LOCUS       sequence-172947-        5610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing HA-C1orf27-IRES-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs-HA-C1orf27
FEATURES             Location/Qualifiers
     source          1..5610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(280..297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(451..1039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(531..550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1203..1994,1995..2063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1826..1845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2064..2168)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2198..2790
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2853..3210
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3269..3685
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3615..3637
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3653..3669
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3695..4069
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     4007..4023
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             4107..4133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5532..5597
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
       61 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
      121 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
      181 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
      241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1141 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     1201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2161 gttccgcgca catttccccg aaaagtgcca cctgcataat gaaagacccc acctgtaggt
     2221 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     2281 tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     2341 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     2401 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     2461 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     2521 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     2581 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctcc
     2641 tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     2701 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     2761 ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct
     2821 gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg
     2881 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
     2941 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
     3001 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa
     3061 tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg tggttctggt
     3121 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     3181 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     3241 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     3301 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     3361 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     3421 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     3481 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     3541 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     3601 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     3661 agccctcact ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc
     3721 cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct
     3781 ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc
     3841 agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac
     3901 agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac
     3961 agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc
     4021 cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc cggatctagc
     4081 tagttaatta aggatccgcc accatgtatc cgtatgatgt gccggattat gcgatgggaa
     4141 gaacctacat tgtagaagag actgttggcc agtatctttc aaacataaat ctccaaggaa
     4201 aggcttttgt ctctggcctt ttaataggac agtgttcgtc acaaaaggat tatgtgattc
     4261 ttgccactag aacgccaccc aaagaggagc aaagtgagaa cctcaaacat cccaaagcta
     4321 agttggataa cttggatgaa gaatgggcca cagaacatgc ctgccaggta tccagaatgc
     4381 taccaggggg acttttagtt cttggagtat ttattattac tactttagaa ctggcaaatg
     4441 attttcaaaa tgccctgcgt agactaatgt ttgctgtgga aaagtctata aatagaaaga
     4501 gattgtggaa tttcacagag gaggaagtct cagaacgagt gacacttcac atttgtgctt
     4561 ctacaaaaaa aatattttgt cgaacttatg atatccatga tccaaagagt tcagcaagac
     4621 cagcagattg gaagtatcaa agtggattat catcctcatg gctttcttta gagtgtacag
     4681 ttcacattaa tattcacatc ccactttctg ctacttctgt cagctatact ctggagaaaa
     4741 atacaaagaa tggacttaca cgctgggcca aggaaataga aaatggtgtt tatttgatta
     4801 atggacaagt taaagatgaa gattgtgacc tattagaagg acagaaaaaa tcttctagag
     4861 gaaatactca agcaactagt cattcttttg atgtcagagt gctaacgcag ttgctcctga
     4921 attcagacca cagatccaca gccacagtcc agatatgtag cggttctgta aaccttaagg
     4981 gtgctgtgaa atgcagagct tatatccaca gcagtaaacc caaagttaaa gatgctgtgc
     5041 aggcagtaaa gagggatata ttgaacacag ttgctgatcg ttgtgaaatg ctatttgagg
     5101 atctgctttt gaatgaaatt ccagaaaaaa aagattctga aaaagagttc cacgtcctcc
     5161 cttatcgagt ctttgttccc cttcctggat ccactgtaat gttgtgtgat tataaatttg
     5221 acgatgagtc agctgaagaa atcagggacc attttatgga gatgttggat cacacaattc
     5281 aaatagaaga tttggaaatt gcagaggaaa caaacacagc ttgtatgagt tcttctatga
     5341 atagtcaagc ttcattggac aacacagatg atgaacaacc aaaacaacca attaaaacta
     5401 caatgttatt gaaaattcag caaaacatag gtgtgattgc agcatttaca gttgcagtcc
     5461 ttgctgcggg tatctccttt cattacttca gtgattaggc ggccgcagga ggcggtggag
     5521 gcggtgcagg cgactacaaa gaccatgacg gtgattataa agatcatgac attgattaca
     5581 aggatgacga tgacaagtaa taataaattc
//
