LOCUS       sequence-172950-        5665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of chicken CAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Patzke C, Max KE, Behlke J, Schreiber J, Schmidt H, Dorner AA, 
            Kroger S, Henning M, Otto A, Heinemann U, Rathjen FG
  TITLE     The coxsackievirus-adenovirus receptor reveals complex homophilic 
            and heterophilic interactions on neural cells.
  JOURNAL   J Neurosci. 2010 Feb 24;30(8):2897-910. doi: 
            10.1523/JNEUROSCI.5725-09.2010.
  PUBMED    20181587
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5725-09"; journalName: "J Neurosci"; date: 
            "2010-02-24- 24"; volume: "30"; issue: "8"; pages: "2897-910"
COMMENT     Sequence Label: pSG5-chCAR
FEATURES             Location/Qualifiers
     source          1..5665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..242
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        54..250
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      101..236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          281..853
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     861..880
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     908..927
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        908..926
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2558..2679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2595..2614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2649..2668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2713..2730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2884..3472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2964..2983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3643..4434,4435..4503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4266..4285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4504..4608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4676..4694
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4732..4754)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4854..4873
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4940..5395)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5077..5098)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5289..5308
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5521..5543
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5535..5552
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5536..5552
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5589..5609)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tagtcagcaa ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
       61 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      121 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      181 ggccgcctcg gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggcctagg
      241 cttttgcaaa aagctccgga tcgatcctga gaacttcagg gtgagtttgg ggacccttga
      301 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
      361 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
      421 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
      481 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
      541 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
      601 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
      661 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
      721 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
      781 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
      841 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
      901 agaattgtaa tacgactcac tatagggcga attcggcacg agcggcacga gcgagccgcg
      961 ctatggaacc gccgccgttg gtgctgctgg tggggctggc gctgctcggt gccgcaggtc
     1021 ttacaagaag cctaagtata acttcagctg aatcagcatt tgaaaaagct caaggagaga
     1081 gagttacgtt gccatgtacc tttgaactct cagaagagga tgtaggcaca ctagatattg
     1141 agtgggtttt gataccagca gatattcaga agaaggaaga aacaataatt ctatattctg
     1201 gagataggat ttataatcat tatcatcctg ctctggctgg gcggctgcaa tttactagtt
     1261 ctgatcccaa atctggtgat ggttcagtgg atatcctgaa tttaaagtca gcagatactg
     1321 gcacatacca gtgcaaagtg aagaaggctc ctggagttga aagcctaaaa atacagttga
     1381 acgtacttgt aaagccagca agcactaaat gctccattga aggatcacag gagattggaa
     1441 aggacattgt attgaagtgt gcatcacaag aaggaacccc acttttgtat tatgactgga
     1501 gaagagtagt aactggcaca caaggacttc ctgccacttc cgtactaaac aaaaatacag
     1561 gggaacttct cttgaaaaat gcctctaaag actattctgg tacatacagt tgtgttgctt
     1621 caaaccgagt tggcacagat gaatgttctg ttgagctgaa tgtcacccct cctataaata
     1681 cagctggtgt aattgctgga gctattctgg gaactctgtt gggtcttgcc ttgttggctt
     1741 ttcttgtcat ctgttgctgt aagaagcata gagagaagaa atatgagaaa gaagtacatc
     1801 atgaaatcag agaagatgtt ctgcctccaa aaagtcgcag ttcaacagca cgcagctaca
     1861 taggcagcaa tcgttcttct ctgggttcaa tgtctccctc aaatatggaa ggatactcca
     1921 aaactccata tagccaggtt ccaagtgaag actttgaacg tacttctggt caaaaccaaa
     1981 ccattgcatc ttcaaaggta gctgcaccta atttaagtag aatgggagct gtccctgtga
     2041 tgattccagc acaaagcaaa gatgggtcca tagtatgaaa tattaattta agtcctgggt
     2101 tttttaagtg tttgttgtaa gtattagaga aaactacagt attccaaccc tcatttaaac
     2161 aatggcatgc aattttcctt gaagtaaatg aacatgttag tttgaaaagc caccaattct
     2221 catttttaat tttaaactta ttagtgtgta acagttgaac tattgaaagc gtgagagttc
     2281 ctaaatatca gatactgaag gtgtttggat ctctggtggc ttgctgaaga gatgctatta
     2341 gctgatgtgc agttctcaga atctgaaaga gcaacacaac tgagaagtaa caaaaccatt
     2401 tcatatgtag acaatgaagt tcctcagagt tcctgaagct ttattctcag tcttggagta
     2461 aaggggtatt ttaatttggc cactgtctga tgaacttgca gtggggctga gatactgagg
     2521 agaactttga attcggatcc agatcttatt aaagcagaac ttgtttattg cagcttataa
     2581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgactctaga
     2701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3301 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4621 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4861 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4921 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4981 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     5041 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     5101 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     5161 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     5221 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     5281 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5341 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
     5401 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5461 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     5521 cccagtcacg acgttgtaaa acgacggcca gtgaattgtc gacttctgag gcggaaagaa
     5581 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5641 aagtatgcaa agcatgcatc tcaat
//
