LOCUS       sequence-172958-        2585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs2-RAC1
FEATURES             Location/Qualifiers
     source          1..2585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    54..516
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(110..127)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     337..356
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             530..922
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             950..1006
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1007..1720
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     LTR             1900..2369
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2499..2516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
       61 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      121 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
      181 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
      241 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
      301 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
      361 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
      421 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
      481 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggttccttt
      541 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
      601 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
      661 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
      721 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
      781 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
      841 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
      901 ctctggttat gtgtgggagg gcggaggtgg tggagctagc ggcagcggcg ccaccaactt
      961 cagcctgctg aagcaggccg gcgacgtgga ggagaacccc ggccccatgg tgtctaaggg
     1021 cgaagagctg attaaggaga acatgcacat gaagctgtat atggagggca ccgtgaacaa
     1081 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     1141 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
     1201 cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa
     1261 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     1321 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     1381 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     1441 ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc
     1501 cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa
     1561 gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag
     1621 aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata
     1681 ctgcgacctc cctagcaaac tggggcacaa acttaattga acgcgttaag tcgacgataa
     1741 aataaaagat tttattttgt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     1801 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     1861 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     1921 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     1981 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     2041 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     2101 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     2161 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     2221 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     2281 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     2341 attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt
     2401 tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg
     2461 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2521 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2581 acaga
//
