LOCUS       sequence-172948-        7183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expressing HA-C1orf27-IRES-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs-HA-C1orf27
FEATURES             Location/Qualifiers
     source          1..7183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..614
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    677..1034
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1093..1509
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1439..1461
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1477..1493
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1519..1893
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1831..1847
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1931..1957
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3356..3421
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(3460..4034,4035..4037,4038..4046)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3627..3644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3854..3873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4047..4442
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4688..5157
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5287..5304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5458..6046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5538..5557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6210..7001,7002..7070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6833..6852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7071..7175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcctcggcg cgccagtcct
      481 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      541 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      601 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      661 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      721 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      781 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca
      841 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga
      901 ctctttggtg caccccccta ataggaggga tatgtggttc tggtaggaga cgagaaccta
      961 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg
     1021 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     1081 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     1141 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     1201 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     1261 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     1321 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     1381 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
     1441 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
     1501 ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac
     1561 ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag
     1621 gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa
     1681 ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga
     1741 caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc
     1801 cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct
     1861 gccgaccccg ggggtggacc atcctctaga ctgccggatc tagctagtta attaaggatc
     1921 cgccaccatg tatccgtatg atgtgccgga ttatgcgatg ggaagaacct acattgtaga
     1981 agagactgtt ggccagtatc tttcaaacat aaatctccaa ggaaaggctt ttgtctctgg
     2041 ccttttaata ggacagtgtt cgtcacaaaa ggattatgtg attcttgcca ctagaacgcc
     2101 acccaaagag gagcaaagtg agaacctcaa acatcccaaa gctaagttgg ataacttgga
     2161 tgaagaatgg gccacagaac atgcctgcca ggtatccaga atgctaccag ggggactttt
     2221 agttcttgga gtatttatta ttactacttt agaactggca aatgattttc aaaatgccct
     2281 gcgtagacta atgtttgctg tggaaaagtc tataaataga aagagattgt ggaatttcac
     2341 agaggaggaa gtctcagaac gagtgacact tcacatttgt gcttctacaa aaaaaatatt
     2401 ttgtcgaact tatgatatcc atgatccaaa gagttcagca agaccagcag attggaagta
     2461 tcaaagtgga ttatcatcct catggctttc tttagagtgt acagttcaca ttaatattca
     2521 catcccactt tctgctactt ctgtcagcta tactctggag aaaaatacaa agaatggact
     2581 tacacgctgg gccaaggaaa tagaaaatgg tgtttatttg attaatggac aagttaaaga
     2641 tgaagattgt gacctattag aaggacagaa aaaatcttct agaggaaata ctcaagcaac
     2701 tagtcattct tttgatgtca gagtgctaac gcagttgctc ctgaattcag accacagatc
     2761 cacagccaca gtccagatat gtagcggttc tgtaaacctt aagggtgctg tgaaatgcag
     2821 agcttatatc cacagcagta aacccaaagt taaagatgct gtgcaggcag taaagaggga
     2881 tatattgaac acagttgctg atcgttgtga aatgctattt gaggatctgc ttttgaatga
     2941 aattccagaa aaaaaagatt ctgaaaaaga gttccacgtc ctcccttatc gagtctttgt
     3001 tccccttcct ggatccactg taatgttgtg tgattataaa tttgacgatg agtcagctga
     3061 agaaatcagg gaccatttta tggagatgtt ggatcacaca attcaaatag aagatttgga
     3121 aattgcagag gaaacaaaca cagcttgtat gagttcttct atgaatagtc aagcttcatt
     3181 ggacaacaca gatgatgaac aaccaaaaca accaattaaa actacaatgt tattgaaaat
     3241 tcagcaaaac ataggtgtga ttgcagcatt tacagttgca gtccttgctg cgggtatctc
     3301 ctttcattac ttcagtgatt aggcggccgc aggaggcggt ggaggcggtg caggcgacta
     3361 caaagaccat gacggtgatt ataaagatca tgacattgat tacaaggatg acgatgacaa
     3421 gtaataataa attccgcccc cccccccccc cccccccccc ccctctccct cccccccccc
     3481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3541 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3601 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3661 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3721 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3781 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3841 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3901 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3961 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4021 aacacgatga taatatggcc acaaccatgg ttcctttgtc tcaagaagaa tccaccctca
     4081 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4141 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     4201 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     4261 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     4321 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     4381 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     4441 aagtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     4501 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     4561 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     4621 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     4681 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     4741 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     4801 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     4861 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     4921 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     4981 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     5041 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     5101 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat
     5161 gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata
     5221 gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6121 gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa
     6181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7141 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttc
//
