LOCUS       sequence-173005-        4569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p12 and p9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Myhrvold C, Polka JK, Silver PA
  TITLE     Synthetic Lipid-Containing Scaffolds Enhance Production by 
            Colocalizing Enzymes.
  JOURNAL   ACS Synth Biol. 2016 Aug 3.
  PUBMED    27487319
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Aug 3."
COMMENT     Sequence Label: pCAM17
FEATURES             Location/Qualifiers
     source          1..4569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..321
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     244..263
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             332..343
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1238..1267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1283..1300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1356..1373)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1526..1572
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1614..1631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1785..2373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1865..1884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2544..3335,3336..3404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3167..3186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3405..3509)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3577..3595
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3599..3642)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(join(3701..4569,1..10))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca
       61 tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc
      121 cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca
      181 aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt
      241 gctatgccat agcattttta tccataagat tagcggatct tacctgacgc tttttatcgc
      301 aactctctac tgtttctcca tacccgctag caaagaggag aaaagaatgg ttatcggtct
      361 cctgaagtat ctcacgcctg ccgttaaggt gcagatggct gctcgcgcgt tgggcctgtc
      421 ccccgccgaa gtcgctgcaa ttgacggcac gttgggtcgt gtctctgcga tgccagcggt
      481 cgcggtcgtg ctgggaggga aacctctctc tctggccacg atcgcgtcag ttgtgtctga
      541 tgcaaacccc agtgccactg ttggcgcgct tatgcctgct gtacagggca tggtgagttc
      601 cgacgaaggc gcgagtgcgt tggctaagac cgtggtaggc ttcatggagt ccgaccccaa
      661 cagcgatgtc ctggttcaac tgctccacaa ggtgtcaaac ttgccgattg tcggctttgg
      721 tgacacgcag tatgcagacc cagctgactt cttggccaag ggagttttcc ctctgatcag
      781 gaagccagaa gtagaggttc aagctgcgcc tttcacctgt cgtcagtgtg atcatgttga
      841 tcacatcact gatgtacctc aaacttcgac ctttgttcac aaatgcactt cgtgcggctt
      901 tgtgcagatg gtccaccgta aggatgttcc gtaatgccat ttcctctggt aaagcaagac
      961 ccaacctcga aggctttcac tgaagccagt gaacgctcca ccggcaccca gatcctggac
     1021 gtcgtcaagg cccctatcgg cctgttcggc gacgatgcca aacacgagtt cgtgacccgt
     1081 caggaacaag ccgtctccgt cgtcagctgg gcagttgctg ccggtctgat cggcgagctg
     1141 atcggctacc gtggtgcgcg ttcgggtcgc aaagcgatcc tggccaacat ccctttcctg
     1201 gcctaatcct aactcgagta atgagaagct tgggcccgaa caaaaactca tctcagaaga
     1261 ggatctgaat agcgccgtcg accatcatca tcatcatcat tgagtttaaa cggtctccag
     1321 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     1381 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     1441 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     1501 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1561 ctttcgtttt atctgttgtt tgtcggtgaa ctaatttcta gactgcaggc ttcctcgctc
     1621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     1801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2221 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2461 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2521 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2581 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2641 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2701 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2761 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2821 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2881 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2941 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3001 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3061 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3121 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3181 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3241 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3301 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3361 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3421 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3481 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3541 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     3601 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc actagtctat
     3661 gctactccat cgagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta
     3721 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
     3781 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
     3841 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
     3901 gccagcttaa gacgctaatc cctaactgct ggcggaaaag acgtgacaga cgcgacggcg
     3961 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
     4021 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
     4081 tcgcttccat gcgccgcagc aacaattgct caagcagatt tatcgccagc agctccgaat
     4141 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
     4201 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
     4261 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
     4321 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
     4381 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
     4441 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
     4501 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
     4561 cattttgcg
//
