LOCUS       sequence-173028-       14701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14701)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 14701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP427
FEATURES             Location/Qualifiers
     source          1..14701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1908..1926
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1908..1925
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      9578..9587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9725..9751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9758..10303
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      10765..10774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12560..12569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13758..13767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13764..14477
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(13909..13927)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     14177..14196
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggaattcct cgataattaa gcggccgctt aattaatcga ggggaattaa ttcttgaaga
       61 cgaaagggcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcgagctc gtatggacat attgtcgtta
     1861 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     1921 tatagattga cggcgtagta cacactattg aatcaaacag ccgaccaatt gcactaccat
     1981 cacaatggag aagccagtag taaacgtaga cgtagacccc cagagtccgt ttgtcgtgca
     2041 actgcaaaaa agcttcccgc aatttgaggt agtagcacag caggtcactc caaatgacca
     2101 tgctaatgcc agagcatttt cgcatctggc cagtaaacta atcgagctgg aggttcctac
     2161 cacagcgacg atcttggaca taggcagcgc accggctcgt agaatgtttt ccgagcacca
     2221 gtatcattgt gtctgcccca tgcgtagtcc agaagacccg gaccgcatga tgaaatatgc
     2281 cagtaaactg gcggaaaaag cgtgcaagat tacaaacaag aacttgcatg agaagattaa
     2341 ggatctccgg accgtacttg atacgccgga tgctgaaaca ccatcgctct gctttcacaa
     2401 cgatgttacc tgcaacatgc gtgccgaata ttccgtcatg caggacgtgt atatcaacgc
     2461 tcccggaact atctatcatc aggctatgaa aggcgtgcgg accctgtact ggattggctt
     2521 cgacaccacc cagttcatgt tctcggctat ggcaggttcg taccctgcgt acaacaccaa
     2581 ctgggccgac gagaaagtcc ttgaagcgcg taacatcgga ctttgcagca caaagctgag
     2641 tgaaggtagg acaggaaaat tgtcgataat gaggaagaag gagttgaagc ccgggtcgcg
     2701 ggtttatttc tccgtaggat cgacacttta tccagaacac agagccagct tgcagagctg
     2761 gcatcttcca tcggtgttcc acttgaatgg aaagcagtcg tacacttgcc gctgtgatac
     2821 agtggtgagt tgcgaaggct acgtagtgaa gaaaatcacc atcagtcccg ggatcacggg
     2881 agaaaccgtg ggatacgcgg ttacacacaa tagcgagggc ttcttgctat gcaaagttac
     2941 tgacacagta aaaggagaac gggtatcgtt ccctgtgtgc acgtacatcc cggccaccat
     3001 atgcgatcag atgactggta taatggccac ggatatatca cctgacgatg cacaaaaact
     3061 tctggttggg ctcaaccagc gaattgtcat taacggtagg actaacagga acaccaacac
     3121 catgcaaaat taccttctgc cgatcatagc acaagggttc agcaaatggg ctaaggagcg
