LOCUS       sequence-172898-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  [Edit] Expresses GFP with +15 theoretical charge in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Thompson DB, Villasenor R, Dorr BM, Zerial M, Liu DR
  TITLE     Cellular uptake mechanisms and endosomal trafficking of supercharged
            proteins.
  JOURNAL   Chem Biol. 2012 Jul 27;19(7):831-43. doi: 
            10.1016/j.chembiol.2012.06.014.
  PUBMED    22840771
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2012.06"; journalName: "Chem Biol"; date: 
            "2012-07-27- 27"; volume: "19"; issue: "7"; pages: "831-43"
COMMENT     Sequence Label: pET-6xHis-(pos15)GFP
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtcatc accaccacca tcacggtggc gctagcaaag gtgaacgtct gtttacgggt
       61 gtagtaccga tcttagtgga attagacggc gacgtgaacg gtcacaaatt tagcgtgcgc
      121 ggcgaaggcg aaggtgacgc tacccgtggt aaattgaccc tgaagtttat ttgcacaaca
      181 ggcaaattac ccgttccgtg gcccacctta gtgaccaccc tgacctatgg cgttcagtgc
      241 ttcagtcgtt accctaaaca tatgaaacgt cacgattttt tcaaatcagc catgcctgaa
      301 ggatatgttc aagagcgtac aatcagcttc aagaaggatg gcacctataa aacgcgtgcg
      361 gaagtgaaat ttgaaggccg cacattagta aaccgtatcg aactgaaagg tcgtgacttc
      421 aaagaaaaag gcaacatttt aggccataag ctggaatata actttaattc tcataacgtg
      481 tatattacgg ccgataaacg caagaatggt atcaaggcaa atttcaaaat tcgccataac
      541 gtgaaagacg gcagcgttca attagcggat cattatcaac aaaacacgcc gattggtcgc
      601 gggcctgtac tgttacctcg caaccactac ctgagcaccc gttcagcact gagcaaagat
      661 ccgaaagaaa aacgcgatca catggttctg ttagaattcg tgaccgctgc aggcattact
      721 cacggaatgg acgaactcta caag
//
