LOCUS       sequence-172940-        7417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing TaCas9-nos terminator between attL1 and 
            attR5 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Gil-Humanes J, Wang Y, Liang Z, Shan Q, Ozuna CV, Sanchez-Leon S, 
            Baltes NJ, Starker C, Barro F, Gao C, Voytas DF
  TITLE     High-efficiency gene targeting in hexaploid wheat using DNA 
            replicons and CRISPR/Cas9.
  JOURNAL   Plant J. 2016 Dec 10. doi: 10.1111/tpj.13446.
  PUBMED    27943461
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Dec 10. doi: 10.1111/tpj.13446."
COMMENT     Sequence Label: pJG80
FEATURES             Location/Qualifiers
     source          1..7417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     374..393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     627..644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(803..830)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(922..1008)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1057..1079
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1071..1088
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1072..1088
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1104..1203
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1234..1299
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1306..1326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1351..5451
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1351..5451
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5452..5499
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5510..5762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5807..5930
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5947..5966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5948..5966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5971..5987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5971..5987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6418..7209
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(7302..7417,1..473)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
       61 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgcgagcc
      781 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
      841 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
      901 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      961 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     1021 tccctactct cgcgttaacg cttgcatgga tgttttccca gtcacgacgt tgtaaaacga
     1081 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
     1141 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
     1201 gctccgaatt cgcccttcac catggcttct agcgactaca aggaccacga cggggactac
     1261 aaggaccacg acatcgacta caaggacgac gacgacaaga tggctccaaa gaagaagagg
     1321 aaggttggca tccacggggt gccggctgct gacaagaagt actcgatcgg cctcgacatc
     1381 gggacgaact cagttggctg ggccgtgatc accgacgagt acaaggtgcc ctctaagaag
     1441 ttcaaggtcc tggggaacac cgaccgccat tccatcaaga agaacctcat cggcgctctc
     1501 ctgttcgaca gcggggagac cgctgaggct acgaggctca agagaaccgc taggcgccgg
     1561 tacacgagaa ggaagaacag gatctgctac ctccaagaga ttttctccaa cgagatggcc
     1621 aaggttgacg attcattctt ccaccgcctg gaggagtctt tcctcgtgga ggaggataag
     1681 aagcacgagc ggcatcccat cttcggcaac atcgtggacg aggttgccta ccacgagaag
     1741 taccctacga tctaccatct gcggaagaag ctcgtggact ccaccgataa ggcggacctc
     1801 agactgatct acctcgctct ggcccacatg atcaagttcc gcggccattt cctgatcgag
     1861 ggggatctca acccagacaa cagcgatgtt gacaagctgt tcatccaact cgtgcagacc
     1921 tacaaccaac tcttcgagga gaacccgatc aacgcctctg gcgtggacgc gaaggctatc
     1981 ctgtccgcga ggctctcgaa gtccaggagg ctggagaacc tgatcgctca gctcccaggc
     2041 gagaagaaga acggcctgtt cgggaacctc atcgctctca gcctggggct caccccgaac
     2101 ttcaagtcga acttcgatct cgctgaggac gccaagctgc aactctccaa ggacacctac
     2161 gacgatgacc tcgataacct cctggcccag atcggcgatc aatacgcgga cctgttcctc
     2221 gctgccaaga acctgtcgga cgccatcctc ctgtcagata tcctccgcgt gaacaccgag
     2281 atcacgaagg ctccactctc tgcctccatg atcaagcgct acgacgagca ccatcaggat
     2341 ctgaccctcc tgaaggcgct ggtccgccaa cagctcccgg agaagtacaa ggagattttc
     2401 ttcgatcagt cgaagaacgg ctacgctggg tacatcgacg gcggggcctc acaagaggag
     2461 ttctacaagt tcatcaagcc aatcctggag aagatggacg gcacggagga gctcctggtg
     2521 aagctcaaca gggaggacct cctgcggaag cagagaacct tcgataacgg cagcatcccc
     2581 caccaaatcc atctcgggga gctgcacgcc atcctgagaa ggcaagagga cttctaccct
     2641 ttcctcaagg ataaccggga gaagatcgag aagatcctga ccttcagaat cccatactac
     2701 gtcggccctc tcgcgcgggg gaactcaaga ttcgcttgga tgacccgcaa gtctgaggag
     2761 accatcacgc cgtggaactt cgaggaggtg gtggacaagg gcgctagcgc tcagtcgttc
     2821 atcgagagga tgaccaactt cgacaagaac ctgcccaacg agaaggtgct ccctaagcac
     2881 tcgctcctgt acgagtactt caccgtctac aacgagctca cgaaggtgaa gtacgtcacc
     2941 gagggcatgc gcaagccagc gttcctgtcc ggggagcaga agaaggctat cgtggacctc
     3001 ctgttcaaga ccaaccggaa ggtcacggtt aagcaactca aggaggacta cttcaagaag