     3181 caaggatgat cttgataacg agaaaatgct gggtactaga gaacgcaagc ttacgtatgg
     3241 ctgcttgtgg gcgtttcgca ctaagaaagt acattcgttt tatcgcccac ctggaacgca
     3301 gacctgcgta aaagtcccag cctcttttag cgcttttccc atgtcgtccg tatggacgac
     3361 ctctttgccc atgtcgctga ggcagaaatt gaaactggca ttgcaaccaa agaaggagga
     3421 aaaactgctg caggtctcgg aggaattagt catggaggcc aaggctgctt ttgaggatgc
     3481 tcaggaggaa gccagagcgg agaagctccg agaagcactt ccaccattag tggcagacaa
     3541 aggcatcgag gcagccgcag aagttgtctg cgaagtggag gggctccagg cggacatcgg
     3601 agcagcatta gttgaaaccc cgcgcggtca cgtaaggata atacctcaag caaatgaccg
     3661 tatgatcgga cagtatatcg ttgtctcgcc aaactctgtg ctgaagaatg ccaaactcgc
     3721 accagcgcac ccgctagcag atcaggttaa gatcataaca cactccggaa gatcaggaag
     3781 gtacgcggtc gaaccatacg acgctaaagt actgatgcca gcaggaggtg ccgtaccatg
     3841 gccagaattc ctagcactga gtgagagcgc cacgttagtg tacaacgaaa gagagtttgt
     3901 gaaccgcaaa ctataccaca ttgccatgca tggccccgcc aagaatacag aagaggagca
     3961 gtacaaggtt acaaaggcag agcttgcaga aacagagtac gtgtttgacg tggacaagaa
     4021 gcgttgcgtt aagaaggaag aagcctcagg tctggtcctc tcgggagaac tgaccaaccc
     4081 tccctatcat gagctagctc tggagggact gaagacccga cctgcggtcc cgtacaaggt
     4141 cgaaacaata ggagtgatag gcacaccggg gtcgggcaag tcagctatta tcaagtcaac
     4201 tgtcacggca cgagatcttg ttaccagcgg aaagaaagaa aattgtcgcg aaattgaggc
     4261 cgacgtgcta agactgaggg gtatgcagat tacgtcgaag acagtagatt cggttatgct
     4321 caacggatgc cacaaagccg tagaagtgct gtacgttgac gaagcgttcg cgtgccacgc
     4381 aggagcacta cttgccttga ttgctatcgt caggccccgc aagaaggtag tactatgcgg
     4441 agaccccatg caatgcggat tcttcaacat gatgcaacta aaggtacatt tcaatcaccc
     4501 tgaaaaagac atatgcacca agacattcta caagtatatc tcccggcgtt gcacacagcc
     4561 agttacagct attgtatcga cactgcatta cgatggaaag atgaaaacca cgaacccgtg
     4621 caagaagaac attgaaatcg atattacagg ggccacaaag ccgaagccag gggatatcat
     4681 cctgacatgt ttccgcgggt gggttaagca attgcaaatc gactatcccg gacatgaagt
     4741 aatgacagcc gcggcctcac aagggctaac cagaaaagga gtgtatgccg tccggcaaaa
     4801 agtcaatgaa aacccactgt acgcgatcac atcagagcat gtgaacgtgt tgctcacccg
     4861 cactgaggac aggctagtgt ggaaaacctt gcagggcgac ccatggatta agcagctcac
     4921 taacatacct aaaggaaact ttcaggctac tatagaggac tgggaagctg aacacaaggg
     4981 aataattgct gcaataaaca gccccactcc ccgtgccaat ccgttcagct gcaagaccaa
     5041 cgtttgctgg gcgaaagcat tggaaccgat actagccacg gccggtatcg tacttaccgg
     5101 ttgccagtgg agcgaactgt tcccacagtt tgcggatgac aaaccacatt cggccattta
     5161 cgccttagac gtaatttgca ttaagttttt cggcatggac ttgacaagcg gactgttttc
     5221 taaacagagc atcccactaa cgtaccatcc cgccgattca gcgaggccgg tagctcattg
     5281 ggacaacagc ccaggaaccc gcaagtatgg gtacgatcac gccattgccg ccgaactctc
     5341 ccgtagattt ccggtgttcc agctagctgg gaagggcaca caacttgatt tgcagacggg
     5401 gagaaccaga gttatctctg cacagcataa cctggtcccg gtgaaccgca atcttcctca
