LOCUS       sequence-17295-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Hartley RW, Both V, Hebert EJ, Homerova D, Jucovic M, Nazarov V, 
            Rybajlak I, Sevcik J
  TITLE     Barstar inhibits extracellular ribonucleases of Streptomyces and 
            allows their production from recombinant genes.
  JOURNAL   Protein Peptide Letters 1996 3:225-231
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Peptide Letters 1996 3:225-231"
COMMENT     Sequence Label: pMT1002
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     complement(251..313)
                     /codon_start=1
                     /product="signal sequence of E. coli alkaline phosphatase 
                     (Inouye et al., 1982)"
                     /label=phoA signal sequence
                     /translation="MKQSTIALALLPLLFTPVTKA"
     RBS             339..347
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cncnatctga gctgagtata gttatatccg cttcacgcca tgttcgtccg cttttgcccg
       61 ggagtttgcc ttccctgttt gagaagatgt ctccgccgat gcttttcccc ggagcgacgt
      121 ctgcaaggtt cccttttgat gccacccagc cgagggcttg tgcttctgat tttgtaatgt
      181 aattatcagg tagcttatga tatgtctgaa gataatccgc aaccccgtca aacgtgttga
      241 taacctgtgc ggcttttgtc acaggggtaa acagtaacgg taagagtgcc agtgcaatag
      301 tgctttgttt cactttattt tctccacgga tccatacaac ctccttagta catgcaacca
      361 ttatcaccgc cagaggtaaa atagtcaaca cgcacggtgt tagatattta tcccttgcgg
      421 tgatagattt aacgtatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac
      481 gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa
      541 tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc
      601 aatgcattaa atgcttataa cgccgcattg cttacaaaaa ttctcaangt tagcgttgaa
      661 gaatttagcc cttcaatcgc cagagaaatc tacgagatgt
//