     3061 atcgagtgct tcgattcggt cgagatcagc ggcgttgagg accgcttcaa cgccagcctc
     3121 gggacctacc acgatctcct gaagatcatc aaggataagg acttcctgga caacgaggag
     3181 aacgaggata tcctggagga catcgtgctg accctcacgc tgttcgagga cagggagatg
     3241 atcgaggagc gcctgaagac gtacgcccat ctcttcgatg acaaggtcat gaagcaactc
     3301 aagcgccgga gatacaccgg ctgggggagg ctgtcccgca agctcatcaa cggcatccgg
     3361 gacaagcagt ccgggaagac catcctcgac ttcctgaaga gcgatggctt cgccaacagg
     3421 aacttcatgc aactgatcca cgatgacagc ctcaccttca aggaggatat ccaaaaggct
     3481 caagtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc tggctccccc
     3541 gcgatcaaga agggcatcct ccagaccgtg aaggttgtgg acgagctcgt gaaggtcatg
     3601 ggccggcaca agcctgagaa catcgtcatc gagatggcca gagagaacca aaccacgcag
     3661 aaggggcaaa agaactctag ggagcgcatg aagcgcatcg aggagggcat caaggagctg
     3721 gggtcccaaa tcctcaagga gcacccagtg gagaacaccc aactgcagaa cgagaagctc
     3781 tacctgtact acctccagaa cggcagggat atgtacgtgg accaagagct ggatatcaac
     3841 cgcctcagcg attacgacgt cgatcatatc gttccccagt ctttcctgaa ggatgactcc
     3901 atcgacaaca aggtcctcac caggtcggac aagaaccgcg gcaagtcaga taacgttcca
     3961 tctgaggagg tcgttaagaa gatgaagaac tactggaggc agctcctgaa cgccaagctg
     4021 atcacgcaaa ggaagttcga caacctcacc aaggctgaga gaggcgggct ctcagagctg
     4081 gacaaggccg gcttcatcaa gcggcagctg gtcgagacca gacaaatcac gaagcacgtt
     4141 gcgcaaatcc tcgactctcg gatgaacacg aagtacgatg agaacgacaa gctgatcagg
     4201 gaggttaagg tgatcaccct gaagtctaag ctcgtctccg acttcaggaa ggatttccag
     4261 ttctacaagg ttcgcgagat caacaactac caccatgccc atgacgctta cctcaacgct
     4321 gtggtcggca ccgctctgat caagaagtac ccaaagctgg agtccgagtt cgtgtacggg
     4381 gactacaagg tttacgatgt gcgcaagatg atcgccaagt cggagcaaga gatcggcaag
     4441 gctaccgcca agtacttctt ctactcaaac atcatgaact tcttcaagac cgagatcacg
     4501 ctggccaacg gcgagatccg gaagagaccg ctcatcgaga ccaacggcga gacgggggag
     4561 atcgtgtggg acaagggcag ggatttcgcg accgtccgca aggttctctc catgccccag
     4621 gtgaacatcg tcaagaagac cgaggtccaa acgggcgggt tctcaaagga gtctatcctg
     4681 cctaagcgga acagcgacaa gctcatcgcc agaaagaagg actgggaccc aaagaagtac
     4741 ggcgggttcg acagccctac cgtggcctac tcggtcctgg ttgtggcgaa ggttgagaag
     4801 ggcaagtcca agaagctcaa gagcgtgaag gagctcctgg ggatcaccat catggagagg
     4861 tccagcttcg agaagaaccc aatcgacttc ctggaggcca agggctacaa ggaggtgaag
     4921 aaggacctga tcatcaagct cccgaagtac tctctcttcg agctggagaa cggcaggaag
     4981 agaatgctgg cttccgctgg cgagctccag aaggggaacg agctcgcgct gccaagcaag
     5041 tacgtgaact tcctctacct ggcttcccac tacgagaagc tcaagggcag cccggaggac
     5101 aacgagcaaa agcagctgtt cgtcgagcag cacaagcatt acctcgacga gatcatcgag
     5161 caaatctccg agttcagcaa gcgcgtgatc ctcgccgacg cgaacctgga taaggtcctc
     5221 tccgcctaca acaagcaccg ggacaagccc atcagagagc aagcggagaa catcatccat
     5281 ctcttcaccc tgacgaacct cggcgctcct gctgctttca agtacttcga caccacgatc
     5341 gatcggaaga gatacacctc cacgaaggag gtcctggacg cgaccctcat ccaccagtcg
     5401 atcaccggcc tgtacgagac gaggatcgac ctctcacaac tcggcgggga taagagaccc
     5461 gcagcaacca agaaggcagg gcaagcaaag aagaagaagt gagacgtccg atcgttcaaa
     5521 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5581 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5641 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5701 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5761 tcgggaattg atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca
     5821 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     5881 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg ccatccagct
     5941 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     6001 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     6061 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     6121 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     6181 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     6241 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     6301 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     6361 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     6421 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     6481 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     6541 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     6601 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     6661 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     6721 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     6781 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     6841 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     6901 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     6961 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     7021 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     7081 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     7141 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     7201 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     7261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7381 gatcaagagc taccaactct ttttccgaag gtaactg
//