     5461 cgccttagtc cccgagtaca aggagaagca acccggcccg gtcgaaaaat tcttgaacca
     5521 gttcaaacac cactcagtac ttgtggtatc agaggaaaaa attgaagctc cccgtaagag
     5581 aatcgaatgg atcgccccga ttggcatagc cggtgcagat aagaactaca acctggcttt
     5641 cgggtttccg ccgcaggcac ggtacgacct ggtgttcatc aacattggaa ctaaatacag
     5701 aaaccaccac tttcagcagt gcgaagacca tgcggcgacc ttaaaaaccc tttcgcgttc
     5761 ggccctgaat tgccttaacc caggaggcac cctcgtggtg aagtcctatg gctacgccga
     5821 ccgcaacagt gaggacgtag tcaccgctct tgccagaaag tttgtcaggg tgtctgcagc
     5881 gagaccagat tgtgtctcaa gcaatacaga aatgtacctg attttccgac aactagacaa
     5941 cagccgtaca cggcaattca ccccgcacca tctgaattgc gtgatttcgt ccgtgtatga
     6001 gggtacaaga gatggagttg gagccgcgcc gtcataccgc accaaaaggg agaatattgc
     6061 tgactgtcaa gaggaagcag ttgtcaacgc agccaatccg ctgggtagac caggcgaagg
     6121 agtctgccgt gccatctata aacgttggcc gaccagtttt accgattcag ccacggagac
     6181 aggcaccgca agaatgactg tgtgcctagg aaagaaagtg atccacgcgg tcggccctga
     6241 tttccggaag cacccagaag cagaagcctt gaaattgcta caaaacgcct accatgcagt
     6301 ggcagactta gtaaatgaac ataacatcaa gtctgtcgcc attccactgc tatctacagg
     6361 catttacgca gccggaaaag accgccttga agtatcactt aactgcttga caaccgcgct
     6421 agacagaact gacgcggacg taaccatcta ttgcctggat aagaagtgga aggaaagaat
     6481 cgacgcggca ctccaactta aggagtctgt aacagagctg aaggatgaag atatggagat
     6541 cgacgatgag ttagtatgga ttcatccaga cagttgcttg aagggaagaa agggattcag
     6601 tactacaaaa ggaaaattgt attcgtactt cgaaggcacc aaattccatc aagcagcaaa
     6661 agacatggcg gagataaagg tcctgttccc taatgaccag gaaagtaatg aacaactgtg
     6721 tgcctacata ttgggtgaga ccatggaagc aatccgcgaa aagtgcccgg tcgaccataa
     6781 cccgtcgtct agcccgccca aaacgttgcc gtgcctttgc atgtatgcca tgacgccaga
     6841 aagggtccac agacttagaa gcaataacgt caaagaagtt acagtatgct cctccacccc
     6901 ccttcctaag cacaaaatta agaatgttca gaaggttcag tgcacgaaag tagtcctgtt
     6961 taatccgcac actcccgcat tcgttcccgc ccgtaagtac atagaagtgc cagaacagcc
     7021 taccgctcct cctgcacagg ccgaggaggc ccccgaagtt gtagcgacac cgtcaccatc
     7081 tacagctgat aacacctcgc ttgatgtcac agacatctca ctggatatgg atgacagtag
     7141 cgaaggctca cttttttcga gctttagcgg atcggacaac tctattacta gtatggacag
     7201 ttggtcgtca ggacctagtt cactagagat agtagaccga aggcaggtgg tggtggctga
     7261 cgttcatgcc gtccaagagc ctgcccctat tccaccgcca aggctaaaga agatggcccg
     7321 cctggcagcg gcaagaaaag agcccactcc accggcaagc aatagctctg agtccctcca
     7381 cctctctttt ggtggggtat ccatgtccct cggatcaatt ttcgacggag agacggcccg
     7441 ccaggcagcg gtacaacccc tggcaacagg ccccacggat gtgcctatgt ctttcggatc
     7501 gttttccgac ggagagattg atgagctgag ccgcagagta actgagtccg aacccgtcct
     7561 gtttggatca tttgaaccgg gcgaagtgaa ctcaattata tcgtcccgat cagccgtatc
     7621 ttttcctcta cgcaagcaga gacgtagacg caggagcagg aggactgaat actgactaac
     7681 cggggtaggt gggtacatat tttcgacgga cacaggccct gggcacttgc aaaagaagtc
