LOCUS       sequence-17297-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kapust RB, Tozser J, Fox JD, Anderson DE, Cherry S, Copeland TD, 
            Waugh DS
  TITLE     Tobacco etch virus protease: mechanism of autolysis and rational 
            design of stable mutants with wild-type catalytic proficiency.
  JOURNAL   Protein Eng. 2001 Dec . 14(12):993-1000.
  PUBMED    11809930
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng. 2001 Dec . 14(12):993-1000."
COMMENT     Sequence Label: pRK792
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..39
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             64..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             85..792
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMPKPE
                     EPFQPVKEATQLMN"
     primer_bind     complement(849..866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(849..865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacaataaca ataacaacaa cctcgggatc gagggaaggg gagaaaatct ttattttcaa
       61 ggtcatcatc atcatcatca tcatggagaa agcttgttta aggggccgcg tgattacaac
      121 ccgatatcga gcaccatttg tcatttgacg aatgaatctg atgggcacac aacatcgttg
      181 tatggtattg gatttggtcc cttcatcatt acaaacaagc acttgtttag aagaaataat
      241 ggaacactgt tggtccaatc actacatggt gtattcaagg tcaagaacac cacgactttg
      301 caacaacacc tcattgatgg gagggacatg ataattattc gcatgcctaa ggatttccca
      361 ccatttcctc aaaagctgaa atttagagag ccacaaaggg aagagcgcat atgtcttgtg
      421 acaaccaact tccaaactaa gagcatgtct agcatggtgt cagacactag ttgcacattc
      481 ccttcatctg atggcatatt ctggaagcat tggattcaaa ccaaggatgg gcagtgtggc
      541 agtccattag tatcaactag agatgggttc attgttggta tacactcagc atcgaatttc
      601 accaacacaa acaattattt cacaagcgtg ccgaaaaact tcatggaatt gttgacaaat
      661 caggaggcgc agcagtgggt tagtggttgg cgattaaatg ctgactcagt attgtggggg
      721 ggccataaag ttttcatgcc gaaacctgaa gagccttttc agccagttaa ggaagcgact
      781 caactcatga atcgtcgtcg ccgtcgctaa taaggatcct ctagagtcga cctgcaggca
      841 agcttggcac tggccgtcgt tttaca
//
