gtacattata
     6601 ctggaatgct tgaagatgga aagaaatttg attcatctcg tgatagaaat aaaccattta
     6661 aatttatgct aggtaaacaa gaagtaatac gaggttggga agaaggagtt gctcaaatga
     6721 gtgtaggtca aagagcaaaa cttactatat ctccagatta tgcttatggt gcaactggac
     6781 atccaggtat aattccacct catgcaactc ttgtatttga tgtggagctt ctaaaactag
     6841 aaactagagg tgttcaggtt gaaacaattt cacctggaga tggcagaacc tttcctaaaa
     6901 gaggacagac ttgcgtagtt cattatacag gcatgctaga ggatggtaag aaatttgatt
     6961 ctagtcgaga tagaaataag ccattcaagt ttatgctagg taaacaggaa gtaataagag
     7021 gttgggaaga gggtgtagca cagatgtcag ttggacaaag agcaaagtta acaatatcac
     7081 cagattatgc atacggtgca acaggccatc ctggcatcat ccctccacat gcaactttag
     7141 tattcgacgt tgaattgtta aagttagaga caacgcgtgc tagaggtgct gctgctggtg
     7201 ctggaggtgc aggtagacct aggatggagg gtgagaaggt taagactaaa gctaacagta
     7261 taagtaactt cagtatgact tacgacagag agagtggagg gaatagtaac tcagacgaca
     7321 agagtggttc aagttcagaa aacgactcaa acagttttat gaacttaaca tctgacaaga
     7381 acgaaaagac agagaacaac tcatttttac ttaacaactc atcttacggt aacgtaaagg
     7441 actctttatt ggagtctata gacatgtcag tgttggacag taatttcgac tcaaagaagg
     7501 acttcttgcc ttcaaacttg tctaggactt tcaacaacat gagtaaggac aacattggta
     7561 acaagtactt gaacaagtta ttgaacaaga agaaggacac aataactaac gagaacaaca
     7621 acataaacca caacaacaac aacaacaact taactgctaa caacattaca aacaacttaa
     7681 taaacaacaa catgaactca ccttctataa tgaacacaaa taagaaggaa aacttcttgg
     7741 acgctgctaa cttgattaac gacgactcag gtttgaataa cttgaagaag ttctctacag
     7801 ttaacaacgt taacgacaca tacgagaaaa agataataga gaccgagttg tcagacgcat
     7861 cagacttcga gaacatggtt ggagacttga ggataacttt cataaactgg ttgaagaaaa
     7921 ctcagatgaa cttcataaga gagaaggaca agttgttcaa ggacaaaaag gagcttgaga
     7981 tggagagggt tcgtttatat aaggagttgg agaatcgtaa gaacatagag gagcaaaagt
     8041 tgcacgacga gaggaaaaag ttggacatag acatttcaaa cggatacaag cagattaaga
     8101 aggagaagga ggagcacaga aagcgtttcg acgaggagag gttgaggttc ttgcaggaga
     8161 tagacaagat aaagttggtt ttgtacttgg agaaggagaa gtactaccag gagtacaaga
     8221 acttcgaaaa cgacaagaag aagatagtag acgctaacat agcaacagag acaatgatag
     8281 acataaacgt aggaggtgca atattcgaga cttcaaggca cactttgact cagcagaagg
     8341 actctttcat tgaaaagttg ttgtcaggta ggcaccacgt tacaagggac aagcagggta
     8401 ggattttttt ggacagagac tcagaattgt tcaggataat tttaaatttc ttgaggaacc
     8461 ccttgacaat tcctattcct aaggacttgt ctgagtcaga ggctttatta aaggaggctg
     8521 agttctacgg aataaagttc ttgccttttc ctttggtttt ctgcattgga ggtttcgacg
     8581 gagttgagta cttgaacagt atggagttgt tggacatatc acagcagtgt tggcgaatgt
     8641 gcactccaat gtcaactaag aaggcatact tcggttctgc agttttaaac aactttttgt
     8701 atgtattcgg aggaaacaat tacgactaca aagcattgtt cgagacagag gtatacgaca
     8761 ggttgaggga cgtttggtac gtaagttcaa acttgaacat tccaaggagg aacaactgcg
     8821 gagtaacctc taacggaagg atatactgca taggaggtta cgacggatca tcaataattc
     8881 ccaacgttga ggcttacgac cacagaatga aggcttgggt tgaagtagct ccattaaaca
     8941 ctccaaggtc tagtgcaatg tgcgtagcat tcgacaacaa gatatacgtg ataggaggta
     9001 caaacggaga aaggttgaac tcaatagagg tttacgagga gaagatgaac aagtgggagc
     9061 agttccctta cgcattgttg gaggcaaggt caagtggtgc tgcattcaac tatttaaacc
     9121 agatttacgt agtaggtgga atagacaacg agcacaatat tttggactct gtagagcagt
     9181 accagccttt caacaagagg tggcagttcc ttaacggagt tcctgaaaag aagatgaact
     9241 tcggtgcagc tactttaagt gactcataca ttataactgg tggtgagaac ggggaggtac
     9301 ttaactcttg ccactttttc tcacctgaca ctaacgagtg gcaattagga ccttcattgt
     9361 tggtaccaag gttcggacat tcagtattga ttgctaacat ttgaaggcct ataacttcgt
     9421 atagcataca ttatacgaag ttattatgac tcgaggatat ggcagcttaa tgttcgtttt
     9481 tcttatttat atatttatac caattgattg tatttataac tgtaaaaatg tgtatgttgt
     9541 gtgcatattt ttttttgtgc atgcacatgc atgtaaatag ctaaaattat gaacatttta
     9601 ttttttgttc agaaaaaaaa aactttacac acataaaatg gctagtatga atagccatat
     9661 tttatataaa ttaaatccta tgaatttatg accatattaa aaatttagat atttatggaa
     9721 cataatatgt ttgaaacaat aagacaaaat tattattatt attattattt ttactgttat
     9781 aattatgttg tctcttcaat gattcataaa tagttggact tgatttttaa aatgtttata
     9841 atatgattag catagttaaa taaaaaaagt tgaaaaatta aaaaaaaaca tataaacaca
     9901 aatgatgttt tttccttcaa tttcgatatc aatttataga aacaaaatat atacttgtat
     9961 aattttattt ttttatataa atcattacat atataattat acaatatttt ttctaagaga
    10021 taattatata ttaatatata taaaaaaagg tgtttttt
//
