LOCUS       sequence-172949-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272,
            in M15 cells already pre-transformed with 2nd plasmid, pREP-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Poon GM
  TITLE     Sequence discrimination by DNA-binding domain of ETS family 
            transcription factor PU.1 is linked to specific hydration of 
            protein-DNA interface.
  JOURNAL   J Biol Chem. 2012 May 25;287(22):18297-307. doi: 
            10.1074/jbc.M112.342345. Epub 2012 Apr 2.
  PUBMED    22474303
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.342345"; journalName: "J Biol Chem"; date: 
            "2012-05-25- 25"; volume: "287"; issue: "22"; pages: "18297-307"
COMMENT     Sequence Label: pQE60-mPU.1-ETS
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..49
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(73..87,88..93,94..110,111..117)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             160..171
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             499..516
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      563..658
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(773..792)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cnnntnntta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
       61 cacctcgaga aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat
      121 tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa attaaccatg
      181 ggcagcaaga agaagattcg tctgtaccaa ttcctgctcg atttgctgcg gtcaggagat
      241 atgaaagact cgatctggtg ggtcgataaa gacaaaggta cctttcaatt cagttctaaa
      301 cataaagagg cattagcgca caggtgggga attcagaagg gcaatcgcaa gaagatgaca
      361 taccagaaga tggcgcgtgc tctgcgcaac tatggcaaga ctggtgaagt taagaaagtg
      421 aagaagaaat tgacgtatca gtttagcggc gaagtattag ggcgtggtgg gctggccgaa
      481 agacgccttc cgccacatct agtgcctcga ggttccagat ctcatcacca tcaccatcac
      541 taagcttaat tagctgagct tggactcctg ttgatagatc cggtaatgac ctcagaactc
      601 catctggatt tgttcagaac gctcggttgc ccgccgggcg ttttttattg gtgagaatcc
      661 aagctagctt ggcgagattt tcaggagcta aggaagctaa aaatggaaaa aaaaaatcac
      721 tgggatatac caccgttgat atatcgccaa tggcatcgta aagaacattt ttgaggcatt
      781 tcagtcagtt gctcagtgta gctataagcg agacggttca cctggaatat aacggccttt
      841 ttaaagacca tagagaaaag ataagcggag gttttatcgg gcttttattc accatgcttg
      901 cccgcctgga tgaatggctc atccgaaatt tcggatgagc agtgg
//
