LOCUS       sequence-173023-       13331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 13331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP429
FEATURES             Location/Qualifiers
     source          1..13331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1843..1861
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1843..1860
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             9663..9692
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9699..10244
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             join(11439..11441,11442..11444,11445..12155)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11439..11441,11442..11444,11445..12155)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11484..11505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11745..11764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12092..12113
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      12213..12222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12219..12284
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      12388..12397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12394..13107
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(12539..12557)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12807..12826
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg agctcgtatg gacatattgt cgttagaacg
     1801 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1861 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
     1921 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
     1981 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
     2041 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
     2101 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
     2161 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
     2221 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
     2281 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
     2341 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
     2401 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
     2461 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
     2521 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
     2581 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
     2641 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
     2701 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
     2761 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
     2821 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     2881 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     2941 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     3001 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     3061 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     3121 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     3181 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     3241 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     3301 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     3361 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     3421 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     3481 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     3541 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     3601 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     3661 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     3721 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     3781 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     3841 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     3901 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     3961 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     4021 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     4081 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     4141 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     4201 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     4261 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     4321 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     4381 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     4441 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     4501 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     4561 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     4621 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     4681 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     4741 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     4801 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     4861 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     4921 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     4981 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     5041 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     5101 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     5161 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     5221 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     5281 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     5341 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     5401 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     5461 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     5521 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     5581 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     5641 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     5701 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     5761 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     5821 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     5881 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     5941 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     6001 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     6061 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     6121 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     6181 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     6241 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     6301 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     6361 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     6421 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     6481 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     6541 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     6601 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     6661 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     6721 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     6781 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     6841 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     6901 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     6961 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     7021 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     7081 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     7141 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     7201 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     7261 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     7321 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     7381 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     7441 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     7501 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     7561 ctctacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     7621 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     7681 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     7741 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     7801 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     7861 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     7921 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     7981 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     8041 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     8101 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     8161 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     8221 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     8281 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     8341 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     8401 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     8461 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     8521 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     8581 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     8641 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     8701 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     8761 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     8821 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     8881 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     8941 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     9001 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     9061 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     9121 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     9181 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     9241 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     9301 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     9361 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     9421 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     9481 ctgactaata ccacaacacc accacctcta gagccaccat gtaccagagg atgctccggt
     9541 gcggcgccga tctgggatcg cccgggggcg gcagtggcgg cggcgcaggg gggcgcctgg
     9601 ccctgatctg gatagtcccg ctcaccctcg gcggcctcct aggagtggcc tggggggaat
     9661 tcgaacaaaa actcatctca gaagaggatc tacaattcat ggacaaagac tgcgaaatga
     9721 agcgcaccac cctggatagc cctctgggca agctggaact gtctgggtgc gaacagggcc
     9781 tgcaccgtat catcttcctg ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg
     9841 ccccagccgc cgtgctgggc ggaccagagc cactgatcca ggccaccgcc tggctcaacg
     9901 cctactttca ccagcctgag gccatcgagg agttccctgt gccagccctg caccacccag
     9961 tgttccagca ggagagcttt acccgccagg tgctgtggaa actgctgaaa gtggtgaagt
    10021 tcggagaggt catcagcgag agccacctgg ccgccctggt gggcaatccc gccgccaccg
    10081 ccgccgtgaa caccgccctg gacggaaatc ccgtgcccat tctgatcccc tgccaccggg
    10141 tggtgcaggg cgacagcgac gtggggccct acctgggcgg gctcgccgtg aaagagtggc
    10201 tgctggccca cgagggccac agactgggca agcctgggct gggtgtcgac ggtaccgcat
    10261 ccagtttggg agcgcaccac atccaccatt tccatggcag cagcaagcat cattcagtgc
    10321 ctattgcaat ctacaggtca ccagcatcct tgcgaggcgg acacgctggg acgacgtata
    10381 tctttagcaa aggtggtgga caaattacat ataagtggcc tcctaatgac cgacccagta
    10441 cacgagcaga caggctggcc atcgggttta gcactgttca gaaggaagca gtgttggtgc
    10501 gtgtggacag ttcctcaggc ctgggtgact accttgagct gcacatacac caaggaaaaa
    10561 ttggagttaa atttaatgtt gggacagatg acatcgccat tgaggagtcc aatgcaatca
    10621 ttaatgatgg aaaataccat gtagtgcgtt tcacgaggag tggtggcaat gccacgttac
    10681 aggtggacag ctggccagtc atcgaacgat accctgcagg gcgtcagctc acaatcttca
    10741 atagccaagc aaccataata attggcggga aagagcaggg ccagcccttc cagggccagc
    10801 tctctgggct ttactacaat ggcttgaaag ttctgaatat ggcggcagag aacgatgcca
    10861 acatcgccat agtgggaaat gtgaggctgg tcggtgaagt gccttcctct atgacaactg
    10921 agtcgacagc cactgccatg cagtccgaga tgtccacctc aatcatggag accaccacaa
    10981 ccctggctac cagcacagct cgacgaggaa agccccccac aaaggagcct atcagccaga
    11041 ccacggatga tatccttgtg gcctcggcag agtgtcccag cgacgatgag gacattgacc
    11101 cctgtgagcc gagctcaggt gggttagcca accccacccg agtgggcggc cgcgaaccat
    11161 acccaggctc ggcagaggtg atccgggagt ctagcagtac cactggcatg gtggtgggga
    11221 ttgtcgcagc agcagctctg tgcatcctca tcctcctcta tgccatgtac aagtacagga
    11281 accgggatga agggtcgtac cacgtggatg agagtcgaaa ctacatcagt aactcagcac
    11341 agtccaatgg ggctgtggtc aaggagatgc aacccagcag tgctaaaagt gccaacaaaa
    11401 acaagaagaa caaggataag gagtattatg tcagatctat ggtgagcaag ggcgaggagc
    11461 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
    11521 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
    11581 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
    11641 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
    11701 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
    11761 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
    11821 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
    11881 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
    11941 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
    12001 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
    12061 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    12121 ccgggatcac tctcggcatg gacgagctgt acaagccacc gctcgacgga gccggagctg
    12181 gcgctggagc tggagccgga gctggcggtc tagccaccat ggacgcacaa acacgacgac
    12241 gtgagcgtcg cgctgagaaa caagctcaat ggaaagctgc aaactaggca tgcaggcctt
    12301 aaagcatctc tacggtggtc ctaaatagtc agcatagtac atttcatctg actaatacca
    12361 caacaccacc acctctagta gaggcgcgcc accatggtga gcaagggcga ggaggataac
    12421 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
    12481 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
    12541 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
    12601 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
    12661 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
    12721 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
    12781 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
    12841 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
    12901 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
    12961 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
    13021 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
    13081 ggcatggacg agctgtacaa gtcttagagg ggcccaatga tccgaccagc aaaactcgat
    13141 gtacttccga ggaactgatg tgcataatgc atcaggctgg tacattagat ccccgcttac
    13201 cgcgggcaat atagcaacac taaaaactcg atgtacttcc gaggaagcgc agtgcataat
    13261 gctgcgcagt gttgccacat aaccactata ttaaccattt atctagcgga cgccaaaaac
    13321 tcaatgtatt t
//
