LOCUS       sequence-17292-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hartley RW
  TITLE     Directed mutagenesis and barnase-barstar recognition.
  JOURNAL   Biochemistry 1993 Jun 15;32(23):5978-84.
  PUBMED    8507637
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry"; date: "1993-06-15"; volume: "32(23)"; pages: 
            "5978-84"
COMMENT     Sequence Label: pMT643
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(447..463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    471..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(495..501,502..519,520..525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctactcccca tccccctgtt gacattaatc atcggctcgt ataatgtgtg gaattgtgag
       61 cggataacaa tttcacacag gaaacaggat ccgtcataag aaaggagccg cacatgaaaa
      121 aagcagtcat taacggggaa caaatcagaa gtatcagcga cctccaccag acattgaaaa
      181 aggagcttgc ccttccggaa tactacggtg aaaacctgga cgctttatgg gatgcgctga
      241 ccggatgggt ggagtacccg ctcgttttgg aatggaggca gtttgaacaa agcaagcagc
      301 tgactgaaaa tggcgccgag agtgtgcttc aggttttccg tgaagcgaaa gcggaaggcg
      361 cggacatcac catcatactt tcttaatacg atcaatggga gatgaacaat atggaaacac
      421 aaacccgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga ac
//