     7741 cgttctgcag aaccagctta cagaaccgac cttggagcgc aatgtcctgg aaagaattca
     7801 tgccccggtg ctcgacacgt cgaaagagga acaactcaaa ctcaggtacc agatgatgcc
     7861 caccgaagcc aacaaaagta ggtaccagtc tcgtaaagta gaaaatcaga aagccataac
     7921 cactgagcga ctactgtcag gactacgact gtataactct gccacagatc agccagaatg
     7981 ctataagatc acctatccga aaccattgta ctccagtagc gtaccggcga actactccga
     8041 tccacagttc gctgtagctg tctgtaacaa ctatctgcat gagaactatc cgacagtagc
     8101 atcttatcag attactgacg agtacgatgc ttacttggat atggtagacg ggacagtcgc
     8161 ctgcctggat actgcaacct tctgccccgc taagcttaga agttacccga aaaaacatga
     8221 gtatagagcc ccgaatatcc gcagtgcggt tccatcagcg atgcagaaca cgctacaaaa
     8281 tgtgctcatt gccgcaacta aaagaaattg caacgtcacg cagatgcgtg aactgccaac
     8341 actggactca gcgacattca atgtcgaatg ctttcgaaaa tatgcatgta atgacgagta
     8401 ttgggaggag ttcgctcgga agccaattag gattaccact gagtttgtca ccgcatatgt
     8461 agctagactg aaaggcccta aggccgccgc actatttgca aagacgtata atttggtccc
     8521 attgcaagaa gtgcctatgg atagattcgt catggacatg aaaagagacg tgaaagttac
     8581 accaggcacg aaacacacag aagaaagacc gaaagtacaa gtgatacaag ccgcagaacc
     8641 cctggcgact gcttacttat gcgggattca ccgggaatta gtgcgtaggc ttacggccgt
     8701 cttgcttcca aacattcaca cgctttttga catgtcggcg gaggattttg atgcaatcat
     8761 agcagaacac ttcaagcaag gcgacccggt actggagacg gatatcgcat cattcgacaa
     8821 aagccaagac gacgctatgg cgttaaccgg tctgatgatc ttggaggacc tgggtgtgga
     8881 tcaaccacta ctcgacttga tcgagtgcgc ctttggagaa atatcatcca cccatctacc
     8941 tacgggtact cgttttaaat tcggggcgat gatgaaatcc ggaatgttcc tcacactttt
     9001 tgtcaacaca gttttgaatg tcgttatcgc cagcagagta ctagaagagc ggcttaaaac
     9061 gtccagatgt gcagcgttca ttggcgacga caacatcata catggagtag tatctgacaa
     9121 agaaatggct gagaggtgcg ccacctggct caacatggag gttaagatca tcgacgcagt
     9181 catcggtgag agaccacctt acttctgcgg cggatttatc ttgcaagatt cggttacttc
     9241 cacagcgtgc cgcgtggcgg atcccctgaa aaggctgttt aagttgggta aaccgctccc
     9301 agccgacgac gagcaagacg aagacagaag acgcgctctg ctagatgaaa caaaggcgtg
     9361 gtttagagta ggtataacag gcactttagc agtggccgtg acgacccggt atgaggtaga
     9421 caatattaca cctgtcctac tggcattgag aacttttgcc cagagcaaaa gagcattcca
     9481 agccatcaga ggggaaataa agcatctcta cggtggtcct aaatagtcag catagtacat
     9541 ttcatctgac taataccaca acaccaccac ctctagagcc accatggcac ttcccagatg
     9601 catgtggcca aattatgttt ggagagctat gatggcatgt gtggtccaca ggggatccgg
     9661 tgccccattg actctctgct tgttgggatg tttgctacag acttttcacg tactctctca
     9721 aaagtatcca tacgacgttc cggactacgc agaattcatg gacaaagact gcgaaatgaa
     9781 gcgcaccacc ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct
     9841 gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc
     9901 cccagccgcc gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc
     9961 ctactttcac cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt
