LOCUS       sequence-172911-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             616..648
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    662..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aaagaactta tcaagtgtgt ggtggtggga gacggagctg taggtaaaac ttgcctactg
       61 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat
      121 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga
      181 ctagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt
      241 tgcttttccc ttgtgagtcc tgcatcattc caccacgtcc gtgcaaagtg gtatcctgag
      301 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg
      361 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg
      421 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc
      481 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct
      541 cccgtgaaga agaggaagag aaaatgcctg ctgttgtaaa agcttgcggc cgcacagctg
      601 tatacacgtg caagccagcc agaactcgcc ccggaagacc ccgaggatct cgagtgtgga
      661 attgtgagcg gataacaatt
//
