LOCUS       sequence-172968-       11576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for generating green fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Gg
FEATURES             Location/Qualifiers
     source          1..11576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             43..1116
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1182..1376
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1612..1634)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1720..1860
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1775..1794
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1875..1892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2046..2631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2123..2142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2718..3512)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3937..3961
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4056..4850)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4167..4186)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4777..4796
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     intron          4859..4974
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     protein_bind    5816..5940
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5965..5970,5971..5988,5989..5995)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6049..6708
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6115..6134)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7050..7355
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7234..7253
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7396..7520)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(7540..7542,7543..7545,7546..8259)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7540..7542,7543..7545,7546..8256)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7585..7606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7846..7865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8193..8214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      8413..8984
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(9017..9034)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9017..9033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9026..9048)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9236..9260
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10561..11190
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
       61 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
      121 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
      181 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
      241 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
      301 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
      361 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
      421 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
      481 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
      541 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
      601 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
      661 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
      721 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
      781 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
      841 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
      901 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
      961 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     1021 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     1081 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     1141 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     1201 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     1261 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     1321 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     1381 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     1441 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     1501 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     1561 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1621 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     1681 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     1741 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1801 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1861 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1921 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1981 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2041 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2101 aggtggcgaa acccgacagg ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2461 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2701 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
     2761 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     2821 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     2881 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     2941 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     3001 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
     3061 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     3121 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     3181 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     3241 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     3301 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     3361 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     3421 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
     3481 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
     3541 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
     3601 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
     3661 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
     3721 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
     3781 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
     3841 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
     3901 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
     3961 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
     4021 cgccgaatat cgaattcctg cagcccgggg gatcctcaga agaactcgtc aagaaggcga
     4081 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4141 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4201 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4261 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     4321 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4381 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4441 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4501 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4561 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4621 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc
     4681 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     4741 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     4801 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat ggtgatgtct
     4861 gctcaagcgg ggtagctgtt agtcaagctg cgatgaagtg ggaaagctcg aactgaaagg
     4921 ttcaaaggaa taagggatgg gaaggatgga gtatggatgt agcaaagtac ttacttaggg
     4981 gaaataaagg ttcttggatg ggaagatgaa tatactgaag atgggaaaag aaagagaaaa
     5041 gaaaagagca gctggtgggg agagcaggaa aatatggcaa caaatgttgg actgacgcaa
     5101 cgaccttgtc aaccccgccg acacaccggg cggacagacg gggcaaagct gcctaccagg
     5161 gactgaggga cctcagcagg tcgagtgcag agcaccggat gggtcgactg ccagcttgtg
     5221 ttcccggtct gcgccgctgg ccagctcctg agcggccttt ccggtttcat acaccgggca
     5281 aagcaggaga ggcacgatat ttggacgccc tacagatgcc ggatgggcca attagggagc
     5341 ttacgcgccg ggtactcgct ctacctactt cggagaaggt actatctcgt gaatctttta
     5401 ccagatcgga agcaattgga cttctgtacc taggttaatg gcatgctatt tcgccgacgg
     5461 ctatacaccc ctggcttcac attctccttc gcttactgcc ggtgattcga tgaagctcca
     5521 tattctccga tgatgcaata gattcttggt caacgagggg cacaccagcc tttccacttc
     5581 ggggcggagg ggcggccggt cccggattaa taatcatcca ctgcacctca gagccgccag
     5641 agctgtctgg cgcagtggcg cttattactc agcccttctc tctgcgtccg tccgtctctc
     5701 cgcatgccag aaagagtcac cggtcactgt acagagctct cccatatggt cgacctgcag
     5761 gcggccgcga attcactagt gatttataag cttacgaacg atagacatct ggatcacaag
     5821 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     5881 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     5941 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taacgtgtgg
     6001 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     6061 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     6121 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     6181 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     6241 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     6301 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     6361 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     6421 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     6481 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     6541 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     6601 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     6661 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     6721 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     6781 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     6841 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     6901 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     6961 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     7021 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     7081 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     7141 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     7201 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     7261 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     7321 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     7381 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     7441 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     7501 gctttcttgt acaaagtggt gatagggccc gggatcctga tggtgagcaa gggcgaggag
     7561 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7621 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7681 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     7741 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7801 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7861 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7921 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7981 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     8041 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     8101 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     8161 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     8221 gccgggatca ctctcggcat ggacgagctg tacaagtgag atccacttaa cgttactgaa
     8281 atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
     8341 gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
     8401 agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt ttcgggttta
     8461 cctcttccag atacagctca tctgcaatgc attaatgcat tggacctcgc aaccctagta
     8521 cgcccttcag gctccggcga agcagaagaa tagcttagca gagtctattt tcattttcgg
     8581 gagacgagat caagcagatc aacggtcgtc aagagaccta cgagactgag gaatccgctc
     8641 ttggctccac gcgactatat atttgtctct aattgtactt tgacatgctc ctcttcttta
     8701 ctctgatagc ttgactatga aaattccgtc accagcccct gggttcgcaa agataattgc
     8761 actgtttctt ccttgaactc tcaagcctac aggacacaca ttcatcgtag gtataaacct
     8821 cgaaaatcat tcctactaag atgggtatac aatagtaacc atgcatggtt gcctagtgaa
     8881 tgctccgtaa cacccaatac gccggccgaa acttttttac aactctccta tgagtcgttt
     8941 acccagaatg cacaggtaca cttgtttaga ggtaatcctt ctttctagag tcgacctgca
     9001 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9061 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9121 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     9181 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     9241 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     9301 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     9361 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     9421 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     9481 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     9541 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     9601 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     9661 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     9721 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     9781 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     9841 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     9901 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     9961 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
    10021 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
    10081 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
    10141 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
    10201 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
    10261 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
    10321 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
    10381 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
    10441 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
    10501 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
    10561 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
    10621 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
    10681 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
    10741 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
    10801 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
    10861 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
    10921 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
    10981 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
    11041 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
    11101 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
    11161 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
    11221 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
    11281 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
    11341 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
    11401 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
    11461 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
    11521 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccg
//