    10021 gttccagcag gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt
    10081 cggagaggtc atcagctaca gccacctggc cgccctggcc ggcaatcccg ccgccaccgc
    10141 cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt
    10201 ggtgcagggc gacctggacg tggggggcta cgagggcggg ctcgccgtga aagagtggct
    10261 gctggcccac gagggccaca gactgggcaa gcctgggctg ggtgtcgact tggatgatgt
    10321 agacccattg gttactacta actttggcaa gattagggga attaagaaag aactcaataa
    10381 tgaaattttg ggtcctgtca ttcagtttct tggggttcca tatgccgctc caccaacagg
    10441 agaacatcgt ttccagcctc cagaaccacc atctccctgg tctgacatcc ggaacgccac
    10501 tcagtttgct cctgtatgtc cccagaatat cattgatggc agattgcctg aagttatgct
    10561 tcctgtgtgg ttcactaata acttggatgt ggtttcatca tacgtccaag accagagtga
    10621 agactgtcta tacttaaaca tctatgtccc aactgaagat ggtcccctta caaagaaaca
    10681 cacagatgat ttaggtgata atgacggtgc tgaagatgaa gatattcggg acagtggggg
    10741 tcccaaacca gtgatggtgt acatccatgg cggctcttac atggaaggta ctggaaatct
    10801 gtatgatggg agtgtcttgg caagctatgg caatgtgatc gtcatcacag tcaactatcg
    10861 gcttggggta cttggcttct tgagcacagg ggatcaggct gccaaaggaa actacgggct
    10921 ccttgacctc atccaggccc taagatggac cagcgagaac attgggttct ttggtggtga
    10981 ccccttgcga atcactgtgt ttggatcagg cgctgggggt tcatgtgtca acctgctgac
    11041 tttatcccat tattctgaag gtaaccgttg gagcaattca accaaaggac tttttcaacg
    11101 agcaatagct cagagtggaa cagccctttc cagctgggct gttagtttcc agcctgcaaa
    11161 atacgctaga attctggcca caaaagttgg ctgcaatgtt tcagatacag tagagttagt
    11221 agaatgcctg cagaagaagc cttacaaaga acttgttgat caagatgttc aaccagcccg
    11281 ataccacata gcctttggac ctgtgatcga tggtgatgta ataccagatg accctcagat
    11341 actgatggaa caaggagagt tcctcaacta tgatataatg ttgggagtta accaagggga
    11401 agggttgaag tttgtcgaaa atatagtaga tagtgatgat ggtgtatcag ccagtgattt
    11461 cgactttgct gtttctaatt ttgttgataa tttatatgga tatcctgaag gcaaagatgt
    11521 tttgagagaa accattaaat tcatgtatac tgactgggct gatcgccata atcctgaaac
    11581 tagaaggaag acattgttgg ctttgtttac ggaccatcaa tgggtagcac ctgctgtggc
    11641 cacagcagac cttcactcga actttggctc acctacatac ttctatgcct tttatcatca
    11701 ttgccaaaca gatcaagttc cagcttgggc tgatgcagct catggggatg aggttcccta
    11761 tgtgttggga atccccatga ttggccctac agagttattt ccttgcaatt tctccaagaa
    11821 tgatgtgatg ttgagtgcag tagtaatgac atactggacg aattttgcta aaactggtga
    11881 cccaaatcaa ccagttcctc aagacacaaa attcatccat accaaaccca accgctttga
    11941 agaagtagca tggaccagat attcccagaa agaccagctt tatctccata ttggattaaa
    12001 accgagagtt aaagagcatt acagagccaa taaggtaaat ctctggctgg agctggtacc
    12061 tcatctgcat aatctcaatg acatttctca gtatacctcg acaacaacta aagtgccatc
    12121 cacggacatc actctcagac ctacaaggaa gaattccaca ccagtcacat cagcctttcc
    12181 cactgccaaa caggatgatc ccaagcaaca accaagcccc ttctcggtgg atcagaggga
    12241 ctactccaca gagctaagtg tcactatcgc agtgggggcc tctctgctgt ttctcaacat
