LOCUS       sequence-172986-        6343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For  expression of untagged Parkin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
  TITLE     Atg8 family LC3/GABARAP proteins are crucial for 
            autophagosome-lysosome fusion but not autophagosome formation during
            PINK1/Parkin mitophagy and starvation.
  JOURNAL   J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.
  PUBMED    27864321
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-12-19"; volume: "215(6)"; pages: "857-874. Epub 
            2016 Nov 18"
COMMENT     Sequence Label: pBMN Parkin
FEATURES             Location/Qualifiers
     source          1..6343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             711..1180
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1245..1602
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1667..2083
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2013..2035
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2051..2067
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2093..2466
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2404..2420
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             3920..4513
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4644..4661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4815..5403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4895..4914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5577..6343,1..25,26..94))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6200..6219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
       61 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      121 gcatttatca gggttattgt ctcatgacat taacctataa aaataggcgt atcacgaggc
      181 cctttcgtct tcaagaacag ctttgctctt aggagtttcc taatacatcc caaactcaaa
      241 tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta agccagcttt
      301 ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat ttcataaggg
      361 tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa ataaaaatag
      421 ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca gttgacaaca
      481 taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca ttatgccagt
      541 catattaatt actagtcaat tagttgattt ttatttttga catatacatg tgaaagaccc
      601 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
      661 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
      721 gccaaagcgg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
      781 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
      841 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
      901 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
      961 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     1021 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     1081 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     1141 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     1201 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
     1261 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
     1321 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
     1381 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
     1441 tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag aggagggata
     1501 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
     1561 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
     1621 tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc agactgttac
     1681 cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca
     1741 gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt
     1801 taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat
     1861 caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg
     1921 ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
     1981 gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
     2041 tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat ggccatatga
     2101 gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca tgacaagagt
     2161 tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc agcacgaagt
     2221 ctggagacct ctggcggcac gtaccaagaa caactggacc gaccggtggt acctcaccct
     2281 taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc
     2341 tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc
     2401 gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct
     2461 agactgccgg atccatgata gtgtttgtca ggttcaactc cagccatggt ttcccagtgg
     2521 aggtcgattc tgacaccagc atcttccagc tcaaggaggt ggttgctaag cgacaggggg
     2581 ttccggctga ccagttgcgt gtgattttcg cagggaagga gctgaggaat gactggactg
     2641 tgcagaattg tgacctggat cagcagagca ttgttcacat tgtgcagaga ccgtggagaa
     2701 aaggtcaaga aatgaatgca actggaggcg acgaccccag aaacgcggcg ggaggctgtg
     2761 agcgggagcc ccagagcttg actcgggtgg acctcagcag ctcagtcctc ccaggagact
     2821 ctgtggggct ggctgtcatt ctgcacactg acagcaggaa ggactcacca ccagctggaa
     2881 gtccagcagg tagatcaatc tacaacagct tttatgtgta ttgcaaaggc ccctgtcaaa
     2941 gagtgcagcc gggaaaactc agggtacagt gcagcacctg caggcaggca acgctcacct
     3001 tgacccaggg tccatcttgc tgggatgatg ttttaattcc aaaccggatg agtggtgaat
     3061 gccaatcccc acactgccct gggactagtg cagaattttt ctttaaatgt ggagcacacc
     3121 ccacctctga caaggaaaca tcagtagctt tgcacctgat cgcaacaaat agtcggaaca
     3181 tcacttgcat tacgtgcaca gacgtcagga gccccgtcct ggttttccag tgcaactccc
     3241 gccacgtgat ttgcttagac tgtttccact tatactgtgt gacaagactc aatgatcggc
     3301 agtttgttca cgaccctcaa cttggctact ccctgccttg tgtggctggc tgtcccaact
     3361 ccttgattaa agagctccat cacttcagga ttctgggaga agagcagtac aaccggtacc
     3421 agcagtatgg tgcagaggag tgtgtcctgc agatgggggg cgtgttatgc ccccgccctg
     3481 gctgtggagc ggggctgctg ccggagcctg accagaggaa agtcacctgc gaagggggca
     3541 atggcctggg ctgtgggttt gccttctgcc gggaatgtaa agaagcgtac catgaagggg
     3601 agtgcagtgc cgtatttgaa gcctcaggaa caactactca ggcctacaga gtcgatgaaa
     3661 gagccgccga gcaggctcgt tgggaagcag cctccaaaga aaccatcaag aaaaccacca
     3721 agccctgtcc ccgctgccat gtaccagtgg aaaaaaatgg aggctgcatg cacatgaagt
     3781 gtccgcagcc ccagtgcagg ctcgagtggt gctggaactg tggctgcgag tggaaccgcg
     3841 tctgcatggg ggaccactgg ttcgacgtgt agtcgacgat aaaataaaag attttattta
     3901 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     3961 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     4021 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     4081 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     4141 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     4201 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     4261 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     4321 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     4381 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     4441 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     4501 ggggtctttc attcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat
     4561 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4621 gcgtattggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4681 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4741 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4801 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4861 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4921 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4981 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5041 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5101 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5161 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5221 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5281 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5341 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5401 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5461 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5521 aaatgaagtt tgcgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6181 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat ctt
//
