LOCUS       sequence-17288-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Hartley RW
  TITLE     Barnase and barstar. Expression of its cloned inhibitor permits 
            expression of a cloned ribonuclease.
  JOURNAL   J Mol Biol 1988 Aug 20;202(4):913-5.
  PUBMED    3050134
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol"; date: "1988-08-20"; volume: "202(4)"; pages: "913-5"
COMMENT     Sequence Label: pMT413
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16..38
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     sig_peptide     72..134
                     /codon_start=1
                     /product="signal sequence of E. coli alkaline phosphatase 
                     (Inouye et al., 1982)"
                     /label=phoA signal sequence
                     /translation="MKQSTIALALLPLLFTPVTKA"
     CDS             135..467
                     /codon_start=1
                     /product="ribonuclease from Bacillus amyloliquefaciens"
                     /label=barnase
                     /note="The barnase gene is lethal in standard bacterial 
                     transformation strains."
                     /translation="AQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADV
                     APGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQ
                     TFTKIR"
ORIGIN
        1 tggtgtggaa ttgtgagcgg ataacaattt cacacaggaa acaggatccg cggatccgtg
       61 gagaaaataa agtgaaacaa agcactattg cactggcact cttaccgtta ctgtttaccc
      121 ctgtgacaaa agccgcacag gttatcaaca cgtttgacgg ggttgcggat tatcttcaga
      181 catatcataa gctacctgat aattacatta caaaatcaga agcacaagcc ctcggctggg
      241 tggcatcaaa agggaacctt gcagacgtcg ctccggggaa aagcatcggc ggagacatct
      301 tctcaaacag ggaaggcaaa ctcccgggca aaagcggacg aacatggcgt gaagcggata
      361 ttaactatac atcaggcttc agaaattcag accggattct ttactcaagc gactggctga
      421 tttacaaaac aacggaccat tatcagacct ttacaaaaat cagataacga aaaaaacggc
      481 ttccctgcgg gaggccgttt ttttcagctt tacataaagt gtgtaataaa tttttcttca
      541 aactctgatc ggtcaatttc actttccggc tctagagctc tagagtccgg tccaatctgc
      601 agccgtccga gacaggagga catcgtccag ct
//