    12301 cttggctttt gcagccctgt actacaagaa ggataagagg agacatgatg tccaccggag
    12361 gtgcagccct cagcgcacga ccaccaacga cctaacccat gctccagaag aggaaattat
    12421 gtctctccaa atgaagcaca ctgacttgga tcacgagtgt gagtccatcc atccacatga
    12481 ggtggttctt cggaccagcg ctccaccgct cgacggagcc ggagctggcg ctggagctgg
    12541 agccggagct ggcgggctag ccaccatggc cagcaacttc acccagtttg tgctggtgga
    12601 caatggcggg acaggcgatg tgactgtggc tccctccaac ttcgccaatg gggtggctga
    12661 gtggatcagc tccaacagtc ggtcacaggc ctacaaggtg acctgcagcg tgcggcagtc
    12721 tagtgctcag aagagaaagt acacaattaa ggtggaggtg cccaaagtgg ccacccagac
    12781 agtgggagga gtggaactgc ctgtggctgc tcggagatcc tacctgaaca tggagctgac
    12841 tatccctatt ttcgccacca attctgactg tgaactgatc gtgaaggcta tgcagggact
    12901 gctgaaagat ggcaacccca tcccttctgc cattgccgct aatagtggaa tctatggcgc
    12961 ccctgggatt cacccaggga tgatggcttc taacttcacc caatttgtgc tggtcgataa
    13021 tgggggaacc ggagatgtga cagtggcccc aagtaacttt gccaatggcg tggctgaatg
    13081 gatctcaagc aatagccggt cccaggccta caaagtgact tgctccgtga ggcagtcctc
    13141 tgctcagaag cgcaaatata caattaaggt cgaagtgcca aaagtggcca ctcagaccgt
    13201 cggcggagtg gaactgcccg tggctgctag gcgcagttac ctgaatatgg aactgacaat
    13261 ccctattttt gccactaatt cagattgcga gctgattgtg aaggctatgc aggggctgct
    13321 gaaagatgga aacccaatcc cctcagccat tgccgctaat agcggcatct acctcgagcc
    13381 accgctcgac ggagccggag ctggcgctgg agctggagcc ggagctggcg gtctagccac
    13441 cccaccgagc gctgcctgtc ccccagatta tactctagct atgaggaggt cacctgatga
    13501 tattccacta atgacaccta acaccatcac aatgattccc aacactatac cagggattca
    13561 gcccttacat acattcaaca catttactgg aggacagaat aatacactgc cccatcccca
    13621 cccacacccc cattcacatt caacaaccag ggtataggca tgcaggcctt aaagcatctc
    13681 tacggtggtc ctaaatagtc agcatagtac atttcatctg actaatacca caacaccacc
    13741 acctctagta gaggcgcgcc accatggtga gcaagggcga ggaggataac atggccatca
    13801 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
    13861 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
    13921 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
    13981 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
    14041 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
    14101 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
    14161 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
    14221 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
    14281 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
    14341 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
    14401 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
    14461 agctgtacaa gtcttagagg ggcccaatga tccgaccagc aaaactcgat gtacttccga
    14521 ggaactgatg tgcataatgc atcaggctgg tacattagat ccccgcttac cgcgggcaat
    14581 atagcaacac taaaaactcg atgtacttcc gaggaagcgc agtgcataat gctgcgcagt
    14641 gttgccacat aaccactata ttaaccattt atctagcgga cgccaaaaac tcaatgtatt
    14701 t
//
