LOCUS       sequence-172903-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP with -27 theoretical charge fused to Cre in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  TITLE     pET-(negative27)GFP-Cre
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-(neg27)GFP-GGS9-Cre-6xHis
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..1113
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             1123..1140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accggtggta gcggtggttc tggtggttct ggtggtagcg gcggtagcgg tggtagcggt
       61 ggtagcggtg gcagcggcgg taccgcgagc aatttactga ccgtacacca aaatttgcct
      121 gcattgccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg
      181 gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg
      241 gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc
      301 gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg
      361 ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct
      421 gtttcactgg ttatgcggcg tatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa
      481 caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc
      541 gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta
      601 cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga
      661 atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca
      721 cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat
      781 gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc
      841 accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt
      901 tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt
      961 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct
     1021 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca
     1081 ggggcaatgg tgcgcctgct ggaagatggc gacggcggat cccatcacca ccaccatcac
     1141 taatga
//
