LOCUS       sequence-17302-e         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland 
            TD, Waugh DS
  TITLE     Efficient site-specific processing of fusion proteins by tobacco 
            vein mottling virus protease in vivo and in vitro.
  JOURNAL   Protein Expr Purif. 2004 Nov . 38(1):108-15.
  PUBMED    15477088
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2004 Nov . 38(1):108-15."
COMMENT     Sequence Label: pRK1035
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctcggaac cgtgcgtttc cgtctcacca ccatcatcat cactctaaag ctttgctgaa
       61 gggcgtgcgc gattttaatc cgatctctgc ttgcgtatgc ctgctggaaa actcctcgga
      121 tggtcatagt gaacgtctgt ttggcattgg ttttggcccg tatatcattg ccaaccagca
      181 tctgtttcgt cgtaacaatg gcgaactgac catcaaaacc atgcatggtg aattcaaagt
      241 caaaaactct acccagctgc agatgaaacc ggttgaaggc cgtgacatta tcgttatcaa
      301 aatggctaaa gacttcccgc cgttcccgca gaaactgaaa ttccgtcagc cgaccatcaa
      361 agatcgtgtg tgcatggtgt ccaccaactt tcagcagaaa agcgtctcga gcctggtgtc
      421 tgaatcctct cacattgtgc ataaagaaga cacttctttc tggcagcact ggatcaccac
      481 taaagatggc cagtgtggca gcccactagt ttccatcatt gatggcaaca ttctgggcat
      541 ccacagcctg actcatacca ccaacggtag caactact
//
