LOCUS       sequence-172957-        2254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs2-RAC1
FEATURES             Location/Qualifiers
     source          1..2254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..453
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     383..405
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     421..437
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    463..837
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     775..791
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      866..875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             875..940
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1535..1537,1538..1540,1541..2251)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1535..1537,1538..1540,1541..2251)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1580..1601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1841..1860)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2188..2209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
       61 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
      121 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
      181 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
      241 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
      301 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
      361 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
      421 ctttatccag ccctcactcc ttctctaggc gcccccatat ggccatatga gatcttatat
      481 ggggcacccc cgccccttgt aaacttccct gaccctgaca tgacaagagt tactaacagc
      541 ccctctctcc aagctcactt acaggctctc tacttagtcc agcacgaagt ctggagacct
      601 ctggcggcag cctaccaaga acaactggac cgaccggtgg tacctcaccc ttaccgagtc
      661 ggcgacacag tgtgggtccg ccgacaccag actaagaacc tagaacctcg ctggaaagga
      721 ccttacacag tcctgctgac cacccccacc gccctcaaag tagacggcat cgcagcttgg
      781 atacacgccg cccacgtgaa ggctgccgac cccgggggtg gaccatcctc tagactgccg
      841 gatctagcta gttaattaag gatccgccac catggactat aaggaccatg atggagatta
      901 taaggaccac gatattgact ataaagacga tgacgacaaa gggggccagg caatcaagtg
      961 tgtggtggtt ggagatggag ccgtgggcaa gacctgtttg ctgatttctt acacaaccaa
     1021 tgcgttcccc ggcgaataca ttccgacagt tttcgataac tacagcgcaa atgttatggt
     1081 ggacgggaaa ccggtgaatc tgggactctg ggatactgcc ggccaggagg actacgatcg
     1141 cctccggccg ctctcctatc ctcagacaga tgtgttcctc atctgcttct cactcgtatc
     1201 cccagcaagt ttcgagaacg ttcgggctaa gtggtacccc gaagtacgcc accactgtcc
     1261 taatacccca atcattctcg tcgggactaa gctcgatctg cgggatgata aagacacgat
     1321 cgagaaattg aaggagaaaa aactcacacc tatcacatac ccccagggtc tggcaatggc
     1381 taaggagata ggcgctgtga aatacttgga gtgctctgcc cttacacaga gaggcctgaa
     1441 aactgtgttt gatgaggcca ttcgagcagt cctgtgtccg ccccccgtta agaagaggaa
     1501 acggaagtgt ttgttgctgt aggcggccgc aggcatggtg agcaagggcg aggagctgtt
     1561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1621 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1741 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2221 gatcactctc ggcatggacg agctgtacaa gggc
//
