LOCUS       sequence-172988-       10948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenti-mediated expression of Chronos-GFP under the CaMKII promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: FCK-Chronos-GFP
FEATURES             Location/Qualifiers
     source          1..10948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..90)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        75..404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      255..390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     317..336
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        452..499
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             518..892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1022..1143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1059..1078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1113..1132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1205..1227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1216..1232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1240..1246,1247..1264,1265..1270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1285..1306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1423..1440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1594..2182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1674..1693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2353..3144,3145..3213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2976..2995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3214..3318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3393..3412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3584..3963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3965..4163
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4118..4138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4181..4361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4408..4533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5026..5259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5444..5488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5786..5903
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5948..7236
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      7246..7255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8245..8247,8248..8961)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8245..8247,8248..8958)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8287..8308)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8548..8567)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8895..8916
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8986..9574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9039..9059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9457..9468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9577..9593)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9578..9594)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10099..10279
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10305..10322)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10311..10535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10581..10948,1..61)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10668..10687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10878..10899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
       61 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      421 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
      481 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
      541 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      601 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      661 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      721 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      781 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      841 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
      901 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
      961 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1141 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1201 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1261 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1381 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2701 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2761 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2821 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2881 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2941 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3001 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3061 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3121 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3181 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3241 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3301 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3361 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3421 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3481 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3541 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     3601 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3661 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3721 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3781 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3841 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3901 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4141 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     4201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     4801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     4861 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     4921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     4981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5641 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     5701 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     5761 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     5821 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     5881 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     5941 taattaacat tatggcctta ggtcacttca tctccatggg gttcttcttc tgattttcta
     6001 gaaaatgaga tgggggtgca gagagcttcc tcagtgacct gcccagggtc acatcagaaa
     6061 tgtcagagct agaacttgaa ctcagattac taatcttaaa ttccatgcct tgggggcatg
     6121 caagtacgat atacagaagg agtgaactca ttagggcaga tgaccaatga gtttaggaaa
     6181 gaagagtcca gggcagggta catctacacc acccgcccag ccctgggtga gtccagccac
     6241 gttcacctca ttatagttgc ctctctccag tcctaccttg acgggaagca caagcagaaa
     6301 ctgggacagg agccccagga gaccaaatct tcatggtccc tctgggagga tgggtgggga
     6361 gagctgtggc agaggcctca ggaggggccc tgctgctcag tggtgacaga taggggtgag
     6421 aaagcagaca gagtcattcc gtcagcattc tgggtctgtt tggtacttct tctcacgcta
     6481 aggtggcggt gtgatatgca caatggctaa aaagcaggga gagctggaaa gaaacaagga
     6541 cagagacaga ggccaagtca accagaccaa ttcccagagg aagcaaagaa accattacag
     6601 agactacaag ggggaaggga aggagagatg aattagcttc ccctgtaaac cttagaaccc
     6661 agctgttgcc agggcaacgg ggcaatacct gtctcttcag aggagatgaa gttgccaggg
     6721 taactacatc ctgtctttct caaggaccat cccagaatgt ggcacccact agccgttacc
     6781 atagcaactg cctctttgcc ccacttaatc ccatcccgtc tgttaaaagg gccctatagt
     6841 tggaggtggg ggaggtagga agagcgatga tcacttgtgg actaagtttg ttcgcatccc
     6901 cttctccaac cccctcagta catcaccctg ggggaacagg gtccacttgc tcctgggccc
     6961 acacagtcct gcagtattgt gtatataagg ccagggcaaa gaggagcagg ttttaaagtg
     7021 aaaggcaggc aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag
     7081 gtggttgcca tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt
     7141 gccctgctca gaagccccaa gctcgtcagt caagccggtt ctccgtttgc actcaggagc
     7201 acgggcaggc gagtggcccc tagttctggg ggcagcgggg gatccgccac catggaaaca
     7261 gccgccacaa tgacccacgc ctttatctca gccgtgccta gcgccgaagc cacaattaga
     7321 ggcctgctga gcgccgcagc agtggtgaca ccagcagcag acgctcacgg agaaacctct
     7381 aacgccacaa cagccggagc cgatcacggt tgcttccccc acatcaacca cggaaccgag
     7441 ctgcagcaca agatcgcagt gggactccag tggttcaccg tgatcgtggc tatcgtgcag
     7501 ctcatcttct acggttggca cagcttcaag gccacaaccg gctgggagga ggtctacgtc
     7561 tgcgtgatcg agctcgtcaa gtgcttcatc gagctgttcc acgaggtcga cagcccagcc
     7621 acagtgtacc agaccaacgg aggagccgtg atttggctgc ggtacagcat gtggctcctg
     7681 acttgccccg tgatcctgat ccacctgagc aacctgaccg gactgcacga agagtacagc
     7741 aagcggacca tgaccatcct ggtgaccgac atcggcaaca tcgtgtgggg gatcacagcc
     7801 gcctttacaa agggccccct gaagatcctg ttcttcatga tcggcctgtt ctacggcgtg
     7861 acttgcttct tccagatcgc caaggtgtat atcgagagct accacaccct gcccaaaggc
     7921 gtctgccgga agatttgcaa gatcatggcc tacgtcttct tctgctcttg gctgatgttc
     7981 cccgtgatgt tcatcgccgg acacgaggga ctgggcctga tcacacctta caccagcgga
     8041 atcggccacc tgatcctgga tctgatcagc aagaacactt ggggcttcct gggccaccac
     8101 ctgagagtga agatccacga gcacatcctg atccacggcg acatccggaa gacaaccacc
     8161 atcaacgtgg ccggcgagaa catggagatc gagaccttcg tcgacgagga ggaggaggga
     8221 ggagtggcgg caccggtagt agcagtgagc aagggcgagg agctgttcac cggggtggtg
     8281 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     8341 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatttgcac caccggcaag
     8401 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     8461 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     8521 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     8581 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     8641 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     8701 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     8761 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     8821 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     8881 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     8941 atggacgagc tgtacaagta agaattcgat atcaagctta tcgataatca acctctggat
     9001 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9061 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9121 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     9181 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     9241 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     9301 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     9361 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     9421 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9481 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9541 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     9601 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     9661 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     9721 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9781 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     9841 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     9901 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     9961 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    10021 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
    10081 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10261 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
    10321 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    10381 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    10441 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    10501 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    10561 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    10861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    10921 ggtctattct tttgatttat aagggatt
//
